Biophysical properties governing septin assembly
Data files
Apr 20, 2021 version files 201.92 GB
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1-AGY581_dilute_combine_bending_modes.txt
3.03 KB
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1-AGY581_dilute_combine_contours.txt
1.48 MB
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1-AGY581_dilute_combine_Cs_raw.txt
1.88 GB
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1-AGY581_dilute_combine_end_to_end_vs_contour_lengths.txt
434 B
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1-AGY581_dilute_combine_resized_ave_Cs.txt
14.34 KB
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1-AGY581_dilute_combine_resized_bending_modes.txt
3.29 KB
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1-AGY581_dilute_combine_resized_contours.txt
146.33 KB
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1-AGY581_dilute_combine_resized_Cs_raw.txt
19.05 MB
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1-AGY581_dilute_combine_resized_end_to_end_vs_contour_lengths.txt
406 B
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1-AGY581_dilute_combine_resized_select.mat
37.36 KB
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1-AGY581_dilute_combine_resized_tangent_angles.txt
149.14 KB
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1-AGY581_dilute_combine_select.mat
275.73 KB
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1-AGY581_dilute_combine_skeleton_resized_ave_Cs.txt
14.34 KB
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1-AGY581_dilute_combine_skeleton_resized_bending_modes.txt
3.29 KB
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1-AGY581_dilute_combine_skeleton_resized_contours.txt
146.33 KB
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1-AGY581_dilute_combine_skeleton_resized_Cs_raw.txt
19.05 MB
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1-AGY581_dilute_combine_skeleton_resized_end_to_end_vs_contour_lengths.txt
406 B
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1-AGY581_dilute_combine_skeleton_resized_select.mat
37.36 KB
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1-AGY581_dilute_combine_skeleton_resized_tangent_angles.txt
149.14 KB
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1-AGY581_dilute_combine_tangent_angles.txt
1.60 MB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_ave_Cs.txt
39.61 KB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_bending_modes.txt
9.25 KB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_contours.txt
1.02 MB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_Cs_raw.txt
291.25 MB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_ave_Cs.txt
39.68 KB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_bending_modes.txt
6.08 KB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_contours.txt
862.45 KB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_Cs_raw.txt
275.32 MB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_end_to_end_vs_contour_lengths.txt
716 B
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_select.mat
180.65 KB
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A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton_tangent_angles.txt
868.47 KB
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AGY581_1-500th_3_hours_1st_cs_033.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_034.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_035.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_036.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_037.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_038.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_039.nd2
29.01 MB
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AGY581_1-500th_3_hours_1st_cs_040.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_022.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_023.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_024.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_025.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_026.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_027.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_028.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_029.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_030.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_031.nd2
29.01 MB
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AGY581_1-500th_3_hours_2nd_cs_032.nd2
29.01 MB
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AGY581_tenth_dilution_037-1_ave_Cs.txt
5.81 KB
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AGY581_tenth_dilution_037-1_bending_modes.txt
