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Experimental and synthetic datasets supporting FITSA: Statistical analysis of fluorescence intensity transients with Bayesian methods

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Mar 17, 2025 version files 12.31 GB

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Abstract

This dataset supports our study "Statistical Analysis of Fluorescence Intensity Transients with Bayesian Methods," which introduces Fluorescence Intensity Trace Statistical Analysis (FITSA), a Bayesian approach for direct analysis of fluorescence intensity traces. From these traces, FITSA estimates diffusion coefficient and molecular brightness. The repository contains all fluorescence intensity traces used in our comparative analysis of FITSA and fluorescence correlation spectroscopy (FCS). A README file describes the data structure. We provide both synthetic and experimental datasets that demonstrate various applications of FITSA. When combined with our separately published code, these datasets enable reproduction of our analysis and support further methodological development in the field. Based on our analysis of these traces, we demonstrate that FITSA achieves precision comparable to FCS while requiring substantially fewer photons and shorter measurement times.