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This dataset reports the raw data collected during an experiment where hosts (mice) were infected with two parasites [the nematode Heligmosomoides polygyrus (Hp) and the protozoan Plasmodium yoelii (Py)]. In the experiment, hosts were infected according to different orders and timings of infection with the aim of exploring the effect of coinfection on the dynamics of Hp infection. During the experiment, we collected variables referring to Hp infection dynamics (number of eggs excreted, worm dry biomass and within-host persistence) and host immune response (gene expression of the Th2 cytokines IL-4 and IL-13, level of the Th2 cytokines IL-5 and IL-13 in plasma, proportion of GATA-3+ cells among CD4+ T lymphocytes in the spleen).

In addition, we ran a second experiment where hosts (mice) infected with Hp where treated with recombinant IL-13, anti-IL-13 monoclonal antibodies, or with IgG1 with the aim of investigating if the manipulation of the Th2 response can reproduce the changes in Hp fecundity observed in coinfected hosts.

The data have been organized in separate .xlsx files, one file per variable. For instance, the file named "1_data on fecal egg count" reports the data on fecal egg count in the different experimental groups.

Data files have been numbered according to the order of appearance in the result section of the corresponding article.

We also provide the code files that have been used to analyse the data and produce the figures. These .txt files have been numbered as for the data files, so the file "1_code for the analysis of fec" corresponds to the code used to analyse the data reported in the file "1_data on fecal egg count".

Description of the data and file structure

Here we describe the variables in each data file (with the associated code file)

1_data on fecal egg count (associated to 1_code for the analysis of fec)

• mouse_id : the identity of the mouse
• replicate : the identity of the replicated dataset (1 or 2)
• time_post_infection : the time post Hp infection in days
• fec : number of Hp eggs per 200 mg of feces
• Treatment :
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• lfec : ln-transformed Hp eggs per gram of feces [ln(fec*5)]

2_data on worm mass (associated to 2_code for the analysis of worm mass)

• mouse_id : the identity of the mouse
• replicate : the identity of the replicated dataset (1 or 2)
• time_post_infection : the time post Hp infection in days
• worm_mass : dry mass of adult worms retreived from the intestinal lumen (mg)
• Treatment :
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus

3_data on fecal egg count pre and post Py infection (associated to 3_code for the analysis of fec pre and post Py infection).

This file contains two worksheets. Worksheet "pre- post-Py" refers to data gathered in coinfected hosts pre- and post-Py infection. Worksheet "pre- post-day 28" refers to data gathered in single Hp infected hosts pre- and post-day 28 post-Hp infection

• mouse_id : the identity of the mouse
• time_post_infection : the time post Hp infection in days
• fec : number of Hp eggs per 200 mg of fèces
• lfec : ln-transformed number of Hp eggs per gram of feces [ln(fec*5)]
• Treatment :
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• Timing: pre Py infection = period preceeding Py infection in the Hp-28+Py group (in worksheet "pre- post-Py")
  •   post Py infection = period succeeding Py infection in the Hp-28+Py group (in worksheet "pre- post-Py")
  •   pre day 28 pi = period prior to day 28 post Hp infection in the single Hp group (in worksheet "pre- post-day 28")
  •   post day 28 pi = period post to day 28 post Hp infection in the single Hp group (in worksheet "pre- post-day 28")

4_data on the expression of IL4 and IL13 genes (associated to 4_code for the analysis of the expression of il4 gene and 4_code for the analysis of the expression of il13 gene)

• mouse_id : the identity of the mouse
• replicate : the identity of the replicated dataset (1 or 2)
• time_post_infection : the time post Hp infection in days
• Treatment :
  • non-infected = control individuals
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• delta_ct_il4 : delta Ct of IL-4 gene with respect to beta-actin
• delta_ct_il13 : delta Ct of IL-13 gene with respect to beta-actin (for one sample the amplification failed explaining the missing value = empty cell)
• fold_change_il4 : fold change of IL-4 gene with respect to non-infected hosts [therefore, for non-infected hosts (the reference group), the cells are empty]
• fold_change_il13 : fold change of IL-13 gene with respect to non-infected hosts [therefore, for non-infected hosts (the reference group), the cells are empty]

5_data on IL5 (associated to 5_code for the analysis of IL5 in plasma)

