Convergent genomic signatures of local adaptation across a continental-scale environmental gradient
Data files
Mar 08, 2023 version files 1.24 GB
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D.pubescens_AK_all_chr_Hscan.rol_win
25.36 MB
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D.pubescens_candidates_windows_PC1P.csv
5.28 KB
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D.pubescens_candidates_windows_PC1T.csv
10.04 KB
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D.pubescens_candidates_windows_PC2P.csv
1.31 KB
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D.pubescens_candidates_windows_PC2T.csv
1.51 KB
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D.pubescens_candidates_windows_PC3P.csv
3.99 KB
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D.pubescens_candidates_windows_PC3T.csv
313 B
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D.pubescens_E_all_chr_Hscan.rol_win
25.51 MB
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D.pubescens_FST_comparisons.zip
169.82 MB
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D.pubescens_NW_all_chr_Hscan.rol_win
25.36 MB
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D.pubescens_PC1P.permutation.rol_win
26.24 MB
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D.pubescens_PC1P.rol_win
26.24 MB
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D.pubescens_PC1T.permutation.rol_win
26.23 MB
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D.pubescens_PC1T.rol_win
26.24 MB
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D.pubescens_PC2P.permutation.rol_win
26.24 MB
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D.pubescens_PC2P.rol_win
26.25 MB
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D.pubescens_PC2T.permutation.rol_win
26.23 MB
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D.pubescens_PC2T.rol_win
26.25 MB
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D.pubescens_PC3P.permutation.rol_win
26.23 MB
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D.pubescens_PC3P.rol_win
26.24 MB
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D.pubescens_PC3T.permutation.rol_win
26.24 MB
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D.pubescens_PC3T.rol_win
26.25 MB
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D.pubescens_PCAdapt.candidates.tsv
64.30 KB
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D.pubescens_PCAdapt.rol_win
32.31 MB
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D.pubescens_R_all_chr_Hscan.rol_win
25.49 MB
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D.pubescens_T.guttata-pseudochromosomes.cds.gff
10.49 MB
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D.villosus_AK_all_chr_Hscan.rol_win
24.98 MB
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D.villosus_candidates_windows_PC1P.csv
24.77 KB
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D.villosus_candidates_windows_PC1T.csv
11.18 KB
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D.villosus_candidates_windows_PC2P.csv
18.03 KB
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D.villosus_candidates_windows_PC2T.csv
52.91 KB
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D.villosus_candidates_windows_PC3P.csv
27.06 KB
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D.villosus_candidates_windows_PC3T.csv
47.28 KB
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D.villosus_E_all_chr_Hscan.rol_win
25.11 MB
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D.villosus_FST_comparisons.zip
170.88 MB
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D.villosus_NW_all_chr_Hscan.rol_win
24.99 MB
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D.villosus_PC1P.permutation.rol_win
25.85 MB
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D.villosus_PC1P.rol_win
25.86 MB
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D.villosus_PC1T.permutation.rol_win
25.85 MB
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D.villosus_PC1T.rol_win
25.86 MB
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D.villosus_PC2P.permutation.rol_win
25.86 MB
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D.villosus_PC2P.rol_win
25.86 MB
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D.villosus_PC2T.permutation.rol_win
25.85 MB
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D.villosus_PC2T.rol_win
25.86 MB
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D.villosus_PC3P.permutation.rol_win
25.85 MB
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D.villosus_PC3P.rol_win
25.86 MB
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D.villosus_PC3T.permutation.rol_win
25.85 MB
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D.villosus_PC3T.rol_win
25.86 MB
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D.villosus_PCAdapt.candidates.tsv
156.60 KB
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D.villosus_PCAdapt.rol_win
31.91 MB
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D.villosus_R_all_chr_Hscan.rol_win
25.14 MB
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README.md
9.58 KB
Abstract
Convergent local adaptation offers a glimpse into the role of constraint and stochasticity in adaptive evolution, in particular the extent to which similar genetic mechanisms drive adaptation to common selective forces. Here, we investigated the genomics of local adaptation in two non-sister woodpeckers that are co-distributed across an entire continent and exhibit remarkably convergent patterns of geographic variation. We sequenced the genomes of 140 individuals of Downy (Dryobates pubescens) and Hairy (D. villosus) woodpeckers and employed a suite of genomic approaches to identify loci under selection. We showed evidence that convergent genes have been targeted by selection in response to shared environmental pressures, such as temperature and precipitation. Among candidates, we found multiple genes putatively linked to key phenotypic adaptations to climate, including differences in body size (e.g., IGFPB) and plumage (e.g., MREG). These results are consistent with genetic constraints limiting the pathways of adaptation to broad climatic gradients, even after genetic backgrounds diverge.
