The current understanding of proximodistal axis patterning in arthropod legs is grounded in insect models. The paradigm for appendage evolution in this phylum is that the gene regulatory network responsible for leg subdivision and patterning is broadly conserved. Recent surveys of these genes have suggested that chelicerate exemplars exhibit divergent appendage patterning dynamics, though functional data remain limited. One salient mismatch in expression occurs in homologs of the homeobox gene clawless. In insects, clawless is expressed in the distalmost leg territory, specifying the claw-bearing pretarsus. In the harvestman Phalangium opilio, clawless occupies a broad tarsal domain early in development, localizing later to the metatarsus-tarsus boundary, suggestive of a tarsal patterning function. Here, we tested the function of harvestman clawless using RNAi. Unlike insects, we show that clawless knockdown results in disrupted tarsal growth and patterning of its proximal segmental boundary, with no effect on the claw. Truncation of the tarsus is associated with defective tarsomere formation. We additionally surveyed the expression of clawless homologs in exemplars of chelicerate diversity, which suggests that the tarsal-patterning function for clawless was likely present in the most recent common ancestor of Chelicerata. These results join a suite of other comparative works suggesting that panarthropod appendage patterning exhibits numerous cases of developmental system drift.

Dataset DOI: 10.5061/dryad.95x69p8zs

Description of the data and file structure

The collected data consist of hyperstacks of confocal laser scanning microscopy images, in raw format. Each stack contains some or all of the following channels: 405 nm (blue); 488 nm (green); 546 nm (red); 594 nm (red); 647 nm (far red). The numbering of the files corresponds the numbering of the figures in the associated paper.

Files and variables

File: FigureS6e.Plit_InsVI_03_le1WL_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S6e. Distal podomeres of leg 1 in the sea spider Pycnogonum litorale (instar VI) with expression of clawless and aristaless. Following complete separation of tarsus and propodus, only a small region of co-expression remains in the dorsal tarsus joint. The claw retains non-overlapping expression of aristaless in the dorsal compartment, and clawless in the ventral. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: Figure4c.S6d.Plit_InsV_16_ri1WL_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figures 4c' and S6d. Leg 1 in the sea spider Pycnogonum litorale (instar V) with expression of clawless and aristaless. Following complete separation of tarsus and propodus, only a small region of co-expression remains in the dorsal tarsus joint. The claw retains non-overlapping expression of aristaless in the dorsal compartment, and clawless in the ventral. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: Figure4c.S6b.Plit_InsIV_65_HB_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figures 4c and S6b. Ventral view of posterior body pole and leg 2 limb bud in the sea spider Pycnogonum litorale (instar IV) with expression of clawless and aristaless. Following outgrowth of the limb buds from the body wall, aristaless is expressed in a wide domain in the proximal limb bud and the distal terminus, whereas clawless occupies the intervening region with a sharp proximal border at the boundary of aristaless expression. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: FigureS6c.Plit_InsIV_54_ri1WL_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S6c. Leg 1 in the sea spider Pycnogonum litorale (instar IV) with expression of clawless and aristaless. At this stage, clawless is expressed in the tarsus-propodus precursor podomere and ventral compartment of the claw. aristaless is expressed diffusely in all further proximal podomeres, and strongly in the dorsal compartment of the claw. A narrow ring of co-expression occurs at the proximal boundary of clawless expression. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: FigureS5d.Plit_InsIV_54_OV_20x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S5d. Ventral view of the sea spider Pycnogonum litorale (instar IV) with expression of clawless and aristaless. Following outgrowth of the limb buds from the body wall, aristaless is expressed in a wide domain in the proximal limb bud and the distal terminus, whereas clawless occupies the intervening region with a sharp proximal border at the boundary of aristaless expression. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: FigureS6a.Plit_InsIII_82_lat_OV_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S6a. Lateral view of the sea spider Pycnogonum litorale (instar III) with expression of clawless and aristaless. Following outgrowth of the limb buds from the body wall, aristaless is expressed in a wide domain in the proximal limb bud and the distal terminus, whereas clawless occupies the intervening region with a sharp proximal border at the boundary of aristaless expression. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: FigureS5b.Plit_InsIII_69_OV_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S5b. Ventral view of the sea spider Pycnogonum litorale (instar III, early) with expression of clawless and aristaless. Following outgrowth of the limb buds from the body wall, aristaless is expressed in a wide domain in the proximal limb bud and the distal terminus, whereas clawless occupies the intervening region with a sharp proximal border at the boundary of aristaless expression. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: FigureS5c.Plit_InsIII_68_OV_40x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S5c. Ventral view of the sea spider Pycnogonum litorale (instar III, late) with expression of clawless and aristaless. Following outgrowth of the limb buds from the body wall, aristaless is expressed in a wide domain in the proximal limb bud and the distal terminus, whereas clawless occupies the intervening region with a sharp proximal border at the boundary of aristaless expression. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: FigureS5a.Plit_InsII_34_OV_63x_Dapi.AF.al-647_Cll-594_TDE.lif

