Overview

This dataset contains fluorescence microscopy videos of DNA origami nanosprings used to measure the force generated by the kinesin-3 motor protein KIF1A. The dataset includes recordings for wild-type (WT), homozygous mutants (P305L/P305L, V8M/V8M, A255V/A255V), and heterozygous mutants (P305L/WT, V8M/WT, A255V/WT).

Each video captures nanospring extension under force, enabling analysis of motor-driven movement and estimation of stall force.

File Structure

The dataset is organized into directories by construct:

WT_WT/ : Wild-type KIF1A
P305L_P305L/ : Homozygous mutant (P305L/P305L)
V8M_V8M/ : Homozygous mutant (V8M/V8M)
A255V_A255V/ : Homozygous mutant (A255V/A255V)
P305L_WT/ : Heterozygous mutant (P305L/WT)
V8M_WT/ : Heterozygous mutant (V8M/WT)
A255V_WT/ : Heterozygous mutant (A255V/WT)

Each directory contains multiple .avi video files corresponding to independent experimental recordings.

File Description

• Each .avi file represents a fluorescence microscopy recording of a single nanospring experiment.
• Videos contain time-lapse image sequences of fluorescently labeled nanosprings.
• No preprocessing (e.g., filtering or background subtraction) has been applied.

Experimental Details

• System: DNA origami nanospring assay
• Motor protein: KIF1A (wild-type and mutants)
• Imaging method: Fluorescence microscopy
• Fluorophore: Cy3
• Frame rate: 33 frames per second
• Pixel size: 65 nm/pixel

Data Format

• File format: AVI
• Encoding: unknown
• Each frame corresponds to a single time point in the experiment

Software Requirements

The data can be analyzed using:

• Python
• ImageJ / Fiji