• mouse_id : the identity of the mouse
• replicate : the identity of the replicated dataset (1 or 2)
• time_post_infection : the time post Hp infection in days
• Treatment :
  • non-infected = control individuals
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• IL5 : level of il5 in plasma (pg/ml)

6_data on IL13 (associated to 6_code for the analysis of IL13 in plasma)

• mouse_id : the identity of the mouse
• replicate : the identity of the replicated dataset (1 or 2)
• time_post_infection : the time post Hp infection in days
• Treatment :
  • non-infected = control individuals
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• il13 : level of il13 in plasma (pg/ml)

7_data on th2 (associated to 7_code for the analysis of th2 cells)

• mouse_id : the identity of the mouse
• replicate : the identity of the replicated dataset (1 or 2)
• time_post_infection : the time post Hp infection in days
• Treatment :
  • non-infected = control individuals
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• CD4_T_cells : number of CD4+ T cells
• number_Th2 : number of Th2 cells
• th2 : proportion of Th2 among CD4+ T cells

8_data on the manipulation of IL13 (associated to 8_code for the analysis of fec in the experiment on IL13)

• mouse_id : the identity of the mouse
• time_post_infection : the time post Hp infection in days
• Treatment :
  • il13 = administration of recombinant IL-13
  • anti_il13 = administration of anti IL-13 antibodies
  • igg = administration of IgG1
• lfec = ln-transformed Hp eggs per gram of fèces

9_data on cumulative egg excretion (associated to 9_code for the analysis of cumulative egg excretion).

This file contains two worksheets. Worksheet "single Hp vs coinf when Py 1st" refers to data gathered in single Hp infected hosts and hosts that were first infected with Py and then with Hp. Worksheet "single Hp vs coinf when Hp 1st" refers to data gathered in single Hp infected hosts and hosts that were first infected with Hp and then with Py

• LRS : cumulative number of eggs excreted up to day 99 p.i. relative to the single Hp infection group
• Treatment : single Hp = single infection with Heligmosomoides polygyrus
• Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus (in worksheet "single Hp vs coinf when Py 1st")
• Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus (in worksheet "single Hp vs coinf when Py 1st")
• Hp-28+Py = first infection with Heligmosomoides polygyrus and after 28 days second infection with Plasmodium yoelii (in worksheet "single Hp vs coinf when Hp 1st")

10_data on Hp persistence (associated to 10_code for the analysis of Hp persistence)

• clearance : the time in days up to Hp infection clearence
• censor : 0 = censored events (i.e., cases where Hp infection was still present at day 99 pi)
• Treatment :
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
  • Hp-28+Py = first infection with Heligmosomoides polygyrus and after 28 days second infection with Plasmodium yoelii

11_data on mean ct IL4 IL13 (associated to 11_code for the analysis of mean ct il4 il13 over time)

• time_post_infection : the time post Hp infection in days
• Treatment :
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• mean_ct_il4 : mean Ct (over two technical replicates) of IL-4 gene
• mean_ct_il13 : mean Ct (over two technical replicates) of IL-13 gene (for one sample the amplification failed explaining the missing value = empty cell)

12_data on delta ct and fold change IL4 and IL13 at day 99 pi (associated to 12_code for the analysis of delta ct at day 99 pi)

• time_post_infection : the time post Hp infection in days
• Treatment :
  • single Hp = single infection with Heligmosomoides polygyrus
  • Py-14+Hp = first infection with Plasmodium yoelii and after 14 days second infection with Heligmosomoides polygyrus
  • Py-28+Hp = first infection with Plasmodium yoelii and after 28 days second infection with Heligmosomoides polygyrus
• delta_ct_il4 : delta ct of IL-4 gene with respect to beta-actin
• delta_ct_il13 : delta ct of IL-13 gene with respect to beta-actin
• fold_change_il4 : fold change of IL-4 gene with respect to the single Hp infected group [therefore, for single Hp infected hosts (the reference group), the cells are empty]
• fold_change_il13 : fold change of IL-13 gene with respect to the single Hp infected group [therefore, for single Hp infected hosts (the reference group), the cells are empty]

Code/Software

Data files are .xlsx that can be read with excel.
Code files are .txt that can be read with SAS Studio (the software used to analyse the data and draw the figures).