Description: Confocal microscopy hyperstack corresponding to Figure S5a. Ventral view of the sea spider Pycnogonum litorale (instar II, late) with expression of clawless and aristaless. At this stage, both clawless and aristaless are detected in the leg 1 primordium flanking the posterior body pole. Both genes are partially co-expressed at the distal-most area of the primordium; the more restricted Pl-al territory is embedded in the wider cap-like Pl-cll expression domain. In combination with expression surveys spanning postembryonic development, these data support conserved dynamics of both genes across Chelicerata, and the homology of arachnid metatarsus, tarsus, and pretarsus, with the sea spider tarsus, propodus, and claw. Channels correspond to DAPI (405nm), cuticular autofluorescence (488nm), clawless (594nm), and aristaless (647nm).

File: Figure1b.WT.wholemount.al488.cll546.czi

Description: Confocal microscopy hyperstack corresponding to Figure 1b. Embryo of the harvestman Phalangium opilio (stage 10) in ventral view with expression of clawless and aristaless. At this stage, clawless is expressed broadly throughout the distal appendage, whereas aristaless is detected in a ring domain proximal of the clawless territory, and weakly in the distal terminus. This broad clawless expression represents divergence from mandibulate expression patterns, providing initial evidence of developmental system drift in this lineage. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure1d.WT.wholemount.al488.cll546.czi

Description: Confocal microscopy hyperstack corresponding to Figure 1d. Embryo of the harvestman Phalangium opilio (stage 13) in ventral view with expression of clawless and aristaless. At this stage, clawless expression localizes to a prominent ring domain at the metatarsus-tarsus podomere boundary. This expression pattern is suggestive of a role in podomere formation, rather than tarsal claw formation as in mandibulate arthropods. aristaless is detected in diffuse patches of expression in more proximal podomeres and the distal terminus. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure1c.WT.L2.al488.cll546.czi

Description: Confocal microscopy hyperstack corresponding to Figure 1c. Leg 2 of the harvestman Phalangium opilio (stage 10) with expression of clawless and aristaless. At this stage, clawless is expressed broadly throughout the distal appendage, whereas aristaless is detected in a ring domain proximal of the clawless territory, and weakly in the distal terminus. This broad clawless expression represents divergence from mandibulate expression patterns, providing initial evidence of developmental system drift in this lineage. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure1e.WT.L2.al488.cll546.czi

Description: Confocal microscopy hyperstack corresponding to Figure 1e (Phalangium opilio). Leg II of the harvestman Phalangium opilio (stage 13) with expression of clawless and aristaless. At this stage, clawless expression localizes to a prominent ring domain at the metatarsus-tarsus podomere boundary. This expression pattern is suggestive of a role in podomere formation, rather than tarsal claw formation as in mandibulate arthropods. aristaless is detected in diffuse patches of expression in more proximal podomeres and the distal terminus. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure2a.WT.wholemount.Dl546.al647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 2a. Negative control embryo of Phalangium opilio (stage 11) with expression of Delta and aristaless. Note that at this stage, Delta is expressed in multiple ring domains in the tarsus, indicative of sequential tarsomere formation. Channels correspond to DAPI (405nm), Delta (546nm), and aristaless (647nm).