209.56 KB
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AGY581_tenth_dilution_037-1_contours.txt
2.28 MB
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AGY581_tenth_dilution_037-1_Cs_raw.txt
50.06 MB
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AGY581_tenth_dilution_037-1_end_to_end_vs_contour_lengths.txt
25.11 KB
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AGY581_tenth_dilution_037-1_select.mat
640.95 KB
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AGY581_tenth_dilution_037-1_tangent_angles.txt
2.29 MB
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bwp029_300_pM_length_at_SS_006.nd2
29.01 MB
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bwp029_300_pM_length_at_SS_007.nd2
29.01 MB
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bwp029_300_pM_length_at_SS_008.nd2
29.01 MB
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bwp029_300_pM_length_at_SS_009.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_015.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_016.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_017.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_018.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_019.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_020.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_021.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_022.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_023.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_024.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_025.nd2
29.01 MB
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BWX033_AGY581_one_hundreth_dilution_026.nd2
29.01 MB
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bwx101_300_pM_lengths.txt
50.40 KB
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bwx102_300_pM_lengths.txt
84.93 KB
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bwx103_300_pM_lengths.txt
21.82 KB
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BWX105_-_wt-SNAP488_1_nM_time_lapse_001_large_image.nd2
48.72 MB
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bwx106_400_pM_lengths.txt
74.10 KB
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bwx110_100_pM_cs2_lengths.txt
128.40 KB
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bwx111_100_pM_cs1_lengths.txt
81.52 KB
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DENSITY_HIGH_024.tif
2.94 MB
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DENSITY_HIGH_025.tif
2.94 MB
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DENSITY_HIGH_026.tif
2.94 MB
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DENSITY_HIGH_027.tif
2.94 MB
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DENSITY_HIGH_028.tif
2.94 MB
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DENSITY_LOW_001.tif
2.94 MB
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DENSITY_LOW_002.tif
2.94 MB
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DENSITY_LOW_003.tif
2.94 MB
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DENSITY_LOW_004.tif
2.94 MB
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DENSITY_LOW_005.tif
2.94 MB
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DENSITY_MEDIUM_014.tif
2.94 MB
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DENSITY_MEDIUM_015.tif
2.94 MB
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DENSITY_MEDIUM_017.tif
2.94 MB
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DENSITY_MEDIUM_018.tif
2.94 MB
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DENSITY_MEDIUM_019.tif
2.94 MB
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extract_1-100th_2nd_cs_117.nd2
29.01 MB
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extract_1-100th_2nd_cs_118.nd2
29.01 MB
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extract_1-100th_2nd_cs_119.nd2
29.01 MB
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extract_1-100th_2nd_cs_120.nd2
29.01 MB
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extract_1-100th_2nd_cs_121.nd2
29.01 MB
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extract_1-100th_2nd_cs_124.nd2
29.01 MB
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Fig_1E_-_BWX074_10_pM_80ms_027-2.tif
4.38 MB
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Fig_1F_-_cdc11alpha6_20_pM_010.nd2
14.57 MB
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Fig_1F_-_cdc11alpha6_20_pM_011.nd2
14.57 MB
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Fig_1F_-_cdc11alpha6_20_pM_012.nd2
14.57 MB
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Fig_1F_-_cdc11alpha6_20_pM_013.nd2
14.57 MB