Whole genome sequencing of 140 samples of Downy (Dryobates pubescens) and Hairy (D. villosus) Woodpecker from seven (n per population = 10) North American populations.
D.pubescens_T.guttata-pseudochromosomes.cds.gff - Gene annotation of Downy Woodpecker mapped to the pseudochromosome reference genome.
D.pubescens_candidates_windows_PC1T.csv - GEA significant windows for PC1 of temperature in D. pubescens
D.pubescens_candidates_windows_PC2T.csv - GEA significant windows for PC2 of temperature in D. pubescens
D.pubescens_candidates_windows_PC3T.csv - GEA significant windows for PC3 of temperature in D. pubescens
D.pubescens_candidates_windows_PC1T.csv - GEA significant windows for PC1 of precipitation in D. pubescens
D.pubescens_candidates_windows_PC2P.csv - GEA significant windows for PC2 of precipitation in D. pubescens
D.pubescens_candidates_windows_PC3P.csv - GEA significant windows for PC3 of precipitation in D. pubescens
D.pubescens_PC1T.rol_win - Per-window median LRT scores for the association with PC1 of temperature in D. pubescens
D.pubescens_PC2T.rol_win - Per-window median LRT scores for the association with PC2 of temperature in D. pubescens
D.pubescens_PC3T.rol_win - Per-window median LRT scores for the association with PC3 of temperature in D. pubescens
D.pubescens_PC1T.rol_win - Per-window median LRT scores for the association with PC1 of precipitation in D. pubescens
D.pubescens_PC2P.rol_win - Per-window median LRT scores for the association with PC2 of precipitation in D. pubescens
D.pubescens_PC3P.rol_win - Per-window median LRT scores for the association with PC3 of precipitation in D. pubescens
D.pubescens_PC1T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC1 of temperature in D. pubescens
D.pubescens_PC2T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC2 of temperature in D. pubescens
D.pubescens_PC3T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC3 of temperature in D. pubescens
D.pubescens_PC1T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC1 of precipitation in D. pubescens
D.pubescens_PC2P.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC2 of precipitation in D. pubescens
D.pubescens_PC3P.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC3 of precipitation in D. pubescens
D.villosus_candidates_windows_PC1T.csv - GEA significant windows for PC1 of temperature in D. villosus
D.villosus_candidates_windows_PC2T.csv - GEA significant windows for PC2 of temperature in D. villosus
D.villosus_candidates_windows_PC3T.csv - GEA significant windows for PC3 of temperature in D. villosus
D.villosus_candidates_windows_PC1T.csv - GEA significant windows for PC1 of precipitation in D. villosus
D.villosus_candidates_windows_PC2P.csv - GEA significant windows for PC2 of precipitation in D. villosus
D.villosus_candidates_windows_PC3P.csv - GEA significant windows for PC3 of precipitation in D. villosus
D.villosus_PC1T.rol_win - Per-window median LRT scores for the association with PC1 of temperature in D. villosus
D.villosus_PC2T.rol_win - Per-window median LRT scores for the association with PC2 of temperature in D. villosus
D.villosus_PC3T.rol_win - Per-window median LRT scores for the association with PC3 of temperature in D. villosus
D.villosus_PC1T.rol_win - Per-window median LRT scores for the association with PC1 of precipitation in D. villosus
D.villosus_PC2P.rol_win - Per-window median LRT scores for the association with PC2 of precipitation in D. villosus
D.villosus_PC3P.rol_win - Per-window median LRT scores for the association with PC3 of precipitation in D. villosus