File: Figure2b.cllRNAi.wholemount.dl546.al647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 2b. Embryo of Phalangium opilio (stage 11) following RNAi against clawless with expression of Delta and aristaless. In contrast to negative control embryos, depletion of clawless yields disruptions to Delta ring domains in the tarsus, consistent with abolished tarsomere formation. Lingering patches of Delta expression correspond to putative sensory structures. Channels correspond to DAPI (405nm), Delta (546nm), and aristaless (647nm).

File: Figure2c.WT.Leg2.dl546.al647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 2c. Leg 2 of negative control Phalangium opilio embryo (stage 11) with expression of Delta and aristaless. Note that at this stage, Delta is expressed in multiple ring domains in the tarsus, indicative of sequential tarsomere formation, with the highest intensity expression in the proximal tarsus. Channels correspond to DAPI (405nm), Delta (546nm), and aristaless (647nm).

File: Figure4a.S4c.Alon.al.cll.czi

Description: Confocal microscopy hyperstack corresponding to Figures 4a and S4c. Embryo of the acariform mite Archegozetes longisetosus in lateral view with expression of clawless and aristaless. At this stage, clawless exhibits a broad cap-like expression domain beyond the aristaless-positive cells in the distal terminus. aristaless is likewise expressed in a small patch in the proximal territory of each appendage. This clawless domain is comparable to the expression domain of clawless in early* P. opilio* appendage development, suggestive of conserved dynamics across Chelicerata. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure2e.WT.L2.odd488.Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure 2e. Leg II of a negative control Phalangium opilio embryo with expression of Delta and odd-skipped. Note the prominent rings of odd-skipped expression at developing podomere boundaries and ring domains of Delta indicative of sequential tarsomere addition. Channels correspond to DAPI (405nm), odd-skipped (488nm), and Delta (546nm).

File: Figure4d.Parhyale.wholemount.cll1546_SoxN647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 4d. Embryo of the amphipod Parhyale hawaiensis (stage 20) with expression of clawless-1. At this stage, clawless-1 is expressed in the distal territory of both antennal pairs and thoracopods 1-8, and in small domains in the central nervous system. Note that expression in the thoracopods exhibits two discrete patches. Channels correspond to DAPI (405nm) and clawless-1 (546nm).

File: Figure4e.Parhyale.wholemount.cll2546_soxN647_S20.czi

Description: Confocal microscopy hyperstack corresponding to Figure 4e. Embryo of the amphipod Parhyale hawaiensis (stage 22) with expression of clawless-2. Both clawless paralogs in this species exhibit comparable dynamics, with clawless-2 expressed distally in both antennal pairs and thoracopods 1-8, and the central nervous system, albeit more homogeneously than clawless-1. Channels correspond to DAPI (405nm) and clawless-2 (546nm).

File: Figure2d.cllRNAi.Leg2.dl546.al647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 2d. Leg 2 of clawless RNAi Phalangium opilio embryo (stage 11) with expresion of Delta and aristaless. Note the prominent swelling of the appendage at the putative metatarsus-tarsus boundary, consistent with the inferred role in podomere formation. Disruptions of Delta rings in the tarsus are also consistent with failed tarsomere formation. Channels correspond to DAPI (405nm), Delta (546nm), and aristaless (647nm).

File: Figure4f.Parhyale.al1488_cll1546_SoxN647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 4f. Detail of thoracopods in an embryo of the amphipod Parhyale hawaiensis (stage 22) with expression of clawless-1. At this stage, clawless-1 is expressed in the distal territory of both antennal pairs and thoracopods 1-8, and in small domains in the central nervous system. Note that expression in the thoracopods exhibits two discrete patches in the dactylus and propodus. Channels correspond to DAPI (405nm) and clawless-1 (546nm).

