Dataset DOI: 10.5061/dryad.5qfttdzmh

Description of the data and file structure

Obesity and sarcopenia affect the metabolite profiles of pet dogs.

Files and variables

File: Code_Eloetal.R

Description: Includes all codes utilised to analyse the data.

File: Data_Eloetal.txt

Description: Includes the physiological information and metabolite concentrations of dogs. This file is needed to run the analyses.

Variables Form: Variable name / Variable label / Variable values

• Dog / numeric id of the dog / factor, values 1-187
• Age / age of the dog when blood sample was taken / continuous, values 2-16.37
• Sex / sex of the dog / categorical; bitch or male
• Castrated / castration status of the male dog / categorical; yes: neutered and no: intact
• Sterilised / sterilisation status of the dog / categorical; yes: sterilised and no: intact
• Fasting / time fasted before blood sampling / categorical; less than 4 hours, 4-7 hours, 8-11 hours, or 12 hours or more
• BCS / WSAVA body condition score of the dog / categorical; 3, 4, 5, 6, 7, 8 and 9
• MCS / WSAVA muscle condition score of the dog / categorical; 1: normal muscle mass, 2: mild muscle loss, 3: moderate muscle loss, or 4: severe muscle loss
• Diet / type of diet consumed by the dog / categorical; commercial diet, partly raw diet partly commercial diet, raw diet, home-cooked diet, or other
• Feeding frequency / how many times a day the dog is fed / categorical; once a day, twice a day, three times a day or more, ad libitum
• Feeding amount / how is the daily feeding amount determined / categorical; By checking dog's weight and body condition, Instructed by veterinarian, According to dog's perceived hunger, ad libitum, According to the package instructions of commercial diet
• Treats / how often does the dog get treats / categorical; every day, few times a week, few times a month, never
• Oil / how often does the dog get oil supplement / categorical; every day, few times a week, few times a month, few times a year, never
• Exercise amount / how many hours per day does the dog get exercise / categorical; under 30 min, 30min - 1h, 1 - 2 hours, 2 - 3 hours, 3 hours or more
• Exercise type / does the dog exercise on leash or off-leash / categorical; on leash, off-leash, on leash and off-leash
• Health status / does the dog have any form of disease / categorical; healthy or disease
• Diagnosis / what is the diagnosis of dogs reported as "disease" in the "Health status" variable / free text
• Total.C.y / Total cholesterol (mmol/l) / continuous
• VLDL.C.y / VLDL cholesterol (mmol/l) / continuous
• LDL.C.y / LDL cholesterol (mmol/l) / continuous
• HDL.C.y / HDL cholesterol (mmol/l) / continuousTotal.TG.y:
• VLDL.TG.y / VLDL triglycerides (mmol/l) / continuous
• LDL.TG.y / LDL triglycerides (mmol/l) / continuous
• HDL.TG.y / HDL triglycerides (mmol/l) / continuous
• Esterified.C.y / Esterified cholesterol (mmol/l) / continuous
• Free.C.y / Free cholesterol (mmol/l) / continuous
• VLDL.L.y / VLDL lipids (mmol/l) / continuous
• LDL.L.y / LDL lipids (mmol/l) / continuous
• HDL.L.y / HDL lipids (mmol/l) / continuous
• VLDL.P.y / VLDL particles (mmol/l) / continuous
• LDL.P.y / LDL particles (mmol/l) / continuous
• HDL.P.y / HDL particles (mmol/l) / continuous
• VLDL.size.y / VLDL diameter (nm) / continuous
• LDL.size.y / LDL diameter (nm) / continuous
• HDL.size.y / HDL diameter (nm) / continuous
• Total.FA.y / Total fatty acids (mmol/l) / continuous
• Omega.3.y / Omega-3 fatty acids (mmol/l) / continuous
• Omega.6.y / Omega-6 fatty acids (mmol/l) / continuous
• PUFA.y / Polyunsaturated fatty acids (mmol/l) / continuous
• SFA.y / Saturated fatty acids (mmol/l) / continuous
• PalA.y / Palmitic acid (mmol/l) / continuous
• SteA.y / Stearic acid (mmol/l) / continuous
• OleA.y / Oleic acid (mmol/l) / continuous
• LA.y / Linoleic acid (mmol/l) / continuous
• AA.y / Arachidonic acid (mmol/l) / continuous
• DPA.y / Docosapentaenoic (mmol/l) / continuous
• DHA.y / Docosapentaenoic (mmol/l) / continuous
• Omega.3.pros.y / Omega-3 fatty acid ratio (%) / continuous
• Omega.6.pros.y / Omega-6 fatty acid ratio (%) / continuous
• PUFA.pros.y / Polyunsaturated fatty acid ratio (%) / continuous
• SFA.pros.y / Saturated fatty acid ratio (%) / continuous
• PalA.pros.y / Palmitic acid ratio (%) / continuous
• SteA.pros.y / Stearic acid ratio (%) / continuous
• OleA.pros.y / Oleic acid ratio (%) / continuous
• LA.pros.y / Linoleic acid ratio (%) / continuous
• AA.pros.y / Arachidonic acid ratio (%) / continuous
• DPA.pros.y / Docosapentaenoic acid ratio (%) / continuous
• DHA.pros.y / Docosahexaenoic acid ratio (%) / continuous
• Omega.6.Omega.3.y / Ratio of omega-6 fatty acids to omega-3 fatty acids / continuous
• Ala.y / Alanine (mmol/l) / continuous
• Gln.y / Glycine (mmol/l) / continuous
• Gly.y / Glutamine (mmol/l) / continuous
• His.y / Histidine (mmol/l) / continuous
• Total.BCAA.y / Total branched-chain amino acids (mmol/l) / continuous
