Genetic diversity and population genetic structure from different host populations of Spodoptera litura based on microsatellite markers
Data files
Oct 30, 2025 version files 46.33 KB
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DATA1.xlsx
22.02 KB
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DATA2.xlsx
20.78 KB
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README.md
3.52 KB
Abstract
Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is an agricultural pest with a wide distribution range and many host plants, which can cause serious economic losses. In this study, 157 samples were collected from 8 hosts (tobacco, soybean, taro, peanut, chili, sweet potatoes, corn, and sorrel) in Fuli Town, Fuchuan County, Hezhou City, Guangxi Zhuang Autonomous Region. Seven microsatellite loci were used to analyze the genetic diversity, genetic structure, and genetic differentiation of these populations. The results showed that the number of alleles (Na) of 8 populations ranged from 2.43 to 6.14, the average of polymorphism information content (PIC) was 0.4574, the mean of observed heterozygosity (HO) and expected heterozygosity (HE) were 0.4926 and 0.5693, respectively. The genetic diversity of different host populations was high. Based on the Bayesian clustering and NJ phylogenetic tree of genetic structure, it was shown that there were certain genetic structures among different host populations, which could be roughly divided into four genetic groups. The fixed coefficient (FST) between populations ranged from 0.010 to 0.208, with low to highly differentiated populations as a whole. This study is helpful to understand the occurrence regularity of Spodoptera litura from different host plants, and provides a theoretical basis for the reasonable adjustment of crop layout in the field and the establishment of a corresponding comprehensive control system.
Dataset DOI: 10.5061/dryad.6djh9w1f2
Description of the data and file structure
These data are microsatellite results from 157 individuals of eight host populations of Spodoptera litura. The samples of different hosts were collected from Fuli Town, Fuchuan County, Hezhou City, Guangxi Zhuang Autonomous Region (111°23´E, 24°53´N), and a total of 157 larvae of 8-24 were taken from each host (Table 1), which were tobacco (YC), soybean (DD), taro (XY), peanut (HS), chili (LJ), sweet potato (XY), corn (YM), and sorrel (SM) populations. Genomic DNA was extracted from the head of each larva using Ezup Column Animal Genomic DNA Purification Kit (Shanghai, China) according to the manufacturer’s instructions. 157 samples were amplified by PCR using seven pairs of microsatellite primers (CWM−1, CWM−4, CWM−5, CWM−6, CWM−7, CWM−8, and CWM−9) (Wu et al., 2019). PCR reaction 25 μL: 2×Taq PCR Master Mix 13 μL, DNA template 1 μL, forward and reverse primers 1 μL each, ddH2O 9 μL. Amplification program: pre-denaturation at 95°C for 4 min, denaturation at 95°C for 30 s, followed by 30 cycles of 30 s at 94°C, annealing at 46-58°C for 30 s, and finally extension at 72°C for 7 min. The PCR products were qualified by electrophoresis and sent to Sangon Biotech (Shanghai, China) for sequencing.
Files and variables
File: DATA1.xlsx and DATA2.xlsx (The original data of the two files is the same, but the software used and the results are different, so the required data formats are not the same）
Description: POP refers to population: There are a total of eight host populations, namely: tobacco, soybean, taro, peanut, chili, sweet potatoes, corn, and sorrel.
Samples refer to all individuals from different host populations: 24 from the tobacco population, 24 from the taro population, 24 from the peanut population, 23 from the sweet potato population, 20 from the sorrel population, 20 from the corn population, 14 from the soybean population, and 8 from the chili population.
There are a total of 7 microsatellite primers, namely: CWM−1, CWM−4, CWM−5, CWM−6, CWM−7, CWM−8, and CWM−9.
Variables
- A result with a value of 0 indicates that no peak value has been read at this position, and the result is 0.
- n, sample size; NA, number of alleles; HO, observed heterogeneity; HE, expected heterogeneity; PIC, polymorphism information content; I, Shannon’s index; FIS, inbreeding coefficient.
Code/software
The number of alleles (Na), polymorphism information content (PIC), and Shannon’s index (I) were assessed using GenAlEx 6.5 (Peakall & Smouse, 2012) and Microsatellite Tools (Trinity College Dublin, Ireland). Observed heterogeneity (HO), expected heterozygosity (HE), and inbreeding coefficient (FIS) were estimated using the *hierfstat *R package (Goudet, 2005). The analysis of molecular variance (AMOVA) was calculated by ARLEQUIN 3.5 (Excoffier & Lischer, 2010) to assess the proportional distribution of genetic variation among populations, individuals, and groups, and to estimate the fixed coefficient between populations (FST). The genetic structure of populations was analyzed based on Bayesian clustering and phylogenetic tree, using STRUCTURE 2.3.4 (Pritchard et al., 2000) and MEGA 6.0 (Tamura et al., 2013), respectively.