File: Figure2f.cllRNAi.Leg2.odd488.Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure 2f. Leg 2 of a clawless RNAi Phalangium opilio embryo with expression of Delta and odd-skipped. Note the absence of the distalmost ring of odd-skipped in comparison to negative control embryo, consistent with a fusion of metatarsus and tarsus. Disruptions to tarsal rings of Delta again coincide with abolished tarsomere addition. Channels correspond to DAPI (405nm), odd-skipped (488nm), and Delta (546nm).

File: FigureS4d_Alon_al_cll.czi

Description: Confocal microscopy hyperstack corresponding to Figure S4d. Embryo of the acariform mite Archegozetes longisetosus in lateral view with expression of clawless and aristaless. In later stages of development, clawless retains strong expression in a broad cap-like domain in the distal appendage, whereas aristaless is retained in the distal terminus but is weaker and more diffuse in the proximal appendage. This clawless domain is comparable to the expression domain of clawless in early* P. opilio* appendage development, suggestive of conserved dynamics across Chelicerata. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure4b.Isca.st13_cll_al.lif

Description: Confocal microscopy hyperstack corresponding to Figure 4b. Embryos of the parasitiform tick Ixodes scapularis with expression of clawless. Note prominent localization of clawless expression to the boundary of basi- and telotarsus podomeres, similar to the late localization of clawless to metatarsus-tarsus boundary in P. opilio. This expression is again consistent with conserved clawless dynamics across Chelicerata. Channels correspond to DAPI (405nm) and clawless (546nm).

File: FigureS4a_Alon_al_cll.czi

Description: Confocal microscopy hyperstack corresponding to Figure S4a. Embryo of the acariform mite Archegozetes longisetosus in lateral view with expression of clawless and aristaless. At this stage, clawless is broadly expressed in the appendage primordia. In contrast, aristaless expression was not detected. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: FigureS1a.WT.wholemount.odd488.cll546.dll647.czi

Description: Confocal microscopy hyperstack corresponding to Figure S1a. Negative control Phalangium opilio embryo (stage 10) with expression of clawless, odd-skipped, and Distal-less. Note prominent clawless expression in the distal appendage. Channels correspond to DAPI (405nm), odd-skipped (488nm), clawless (546nm), and Distal-less (647nm).

File: FigureS1b.cllRNAi.wholemount.odd488.cll546.dll647.czi

Description: Confocal microscopy hyperstack corresponding to Figure S1b. clawless RNAi Phalangium opilio embryo with expression of clawless, odd-skipped, and Distal-less. RNAi experiments yield on-target depletion of clawless, whereas expression of odd-skipped and Distal-less remains. Channels correspond to DAPI (405nm), odd-skipped (488nm), clawless (546nm), and Distal-less (647nm).

File: FigureS4b_Alon_al_cll.czi

Description: Confocal microscopy hyperstack corresponding to Figure S4b. Embryo of the acariform mite Archegozetes longisetosus in lateral view with expression of clawless and aristaless. At this stage, clawless exhibits a broad cap-like expression domain beyond the aristaless-positive cells in the distal terminus. aristaless is likewise expressed in a small patch in the proximal territory of each appendage. This clawless domain is comparable to the expression domain of clawless in early* P. opilio* appendage development, suggestive of conserved dynamics across Chelicerata. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: Figure4g.Parhyale_al2488_cll2546_SoxN647.czi

Description: Confocal microscopy hyperstack corresponding to Figure 4g. Detail of thoracopods in an embryo of the amphipod Parhyale hawaiensis (stage 24) with expression of clawless-2. At this stage, clawless-2 is expressed comparably to clawless-1, in the distal territory of both antennal pairs and thoracopods 1-8, and in small domains in the central nervous system. Unlike slightly earlier stages, clawless-2 is expressed in two discrete patches. Channels correspond to DAPI (405nm) and *clawless-2 *(546nm).