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Fig_1F_-_wt_10_pM_80ms_001.nd2
14.57 MB
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Fig_1F_-_wt_10_pM_80ms_002.nd2
14.57 MB
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Fig_1F_-_wt_10_pM_80ms_003.nd2
14.57 MB
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Fig_1F_-_wt_10_pM_80ms_004.nd2
14.57 MB
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Fig_1F_and_5E_-_bwx119_octamer_fluorescence.xlsx
1.80 MB
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Fig_1G_-_bwx111_filament_fluorescence.xlsx
388.16 KB
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Fig_1G_-_bwx111_wt_100_pM_1st_cs_033.nd2
14.57 MB
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Fig_1H_-_BWX074_fluorescence_intensity_converted_to_lengths.txt
66.97 KB
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Fig_1I_-_bwx124_200_pM_adsorbance_first_20_min.xlsx
24.45 KB
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Fig_1I_-_wt488_200_pM_20_min_time_lapse_001.nd2
60.97 MB
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Fig_1I_-_wt488_200_pM_20_min_time_lapse_002.nd2
60.97 MB
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Fig_1I_-_wt488_200_pM_20_min_time_lapse_003.nd2
60.97 MB
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Fig_1J_-_BWX100_bwp029_300_pM_final.nd2
523.01 MB
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Fig_2B_-_bwx110_wt_100_pM_2nd_cs_013-1.tif
242.15 KB
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Fig_2B_-_bwx110_wt_2_nM_1st_cs_067-1.tif
242.12 KB
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Fig_2C_-_bwx101_bwp029_300_pM_time_lapse_only_001.nd2
525.09 MB
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Fig_2C_-_BWX101-103_saturation_curve.xlsx
26 KB
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Fig_2C_-_bwx102_wt-SNAP488_300_pM_time_lapse_001.nd2
242.89 MB
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Fig_2C_-_bwx103_wt-SNAP488_300_pM_time_lapse_001-5.nd2
524.71 MB
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Fig_2D_-_bwx102_300_pM_lengths.txt
84.93 KB
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Fig_2D-_bwx102_wt-SNAP488_300_pM_003.nd2
29.01 MB
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Fig_2D-_bwx102_wt-SNAP488_300_pM_004.nd2
29.01 MB
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Fig_2D-_bwx102_wt-SNAP488_300_pM_008.nd2
29.01 MB
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Fig_2E_-_bwx092_wt_100_nM_33_min_031.nd2
29.01 MB
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Fig_2E_-_bwx092_wt_100_nM_33_min_032.nd2
29.01 MB
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Fig_2E_-_BWX092_wt_100_pM_33_min.txt
5.97 KB
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Fig_3A_Table_1_-_bwx109_50_pM_11alpha6_octamer_dwell_times.txt
138.96 KB
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Fig_3A_Table_1_-_bwx109_cdc11alpha6_50_pM_004.nd2
288.90 MB
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Fig_3A_Table_1_-_bwx109_cdc11alpha6_50_pM_005.nd2
288.90 MB
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Fig_3A_Table_1_-_bwx109_cdc11alpha6_50_pM_006.nd2
288.90 MB
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Fig_3A_Table_1_-_bwx109_Kon_Koff.xlsx
10.33 MB
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Fig_3A_Table_1_-_bwx110_50_pM_11alpha6_octamer_duration_times.txt
101.25 KB
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Fig_3A_Table_1_-_bwx110_cdc11alpha6_septin-membrane_Kd.xlsx
3.23 MB
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Fig_3A_Table_1_-_bwx115_20_pM_11alpha6_octamer_duration_times.txt
119.93 KB
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Fig_3A_Table_1_-_bwx115_cdc11alpha6_Koff_calc.xlsx
5.21 MB
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Fig_3A_Table_1_-_bwx117_20_pM_kon_koff_calc.xlsx
6.82 MB
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Fig_3A_Table_1_-bwx117_20_pM_octamer_dwell_times.txt
106.29 KB
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Fig_3A&D_Table_1_-_bwx110_cdc11alpha6_50_pM_001.nd2
577.68 MB
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Fig_3A&D_Table_1_-_bwx110_cdc11alpha6_50_pM_003.nd2
577.68 MB
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Fig_3A&D_Table_1_-_bwx115_11alpha6_20_pM_006.nd2
577.68 MB
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Fig_3A&D_Table_1_-_bwx115_11alpha6_20_pM_008.nd2
577.68 MB
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Fig_3A&D_Table_1_-_bwx115_11alpha6_20_pM_009.nd2
577.68 MB
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Fig_3A&D_Table_1_-_bwx115_11alpha6_20_pM_010.nd2