• Ile.y / Isoleucine (mmol/l) / continuous
• Leu.y / Leucine (mmol/l) / continuous
• Val.y / Valine (mmol/l) / continuous
• Phe.y / Phenylalanine (mmol/l) / continuous
• Tyr.y / Tyrosine (mmol/l) / continuous
• Gly.BCAA.y / Ratio of glycine to total branched-chain amino acids / continuous
• BCAA.Tyr.y / Ratio of branched-chain amino acids to tyrosine / continuous
• Phe.Tyr.y / Ratio of phenylalanine to tyrosine / continuous
• Gly.Val.y / Ratio of glycine to valine / continuous
• Ala.BCAA.y / Ratio of alanine to total branched-chain amino acids / continuous
• Ala.Val.y / Ratio of alanine to valine/ continuous
• Glucose.y / Glucose (mmol/l) / continuous
• Lactate.y / Lactate (mmol/l) / continuous
• Pyruvate.y / Pyruvate (mmol/l) / continuous
• Citrate.y / Citrate (mmol/l) / continuous
• Acetate.y / Acetate (mmol/l) / continuous
• Creatinine.y / Creatinine (mmol/l) / continuous
• Albumin.y / Albumin (g/l) / continuous
• GlycA.y / Glycoprotein acetyls (mmol/l) / continuous
• XL.VLDL.P.y / Very large VLDL particles (mmol/l) / continuous
• XL.VLDL.L.y / Total lipids in very large VLDL particles (mmol/l) / continuous
• XL.VLDL.C.y / Cholesterol in very large VLDL particles (mmol/l) / continuous
• XL.VLDL.TG.y / Triglycerides in very large VLDL particles (mmol/l) / continuous
• L.VLDL.P.y / Large VLDL particles (mmol/l) / continuous
• L.VLDL.L.y / Total lipids in large VLDL particles (mmol/l) / continuous
• L.VLDL.PL.y / Phospholipids in large VLDL particles (mmol/l) / continuous
• L.VLDL.C.y / Cholesterol in large VLDL particles (mmol/l) / continuous
• L.VLDL.CE.y / Esterified cholesterol in large VLDL particles (mmol/l) / continuous
• L.VLDL.FC.y / Free cholesterol in large VLDL particles (mmol/l) / continuous
• L.VLDL.TG.y / Triglycerides in large VLDL particles (mmol/l) / continuous
• S.VLDL.P.y / Small VLDL particles (mmol/l) / continuous
• S.VLDL.L.y / Total lipids in small VLDL particles (mmol/l) / continuous
• S.VLDL.PL.y / Phospholipids in small VLDL particles (mmol/l) / continuous
• S.VLDL.C.y / Cholesterol in small VLDL particles (mmol/l) / continuous
• S.VLDL.CE.y / Esterified cholesterol in small VLDL particles (mmol/l) / continuous
• S.VLDL.FC.y / Free cholesterol in small VLDL particles (mmol/l) / continuous
• S.VLDL.TG.y / Triglycerides in small VLDL particles (mmol/l) / continuous
• L.LDL.P.y / Large LDL particles (mmol/l) / continuous
• L.LDL.L.y / Total lipids in large LDL particles (mmol/l) / continuous
• L.LDL.PL.y / Phospholipids in large LDL particles (mmol/l) / continuous
• L.LDL.C.y / Cholesterol in large LDL particles (mmol/l) / continuous
• L.LDL.CE.y / Esterified cholesterol in large LDL particles (mmol/l) / continuous
• L.LDL.FC.y / Free cholesterol in large LDL particles (mmol/l) / continuous
• L.LDL.TG.y / Triglycerides in large LDL particles (mmol/l) / continuous
• S.LDL.P.y / Small LDL particles (mmol/l) / continuous
• S.LDL.L.y / Total lipids in small LDL particles (mmol/l) / continuous
• S.LDL.PL.y / Phospholipids in small LDL particles (mmol/l) / continuous
• S.LDL.C.y / Cholesterol in small LDL particles (mmol/l) / continuous
• S.LDL.CE.y / Esterified cholesterol in large LDL particles (mmol/l) / continuous
• S.LDL.FC.y / Free cholesterol in large LDL particles (mmol/l) / continuous
• S.LDL.TG.y / Triglycerides in small LDL particles (mmol/l) / continuous
• XL.HDL.P.y / Very large HDL particles (mmol/l) / continuous
• XL.HDL.L.y / Total lipids in very large HDL particles (mmol/l) / continuous
• XL.HDL.PL.y / Phospholipids in very large HDL particles (mmol/l) / continuous
• XL.HDL.C.y / Cholesterol in very large HDL particles (mmol/l) / continuous
• XL.HDL.CE.y / Esterified cholesterol in very large HDL particles (mmol/l) / continuous
• XL.HDL.FC.y / Free cholesterol in very large HDL particles (mmol/l) / continuous
• XL.HDL.TG.y / Triglycerides in very large HDL particles (mmol/l) / continuous
• L.HDL.P.y / Large HDL particles (mmol/l) / continuous
• L.HDL.L.y / Total lipids in large HDL particles (mmol/l) / continuous
• L.HDL.PL.y / Phospholipids in large HDL particles (mmol/l) / continuous
• L.HDL.C.y / Cholesterol in large HDL particles (mmol/l) / continuous
• L.HDL.CE.y / Esterified cholesterol in large HDL particles (mmol/l) / continuous
• L.HDL.FC.y / Free cholesterol in large HDL particles (mmol/l) / continuous
• L.HDL.TG.y / Triglycerides in large HDL particles (mmol/l) / continuous
• S.HDL.P.y / Small HDL particles (mmol/l) / continuous
• S.HDL.L.y / Total lipids in small HDL particles (mmol/l) / continuous
• S.HDL.PL.y / Phospholipids in small HDL particles (mmol/l) / continuous
• S.HDL.C.y / Cholesterol in small HDL particles (mmol/l) / continuous
• S.HDL.CE.y / Esterified cholesterol in small HDL particles (mmol/l) / continuous
• S.HDL.FC.y / Free cholesterol in small HDL particles (mmol/l) / continuous
• S.HDL.TG.y / Triglycerides in small HDL particles (mmol/l) / continuous