D.villosus_PC1T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC1 of temperature in D. villosus
D.villosus_PC2T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC2 of temperature in D. villosus
D.villosus_PC3T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC3 of temperature in D. villosus
D.villosus_PC1T.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC1 of precipitation in D. villosus
D.villosus_PC2P.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC2 of precipitation in D. villosus
D.villosus_PC3P.permutation.rol_win - Per-window median LRT scores for 200 random permutations of the association with PC3 of precipitation in D. villosus
D.pubescens_FST_comparisons.zip:
PP-<pop1>-<pop2>.50kb-10kbstep.pdf - Manhattan plot of Fst values estimated for 50kb windows (10 kb increments) comparing <pop1> and <pop2> in D. pubescens
PP-<pop1>-<pop2>.FST_histogram.pdf - Histogram of Fst values estimated for 50kb windows (10 kb increments) comparing <pop1> and <pop2> in D. pubescens
PP-<pop1>-<pop2>.fst_slidingwindow - Fst values estimated for 50kb windows (10 kb increments) comparing <pop1> and <pop2> in D. pubescens
PP-<pop1>-<pop2>.outliers_5sd.csv - Outlier Fst windows (>5 standard deviations) for the comparison between <pop1> and <pop2> in D. pubescens
PP-<pop1>-<pop2>-candidates.csv - Candidate genes within outlier Fst windows (>5 standard deviations) for the comparison between <pop1> and <pop2> in D. pubescens
D.villosus_FST_comparisons.zip:
PV-<pop1>-<pop2>.50kb-10kbstep.pdf - Manhattan plot of Fst values estimated for 50kb windows (10 kb increments) comparing <pop1> and <pop2> in D. villosus
PV-<pop1>-<pop2>.FST_histogram.pdf - Histogram of Fst values estimated for 50kb windows (10 kb increments) comparing <pop1> and <pop2> in D. villosus
PV-<pop1>-<pop2>.fst_slidingwindow - Fst values estimated for 50kb windows (10 kb increments) comparing <pop1> and <pop2> in D. villosus
PV-<pop1>-<pop2>.outliers_5sd.csv - Outlier Fst windows (>5 standard deviations) for the comparison between <pop1> and <pop2> in D. villosus
PV-<pop1>-<pop2>-candidates.csv - Candidate genes within outlier Fst windows (>5 standard deviations) for the comparison between <pop1> and <pop2> in D. villosus
D.pubescens_AK_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the Alaska population of D. pubescens
D.pubescens_E_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the East population of D. pubescens
D.pubescens_R_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the Rockies population of D. pubescens
D.pubescens_NW_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the Northwest population of D. pubescens
D.villosus_AK_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the Alaska population of D. villosus
D.villosus_E_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the East population of D. villosus
D.villosus_R_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the Rockies population of D. villosus
D.villosus_NW_all_chr_Hscan.rol_win - Median H scores for 50 SNP windows (10 SNP increments) in the Northwest population of D. villosus
D.pubescens_PCAdapt.candidates.tsv - Candidate SNP windows for the PCAdapt analysis in D. pubescens
D.pubescens_PCAdapt.rol_win - Median p-values of each 50 SNP windows (10 SNP increments) for the PCAdapt analysis in D. pubescens
D.villosus_PCAdapt.candidates.tsv - Candidate SNP windows for the PCAdapt analysis in D. villosus
D.villosus_PCAdapt.rol_win - Median p-values of each 50 SNP windows (10 SNP increments) for the PCAdapt analysis in D. villosus