577.68 MB
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Fig_3A&D._Table_1_-_bwx110_cdc11alpha6_50_pM_002.nd2
577.68 MB
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Fig_3BC_-_septin_membrane_density_calc.xlsx
44.70 KB
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Fig_3D_Table_1_-_bwx110_cdc11alpha6_XYt_spot_tracking.xlsx
54.39 MB
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Fig_3D_Table_1_-_bwx115_cdc11alpha6_tracking_spot_XYt_coordinates.xlsx
3.73 MB
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Fig_3D_Table_1_-_bwx117_20_pM_11alpha6_spot_tracking_XY_coordinates.xlsx
134.07 MB
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Fig_3E_6E_-_bwx100_wt-SNAP488_300_pM_montage.tif
8.64 MB
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Fig_4F_-_simulation_analyses.xlsx
68.16 KB
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Fig_5A_-_bwx114_-_AGY581_1-10th_dilution_3.5_hrs_019.nd2
14.57 MB
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Fig_5A_-_bwx114_-_AGY581_undiluted_3.5_hrs_025.nd2
14.57 MB
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Fig_5A_-_bwx116_-_AGY581_1-200th_3_hours_2nd_cs_018.nd2
29.01 MB
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Fig_5B_-_bwx125_agy581_1-200th_20_min_time_lapse_001.nd2
61.06 MB
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Fig_5B_-_bwx125_agy581_1-200th_20_min_time_lapse_004.nd2
61.06 MB
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Fig_5B_-_bwx125_AGY581_wt_extract_short_timelapse_adsorbance_quantification.xlsx
20.26 KB
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Fig_5C_&_D_-_bwx116_agy581_1-500th_cs1_lengths.txt
73.33 KB
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Fig_5D_-_bwx092_1-100th_extract.txt
23.06 KB
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Fig_5D_septin_length_based_on_density_recomb_vs_extract.xlsx
22.65 KB
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Fig_5E_-_extract_1-3000_final_80ms_001.nd2
14.57 MB
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Fig_5E_-_extract_1-3000_final_80ms_002.nd2
14.57 MB
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Fig_5E_-_extract_1-3000_final_80ms_003.nd2
14.57 MB
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Fig_5E_-_extract_1-3000_final_80ms_004.nd2
14.57 MB
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Fig_5E_-_extract_1-3000_final_80ms_005.nd2
14.57 MB
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Fig_6A_-_bwx116_AGY581_1-500th_3_hours_2nd_cs_025.nd2
29.01 MB
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Fig_6A_-_bwx116_filament_fluorescence.xlsx
473.35 KB
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Fig_6C_-_bwx118_AGY698_cla4DEL_30_min_127.nd2
144.52 MB
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Fig_6C_-_bwx127_AGY698_aged_1-40_30_min_016.nd2
29.01 MB
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Fig_6C_&_E_-_bwx097_AGY696_gic1D_gic2D_1-10_dilution_019.nd2
144.52 MB
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Fig_6D_-_AGY581_aged_1-800_2_hr_min_040.nd2
29.01 MB
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Fig_6D_-_AGY581_aged_1-800_2_hr_min_041.nd2
29.01 MB
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Fig_6D_-_AGY581_aged_1-800_2_hr_min_042.nd2
29.01 MB
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Fig_6D_-_AGY696_aged_1-800_2_hr_min_037.nd2
29.01 MB
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Fig_6D_-_AGY696_aged_1-800_2_hr_min_038.nd2
29.01 MB
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Fig_6D_-_AGY696_aged_1-800_2_hr_min_039.nd2
29.01 MB
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Fig_6D_-_bwx127_filament_lengths_vs_density.xlsx
250.88 KB
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Fig_6E_-_AGY581_tenth_dilution_037.tif
1.50 MB
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Fig_6E_-_AGY699_cla4D_1-10_dilution_2nd_cs_033.nd2
144.52 MB
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Fig_6F_-_AGY581_wt_1-10_dilution_049.nd2
144.52 MB
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Fig_6F_-_AGY696_gic1D_gic2D_1-10_dilution_010.nd2
144.52 MB
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Fig_6F_-_AGY699_cla4D_1-10_dilution_2nd_cs_025.nd2
144.52 MB
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Fig_6F_-_bwx097_relative_filament_fluorescence.xlsx
507.83 KB
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Fig_6G_-_bwx053_AGY634_Cdc3-mC_Cla4-GFP_10_sec_005.nd2
352.31 MB
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Fig_8A_-_bwx068_A2-_wt_extract_20_min_24324144125144_scaled_&_stitched_blue_thin_skeleton.tif