File: BCS_MAD_forheatmap.txt

Description: Includes data that was utilised to create a circular heatmap illustrating metabolite concentrations per BCS group. The data frame holds the average metabolite concentrations in each BCS group as MAD from median.

Variables

• BCS_8: Body condition score 8 to 9
• BCS7: Body condition score 7
• BCS6: Body condition score 6
• BCS5: Body condition score 5
• BCS4: Body condition score 4
• class: Metabolite category

File: MCS_mad_forheatmap.txt

Description: Includes data that was utilised to create a circular heatmap illustrating metabolite concentrations per MCS group. The data frame holds the average metabolite concentrations in each MCS group as MAD from median.

Variables

• MCS_3: Moderate to severe muscle loss
• MCS2: Mild muscle loss
• MCS1: Normal muscle mass
• class: Metabolite category

File: Heatmap_pvalues.txt

Description: Includes he input data for the significance heatmap includes a table of the overall significances of the different explanatory variables on the different metabolite measurands.

Variables

• Group: Metabolite category
• Metabolite: p-value for metabolite
• MCS: p-value for muscle condition score
• Health: p-value for health status
• Age: p-value for age
• Agesquared: p-value for quadratic age if included in the model

Code/software

The analyses were run in R (R Core Team. R: A language and environment for statistical computing. [Internet]. Vienna, Austria: R Foundation for Statistical Computing; 2023. Available from: https://www.r-project.org/). All the needed packages for running the analyses are provided in Code_Eloetal.R.