Data from: Paternity analysis, pollen flow, and spatial genetic structure of a natural population of Euterpe precatoria in the Brazilian Amazon
Data files
Jun 04, 2026 version files 50.42 KB
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Epr_Fluxo_Data_Dryad.txt
46.01 KB
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README.md
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Abstract
Euterpe precatoria, known as açaí do Amazonas, is a regionally important palm of the Amazon rainforest for the fruit production through extractive agriculture. Little information is available with regard to genetic diversity, gene flow, and spatial genetic structure (SGS) of açaí populations, which are essential for the use, management, and conservation of genetic resources of the species. This research aimed to assess the genetic diversity, inbreeding level, SGS, and gene flow in four ontogenetic stages of a natural E. precatoriapopulation in the Brazilian Amazon, based on 18 microsatellite loci. The study was carried out in a natural population dispersed in an area of about 10 ha. Leaf tissues of 248 plants were mapped and sampled, and classified into four ontogenetic stages: reproductive (59), immature (70), young (60), and seedling (59). Genetic diversity indices were high for all ontogenetic stages. The fixation index (F) for all ontogenetic stages was not significantly different from zero, indicating the absence of inbreeding. A significant SGS was found for all ontogenetic stages (68–110 m), indicating seed dispersal over short distances. Paternity analysis detected pollen immigration of 39.1%, a selfing rate of 4.2%, and a mean pollen dispersal distance within the population of 531 m. The results indicate substantial allele input in the population via pollen immigration, contributing to the maintenance of the genetic diversity of the population. However, within a population, the renewal with new progenies selected from seed plants spaced at least 110 m apart is important to avoid collecting seeds from related plants.
Dataset DOI: 10.5061/dryad.7s7f7
Description of the data and file structure
Data from: Paternity analysis, pollen flow and spatial genetic structure of a natural population of Euterpe precatoria in the Brazilian Amazon
Authors
Santiago Linorio Ferreyra Ramos, Gabriel Dequigiovanni, Alexandre Magno Sebbenn, Maria Teresa Gomes Lopes, Jeferson Luis Vasconcelos de Macêdo, Elizabeth Ann Veasey, Alessandro Alves-Pereira, Perla Pimentel da Silva, José Nivaldo Garcia, and Paulo Yoshio Kageyama.
Associated Publication
Ramos, S. L. F., Dequigiovanni, G., Sebbenn, A. M., Lopes, M. T. G., Macêdo, J. L. V., Veasey, E. A., Alves-Pereira, A., Silva, P. P., Garcia, J. N., & Kageyama, P. Y. (2018). Paternity analysis, pollen flow, and spatial genetic structure of a natural population of Euterpe precatoria in the Brazilian Amazon. Ecology and Evolution, 8, 11143–11157. https://doi.org/10.1002/ece3.4582
Study Description
This dataset was generated to investigate paternity patterns, pollen flow, spatial genetic structure (SGS), genetic diversity, and inbreeding levels in a natural population of Euterpe precatoria Mart. (Amazonian açaí palm) in the Brazilian Amazon.
Leaf tissue samples were collected from individuals in a natural forest population located in Nossa Senhora de Fátima do Açaí, Vila Amazônia, municipality of Parintins, Amazonas, Brazil. Individuals were georeferenced and classified into four ontogenetic stages: reproductive, immature, young, and seedling.
DNA was extracted from leaf tissues and individuals were genotyped using 18 species-specific microsatellite (SSR) loci developed for Euterpe precatoria. Genetic analyses included genetic diversity, fixation index, Bayesian population structure, spatial genetic structure (SGS), paternity assignment, pollen flow, and seed dispersal analyses.
Study Site
Location: Nossa Senhora de Fátima do Açaí, Vila Amazônia, Parintins, Amazonas, Brazil.
Coordinates:
Latitude: 02°36'52.09"S
Longitude: 56°33'29.13"W
Biome: Amazon Rainforest
Files and variables
File: Epr_Fluxo_Data_Dryad.txt
Description:
Variables
- Ontogenetic stages:
- N Plant:
- UTM-Easting:
- UTM-Northing:
- Height plant:
Sampling Design
A total of 248 individuals were sampled in approximately 10 hectares of natural forest.
Ontogenetic stages:
- Reproductive plants: 59 individuals
- Immature plants: 70 individuals
- Young plants: 60 individuals
- Seedlings: 59 individuals
Each sampled individual was georeferenced using GPS and leaf tissue samples were collected for DNA extraction.
Molecular Markers
Eighteen microsatellite (SSR) loci were used:
Epr01, Epr02, Epr04, Epr05, Epr06, Epr13, Epr14, Epr15, Epr18, Epr19, Epr21, Epr22, Epr27, Epr31, Epr32, Epr33, Epr35, and Epr36.
File Descriptions
The dataset may contain the following file types:
Genotype Data
Microsatellite genotype matrix of sampled individuals.
Columns may include:
- Individual ID
- Ontogenetic stage
- Geographic coordinates (latitude/longitude)
- Microsatellite loci genotypes
Genotypes are represented as allele sizes (bp).
Spatial Data
Geographic coordinates of sampled individuals used in spatial genetic structure and gene flow analyses.
Paternity Analysis
Parentage assignment outputs used to estimate pollen immigration, selfing rate, and pollen dispersal distance.
Abbreviations
SSR = Simple Sequence Repeat (microsatellite marker)
SGS = Spatial Genetic Structure
Ho = Observed heterozygosity
He = Expected heterozygosity
F = Fixation index
Ne = Effective population size
Pp = Probability of exclusion of parent pairs
mp = Pollen immigration rate
δp = Pollen dispersal distance
δs = Seed dispersal distance
Software and Analysis Tools
The following software may be required to interpret or reproduce analyses:
- CERVUS 3.0.3 (paternity analysis)
- SPAGeDI 1.3 (spatial genetic structure)
- STRUCTURE (Bayesian clustering)
- CLUMPP v1.1.2
- Distruct v1.1
- R statistical environment
- GENEMAPPER v4.0
- NM+ (Neighborhood Model)
Access information
Other publicly accessible locations of the data:
- None
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