3.23 MB
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Fig_8A_-_bwx096_AGY581_dilute_combine_trace_thresholded_resized.tif
1.33 MB
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Fig3A&D_Table_1_-_bwx117_20_pM_cs1_008.nd2
577.68 MB
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Fig3A&D_Table_1_-_bwx117_20_pM_cs1_009.nd2
577.68 MB
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Fig3A&D_Table_1_-_bwx117_20_pM_cs1_010.nd2
577.68 MB
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Fig3A&D_Table_1_-_bwx117_20_pM_cs1_011.nd2
577.68 MB
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Fig3A&D_Table_1_-_bwx117_20_pM_cs1_012.nd2
577.68 MB
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Fig3A&D_Table_1_-_bwx117_20_pM_cs1_013.nd2
577.68 MB
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Fig3A&D._Table_1_-_bwx115_11alpha6_20_pM_007.nd2
577.68 MB
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Fig5D_-_bwx116_agy581_1-500th_cs2_lengths.txt
45.58 KB
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Image_source_from_BWX114.txt
40 B
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README_master.txt
3.75 KB
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septins_1000aFrac0.1_energy16.0_flex311.0_pghost1.0.gsd
658.73 MB
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septins_1000aFrac0.25_energy16.0_flex311.0_pghost1.0.gsd
658.81 MB
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septins_1000aFrac0.5_energy16.0_flex311.0_pghost1.0.gsd
658.79 MB
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septins_1000aFrac0.75_energy16.0_flex311.0_pghost1.0.gsd
675.87 MB
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septins_1000aFrac0.9_energy16.0_flex311.0_pghost1.0.gsd
677.58 MB
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septins_1000aFrac1.0_energy16.0_flex311.0_pghost1.0.gsd
658.80 MB
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septins_2000aFrac1.0_energy16.0_flex311.0_pghost1.0.gsd
1.32 GB
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septins_3000aFrac1.0_energy16.0_flex311.0_pghost1.0.gsd
1.96 GB
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septins_4000aFrac1.0_energy16.0_flex311.0_pghost1.0.gsd
2.60 GB
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septins_5000aFrac0.1_energy16.0_flex311.0_pghost0.1.gsd
4.67 GB
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septins_5000aFrac0.1_energy16.0_flex311.0_pghost0.5.gsd
2.68 GB
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septins_5000aFrac0.25_energy16.0_flex311.0_pghost0.01.gsd
6.85 GB
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septins_5000aFrac0.25_energy16.0_flex311.0_pghost0.25.gsd
5.49 GB
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septins_5000aFrac0.25_energy16.0_flex311.0_pghost0.75.gsd
3.51 GB
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septins_5000aFrac0.25_energy18.0_flex28.0_pghost0.1.gsd
4.86 GB
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septins_5000aFrac0.25_energy18.0_flex3.11_pghost0.1.gsd
5.97 GB
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septins_5000aFrac0.25_energy18.0_flex3.11_pghost0.5.gsd
3.47 GB
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septins5000_aFrac0.25_koff0.00015_pghost0.1_friction1.0_energy16.0_flex311.0.gsd
5.60 GB
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septins5000_aFrac0.25_koff0.00015_pghost0.5_friction1.0_energy16.0_flex311.0.gsd
3.89 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy12.0_flex311.0.gsd
5.08 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy14.0_flex311.0.gsd
4.31 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy16.0_flex28.0.gsd
6.23 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy16.0_flex2800.0.gsd
4.78 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy16.0_flex3.11.gsd
4.51 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy16.0_flex311.0.gsd
6.31 GB
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septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy16.0_flex31100.0.gsd
4.75 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy18.0_flex311.0.gsd
6.15 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.1_friction1.0_energy20.0_flex311.0.gsd
5.51 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.1_friction10.0_energy16.0_flex311.0.gsd
4.91 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.1_friction3.3_energy16.0_flex311.0.gsd
4.48 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy12.0_flex311.0.gsd
3.70 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy14.0_flex311.0.gsd
