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Contrasting phylogeographic patterns of sandy vs. rocky sympatric sister species of supralittoral Tylos isopods in Chile

Hurtado, Luis1; Mateos, Mariana1; Wang, Chang1; Ndeda, Violet1; Pérez-Schultheiss, Jorge2; Thiel, Martin345

Published Jul 16, 2025 on Dryad. https://doi.org/10.5061/dryad.8kprr4xzr

Data files

Jul 16, 2025 version files 2.33 MB

Abstract

Sister taxa that have diverged and persisted in sympatry have likely been exposed to the same general environmental changes throughout their evolutionary history and may thus exhibit similar phylogeographies. Here we compare the phylogeographic patterns of two sister species of isopods (genus Tylos) that have broadly overlapping distributions but distinct habitat preferences in the supralittoral zone of Chile. The dynamic geoclimatic history of this region during the Quaternary has been implicated in shaping the evolutionary histories of other coastal taxa. Tylos spinulosus is found in sandy beaches at latitudes ~27–30°S, whereas Tylos chilensis has been found in rocky shores at ~27–33°S and at ~39–42°S. We sampled both species across their ranges (collectively from 20 localities) and obtained sequences from at least one mitochondrial gene for 95 T. chilensis and 41 T. spinulosus. We used phylogenetics and population genetics methods to analyze four single-gene and one concatenated datasets: 12S rDNA (n=130); 16S rDNA (n=31); Cytochrome oxidase subunit I (n=28); Cytochrome b (n=24); concatenation of the four genes (n=24). Both species show high levels of isolation of local populations, consistent with expectations from their limited autonomous dispersal potential. However, they exhibit strikingly different mitochondrial phylogeographic patterns. Tylos chilensis shows evidence of multiple relatively deep divergence events leading to geographically restricted lineages that appear to have persisted over multiple glaciations. Surprisingly, one lineage of T. chilensis was found in geographically distant localities, suggesting the possibility of human-mediated dispersal. Tylos spinulosus appears to have undergone a relatively recent bottleneck followed by a population/range expansion. Differences in life histories and habitat preferences or stochasticity may have contributed to these striking phylogeographic differences. Finally, the high levels of differentiation and isolation among populations indicate that they are highly vulnerable to extirpation. We discuss threats to their persistence, and recommendations for their conservation.

This dataset includes: details about each of the specimens used in this study (Supporting Table S1); input and output files used to generate the haplotype networks depicted in the manuscripts; input and output files of the analyses used to generate the rooted phylogenies depicted in this manuscript (5 datasets: four single-gene and one concatenated); command line and input files used to infer isolation by distance in Tylos spinulosus (Mante test); and input and output files and detailed methodology for testing the molecular clock hypothesis and inferring genetic distances for two genes.