2.99 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy16.0_flex28.0.gsd
3.81 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy16.0_flex2800.0.gsd
3.28 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy16.0_flex3.11.gsd
2.97 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy16.0_flex311.0.gsd
4.41 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy16.0_flex31100.0.gsd
3.24 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy18.0_flex311.0.gsd
4.20 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction1.0_energy20.0_flex311.0.gsd
4.36 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction10.0_energy16.0_flex311.0.gsd
4.38 GB
-
septins5000_aFrac0.25_koff0.00045_pghost0.5_friction3.3_energy16.0_flex311.0.gsd
3.09 GB
-
septins5000_aFrac0.25_koff0.0015_pghost0.1_friction1.0_energy16.0_flex311.0.gsd
4.27 GB
-
septins5000_aFrac0.25_koff0.0015_pghost0.5_friction1.0_energy16.0_flex311.0.gsd
3.23 GB
-
septins5000_aFrac0.25_koff0.0045_pghost0.1_friction1.0_energy16.0_flex311.0.gsd
2.81 GB
-
septins5000_aFrac0.25_koff0.0045_pghost0.5_friction1.0_energy16.0_flex311.0.gsd
3.07 GB
-
septins5000_aFrac0.25_koff4.5e-05_pghost0.1_friction1.0_energy16.0_flex311.0.gsd
6.10 GB
-
septins5000_aFrac0.25_koff4.5e-05_pghost0.5_friction1.0_energy16.0_flex311.0.gsd
5.30 GB
-
Table_1_-_FCS_diffusion_in_solution.xlsx
10.82 KB
-
wt_1_nM_1st_cs_039.nd2
14.57 MB
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wt_1_nM_1st_cs_040.nd2
14.57 MB
-
wt_1_nM_1st_cs_041.nd2
14.57 MB
-
wt_1_nM_1st_cs_042.nd2
14.57 MB
-
wt_1_nM_1st_cs_043.nd2
14.57 MB
-
wt_1_nM_1st_cs_044.nd2
14.57 MB
-
wt_1_nM_1st_cs_045.nd2
14.57 MB
-
wt_1_nM_1st_cs_046.nd2
14.57 MB
-
wt_1_nM_1st_cs_047.nd2
14.57 MB
-
wt_1_nM_1st_cs_048.nd2
14.57 MB
-
wt_1_nM_1st_cs_049.nd2
14.57 MB
-
wt_1_nM_1st_cs_050.nd2
14.57 MB
-
wt_1_nM_2nd_cs_051.nd2
14.57 MB
-
wt_1_nM_2nd_cs_052.nd2
14.57 MB
-
wt_1_nM_2nd_cs_053.nd2
14.57 MB
-
wt_1_nM_2nd_cs_054.nd2
14.57 MB
-
wt_1_nM_2nd_cs_055.nd2
14.57 MB
-
wt_1_nM_2nd_cs_056.nd2
14.57 MB
-
wt_1_nM_2nd_cs_057.nd2
14.57 MB
-
wt_1_nM_2nd_cs_058.nd2
14.57 MB
-
wt_1_nM_2nd_cs_059.nd2
14.57 MB
-
wt_1_nM_2nd_cs_060.nd2
14.57 MB
-
wt_1_nM_2nd_cs_061.nd2
14.57 MB
-
wt_1_nM_35_hours_007.nd2
29.01 MB
-
wt_1_nM_35_hours_008.nd2
29.01 MB
-
wt_1_nM_35_hours_009.nd2
29.01 MB
-
wt_1_nM_35_hours_010.nd2
29.01 MB
-
wt_1_nM_35_hours_011.nd2
29.01 MB
-
wt_1_nM_35_hours_012.nd2
29.01 MB
-
wt_1_nM_35_hours_013.nd2
29.01 MB
-
wt_1_nM_35_hours_014.nd2
29.01 MB
-
wt_1_nM_35_hours_015.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_016.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_017.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_018.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_019.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_020.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_021.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_022.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_023.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_024.nd2
29.01 MB
-
wt_1_nM_35_hours_2nd_cs_025.nd2
29.01 MB
-
wt_10_nM_1st_cs_046.nd2
29.01 MB
-
wt_10_nM_1st_cs_047.nd2
29.01 MB
-
wt_10_nM_1st_cs_048.nd2
29.01 MB
-
wt_10_nM_1st_cs_049.nd2
29.01 MB
-
wt_10_nM_1st_cs_050.nd2
29.01 MB
-
wt_10_nM_1st_cs_051.nd2
29.01 MB
-
wt_10_nM_1st_cs_052.nd2
29.01 MB
-
wt_10_nM_1st_cs_053.nd2
29.01 MB
-
wt_10_nM_1st_cs_054.nd2
29.01 MB
-
wt_10_nM_1st_cs_055.nd2
29.01 MB
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wt_10_nM_1st_cs_079.nd2
14.57 MB
-
wt_10_nM_1st_cs_080.nd2
14.57 MB
-
wt_10_nM_1st_cs_081.nd2
14.57 MB
-
wt_10_nM_1st_cs_082.nd2
14.57 MB
-
wt_10_nM_1st_cs_083.nd2
14.57 MB
-
wt_10_nM_1st_cs_084.nd2
14.57 MB
-
wt_10_nM_1st_cs_085.nd2
14.57 MB
-
wt_10_nM_1st_cs_086.nd2
14.57 MB
-
wt_10_nM_1st_cs_087.nd2
14.57 MB
-
wt_10_nM_1st_cs_088.nd2
14.57 MB
-
wt_10_nM_2nd_cs_056.nd2
29.01 MB
-
wt_10_nM_2nd_cs_057.nd2
29.01 MB
-
wt_10_nM_2nd_cs_058.nd2
29.01 MB
-
wt_10_nM_2nd_cs_059.nd2
29.01 MB
-
wt_10_nM_2nd_cs_060.nd2
29.01 MB
-
wt_10_nM_2nd_cs_061.nd2
29.01 MB
-
wt_10_nM_2nd_cs_062.nd2
29.01 MB
-
wt_10_nM_2nd_cs_063.nd2
29.01 MB
-
wt_10_nM_2nd_cs_064.nd2
29.01 MB
-
wt_10_nM_2nd_cs_089.nd2
14.57 MB
-
wt_10_nM_2nd_cs_090.nd2
14.57 MB
-
wt_10_nM_2nd_cs_091.nd2
14.57 MB
-
wt_10_nM_2nd_cs_092.nd2
14.57 MB
-
wt_10_nM_2nd_cs_093.nd2
14.57 MB
-
wt_10_nM_2nd_cs_094.nd2
14.57 MB
-
wt_10_nM_2nd_cs_095.nd2
14.57 MB
-
wt_10_nM_2nd_cs_096.nd2
14.57 MB
-
wt_10_nM_2nd_cs_097.nd2
14.57 MB
-
wt_100_pM_004.nd2
14.57 MB
-
wt_100_pM_005.nd2
14.57 MB
-
wt_100_pM_006.nd2
14.57 MB
-
wt_100_pM_007.nd2
14.57 MB
-
wt_100_pM_008.nd2
14.57 MB
-
wt_100_pM_009.nd2
14.57 MB
-
wt_100_pM_010.nd2
14.57 MB
-
wt_100_pM_011.nd2
14.57 MB
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wt_100_pM_1st_cs_030.nd2
14.57 MB
-
wt_100_pM_1st_cs_031.nd2
14.57 MB
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wt_100_pM_1st_cs_032.nd2
14.57 MB
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wt_100_pM_1st_cs_033.nd2
14.57 MB
-
wt_100_pM_1st_cs_034.nd2
14.57 MB
-
wt_100_pM_1st_cs_035.nd2
14.57 MB
-
wt_100_pM_1st_cs_036.nd2
14.57 MB
-
wt_100_pM_1st_cs_037.nd2
14.57 MB
-
wt_100_pM_1st_cs_038.nd2
14.57 MB
-
wt_100_pM_1st_cs_039.nd2
14.57 MB
-
wt_100_pM_1st_cs_040.nd2
14.57 MB
-
wt_100_pM_2nd_cs_012.nd2
14.57 MB
-
wt_100_pM_2nd_cs_013.nd2
14.57 MB
-
wt_100_pM_2nd_cs_014.nd2
14.57 MB
-
wt_100_pM_2nd_cs_015.nd2
14.57 MB
-
wt_100_pM_2nd_cs_016.nd2
14.57 MB
-
wt_100_pM_2nd_cs_017.nd2
14.57 MB
-
wt_100_pM_2nd_cs_018.nd2
14.57 MB
-
wt_100_pM_2nd_cs_019.nd2
14.57 MB
-
wt_100_pM_2nd_cs_020.nd2
14.57 MB
-
wt_100_pM_2nd_cs_041.nd2
14.57 MB
-
wt_100_pM_2nd_cs_042.nd2
14.57 MB
-
wt_100_pM_2nd_cs_043.nd2
14.57 MB
-
wt_100_pM_2nd_cs_044.nd2
14.57 MB
-
wt_100_pM_2nd_cs_045.nd2
14.57 MB
-
wt_100_pM_2nd_cs_046.nd2
14.57 MB
-
wt_100_pM_2nd_cs_047.nd2
14.57 MB
-
wt_100_pM_2nd_cs_048.nd2
14.57 MB
-
wt_100_pM_2nd_cs_049.nd2
14.57 MB
-
wt_100_pM_2nd_cs_050.nd2
14.57 MB
-
wt_100_pM_2nd_cs_051.nd2
14.57 MB
-
wt_100_pM_2nd_cs_052.nd2
14.57 MB
-
wt_100_pM_2nd_cs_053.nd2
14.57 MB
-
wt_2_nM_1st_cs_054.nd2
14.57 MB
-
wt_2_nM_1st_cs_055.nd2
14.57 MB
-
wt_2_nM_1st_cs_056.nd2
14.57 MB
-
wt_2_nM_1st_cs_057.nd2
14.57 MB
-
wt_2_nM_1st_cs_058.nd2
14.57 MB
-
wt_2_nM_1st_cs_059.nd2
14.57 MB
-
wt_2_nM_1st_cs_060.nd2
14.57 MB
-
wt_2_nM_1st_cs_062.nd2
14.57 MB
-
wt_2_nM_1st_cs_063.nd2
14.57 MB
-
wt_2_nM_1st_cs_064.nd2
14.57 MB
-
wt_2_nM_1st_cs_065.nd2
14.57 MB
-
wt_2_nM_1st_cs_066.nd2
14.57 MB
-
wt_2_nM_1st_cs_067.nd2
14.57 MB
-
wt_2_nM_1st_cs_068.nd2
14.57 MB
-
wt_2_nM_1st_cs_069.nd2
14.57 MB
-
wt_2_nM_1st_cs_070.nd2
14.57 MB
-
wt_2_nM_1st_cs_071.nd2
14.57 MB
-
wt_2_nM_4_hr_140.nd2
14.57 MB
-
wt_2_nM_4_hr_141.nd2
14.57 MB
-
wt_2_nM_4_hr_142.nd2
14.57 MB
-
wt_2_nM_4_hr_143.nd2
14.57 MB
-
wt_200_pM_35_hours_1st_cs_026.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_027.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_028.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_029.nd2
29.01 MB
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wt_200_pM_35_hours_1st_cs_031.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_032.nd2
29.01 MB
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wt_200_pM_35_hours_1st_cs_033.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_034.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_035.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_036.nd2
29.01 MB
-
wt_200_pM_35_hours_1st_cs_037.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_040.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_041.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_042.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_043.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_044.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_045.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_046.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_047.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_048.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_049.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_050.nd2
29.01 MB
-
wt_200_pM_35_hours_3rd_cs_051.nd2
29.01 MB
-
wt_4_nM_029.nd2
29.01 MB
-
wt_4_nM_030.nd2
29.01 MB
-
wt_4_nM_031.nd2
29.01 MB
-
wt_4_nM_032.nd2
29.01 MB
-
wt_4_nM_033.nd2
29.01 MB
-
wt_4_nM_034.nd2
29.01 MB
-
wt_4_nM_035.nd2
29.01 MB
-
wt_4_nM_036.nd2
29.01 MB
-
wt_4_nM_2nd_cs_037.nd2
29.01 MB
-
wt_4_nM_2nd_cs_038.nd2
29.01 MB
-
wt_4_nM_2nd_cs_039.nd2
29.01 MB
-
wt_4_nM_2nd_cs_040.nd2
29.01 MB
-
wt_4_nM_2nd_cs_041.nd2
29.01 MB
-
wt_4_nM_2nd_cs_042.nd2
29.01 MB
-
wt_4_nM_2nd_cs_043.nd2
29.01 MB
-
wt_4_nM_2nd_cs_044.nd2
29.01 MB
-
wt_4_nM_2nd_cs_045.nd2
29.01 MB
-
wt_400_pM_4_hr_132.nd2
14.57 MB
-
wt_400_pM_4_hr_133.nd2
14.57 MB
-
wt_400_pM_4_hr_134.nd2
14.57 MB
-
wt_400_pM_4_hr_135.nd2
14.57 MB
-
wt_600_pM_4_hr_136.nd2
14.57 MB
-
wt_600_pM_4_hr_137.nd2
14.57 MB
-
wt_600_pM_4_hr_138.nd2
14.57 MB
-
wt_600_pM_4_hr_139.nd2
14.57 MB
-
wt-SNAP488_300_pM_002.nd2
29.01 MB
-
wt-SNAP488_300_pM_003.nd2
29.01 MB
-
wt-SNAP488_300_pM_004.nd2
29.01 MB
-
wt-SNAP488_300_pM_005.nd2
29.01 MB
-
wt-SNAP488_300_pM_montage_skeleton_ave_Cs.txt
6.12 KB
-
wt-SNAP488_300_pM_montage_skeleton_bending_modes.txt
147 KB
-
wt-SNAP488_300_pM_montage_skeleton_contours.txt
2.16 MB
-
wt-SNAP488_300_pM_montage_skeleton_Cs_raw.txt
58.40 MB
-
wt-SNAP488_300_pM_montage_skeleton_end_to_end_vs_contour_lengths.txt
17.14 KB
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wt-SNAP488_300_pM_montage_skeleton_select.mat
594.48 KB
-
wt-SNAP488_300_pM_montage_skeleton_tangent_angles.txt
2.16 MB
-
wt-SNAP488_300_pM_montage_skeleton.tif
4.32 MB
-
wt-SNAP488_300_pM_montage_threshold.tif
4.32 MB
-
wt-SNAP488_4_nM_1st_cs_051.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_052.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_053.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_054.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_055.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_057.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_058.nd2
14.57 MB
-
wt-SNAP488_4_nM_1st_cs_059.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_060.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_061.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_062.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_063.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_064.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_065.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_066.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_067.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_068.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_069.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_070.nd2
14.57 MB
-
wt-SNAP488_4_nM_2nd_cs_071.nd2
14.57 MB
-
wt-SNAP488_400_pM_018.nd2
14.57 MB
-
wt-SNAP488_400_pM_019.nd2
14.57 MB
-
wt-SNAP488_400_pM_020.nd2
14.57 MB
-
wt-SNAP488_400_pM_021.nd2
14.57 MB
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wt-SNAP488_400_pM_1st_cs_001.nd2
29.01 MB
-
wt-SNAP488_400_pM_1st_cs_002.nd2
29.01 MB
-
wt-SNAP488_400_pM_1st_cs_003.nd2
29.01 MB
-
wt-SNAP488_400_pM_1st_cs_004.nd2
29.01 MB
-
wt-SNAP488_400_pM_1st_cs_005.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_006.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_007.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_008.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_009.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_010.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_011.nd2
14.57 MB
-
wt-SNAP488_400_pM_1st_cs_012.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_014.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_015.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_016.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_017.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_018.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_019.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_020.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_021.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_022.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_023.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_024.nd2
14.57 MB
-
wt-SNAP488_400_pM_2nd_cs_025.nd2
14.57 MB
-
wt-SNAP488_400_pM_time_lapse_001-5.nd2
524.71 MB
-
wt-SNAP488_600_pM_1st_cs_026.nd2
14.57 MB
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wt-SNAP488_600_pM_1st_cs_027.nd2
14.57 MB
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wt-SNAP488_600_pM_1st_cs_028.nd2
14.57 MB
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wt-SNAP488_600_pM_1st_cs_029.nd2
14.57 MB
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wt-SNAP488_600_pM_1st_cs_030.nd2
14.57 MB
Abstract
Septin filaments build structures such as rings, lattices and gauzes that serve as platforms for localizing signaling and organizing cell membranes. How cells control the geometry of septin assemblies in poorly understood. We show here that septins are isodesmic polymers, in contrast to cooperative polymerization exhibited by F-actin and microtubules. We constructed a physical model to analyze and interpret how septin assemblies change in the presence of regulators in yeast extracts. Notably filaments differ in length and curvature in yeast extract compared to pure protein indicating cellular regulators modulate intrinsic biophysical features. Combining analysis of extracts from regulatory mutants with simulations, we found increased filament flexibility and reduced filament fragmentation promote assembly of septin rings, whereas reduced flexibility in crowded environments promotes local filament alignment. This work demonstrates how tuning of intrinsic features of septin filament assembly by regulatory proteins yields a diverse array of structures observed in cells.
Images were acquired via TIRF microscopy after incubating septins/extracts on planar supported lipid bilayers. Images were prepared using FIJI/ImageJ.