File: FigureS4e_Alon_al_cll.czi

Description: Confocal microscopy hyperstack corresponding to Figure S4e. Embryo of the acariform mite Archegozetes longisetosus in lateral view with expression of clawless and aristaless. In later stages of development, clawless retains strong expression in a broad cap-like domain in the distal appendage, whereas aristaless is retained in the distal terminus but is weaker and more diffuse in the proximal appendage. This clawless domain is comparable to the expression domain of clawless in early* P. opilio* appendage development, suggestive of conserved dynamics across Chelicerata. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: FigureS3a_WT_St9_ser488_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3a. Embryo of Phalangium opilio (stage 9) in ventral view with expression of Serrate and Delta. To determine whether progressive rings of Delta expression coincide with tarsomere addition, representative stages of leg elongation were surveyed. At this early stage, Serrate is expressed in ring domains corresponding to putative podomere boundaries, whereas Delta is expressed prominently in the central nervous system, and in a weak ring domain in the leg 2 tarsus. As leg 2 will bear the highest number of tarsomeres in this lineage, this early expression may be indicative of earlier onset of tarsomere addition. Channels correspond to DAPI (405nm), Serrate (488nm), and Delta (546nm).

File: FigureS2.cllRNAi.wholemount.dl546.al647.czi

Description: Confocal microscopy hyperstack corresponding to Figure S2. clawless RNAi Phalangium opilio embryo (stage 11) with expression of clawless and aristaless. A subset of RNAi embryos exhibited a pronounced bend or kink in the distal appendage, attributed to asymmetric projections of tissue at the malformed joint between metatarsus and tarsus. Channels correspond to DAPI (405nm), clawless (546nm), and aristaless (647nm).

File: FigureS3c_WT_St11_Ser488_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3c. Embryo of Phalangium opilio (stage 11) in ventral view with expression of Serrate and Delta. To determine whether progressive rings of Delta expression coincide with tarsomere addition, representative stages of leg elongation were surveyed. At this stage, Serrate is still expressed in ring domains corresponding to putative podomere boundaries, whereas Delta is expressed prominently in the central nervous system, and in several ring domains in all leg tarsi. The increased number of rings relative to stage 10 embryos, suggests Delta demarcates the sequentially added tarsomeres. Note the absence of Delta rings in the pedipalpal tarsi, consistent with their lack of tarsomeres. Channels correspond to DAPI (405nm), Serrate (488nm), and Delta (546nm).

File: FigureS3b_WT_wholemount_St10_Ser488_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3b. Embryo of Phalangium opilio (stage 1o) in ventral view with expression of Serrate and Delta. To determine whether progressive rings of Delta expression coincide with tarsomere addition, representative stages of leg elongation were surveyed. At this stage, Serrate is still expressed in ring domains corresponding to putative podomere boundaries, whereas Delta is expressed prominently in the central nervous system. In the tarsi of legs 1, 3, and 4, Delta exhibits one strong ring domain, while exhibiting several such domains in the leg 2 tarsi. The increased number of rings in leg 2 relative to stage 9 embryos, suggests Delta demarcates the sequentially added tarsomeres. Note the absence of Delta rings in the pedipalpal tarsi, consistent with their lack of tarsomeres. Channels correspond to DAPI (405nm), Serrate (488nm), and Delta (546nm).

File: FigureS3d_WT_St12_Ser488_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3d. Embryo of Phalangium opilio (stage 12) in ventral view with expression of Serrate and Delta. To determine whether progressive rings of Delta expression coincide with tarsomere addition, representative stages of leg elongation were surveyed. At this stage, Serrate is still expressed in ring domains corresponding to putative podomere boundaries, whereas Delta is expressed prominently in the central nervous system, and in numerous ring domains in all leg tarsi. The increased number of rings relative to stage 11 embryos, suggests Delta demarcates the sequentially added tarsomeres. Note the absence of Delta rings in the pedipalpal tarsi, consistent with their lack of tarsomeres. Channels correspond to DAPI (405nm), Serrate (488nm), and Delta (546nm).

File: FigureS3e_WT_L2_stage10_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3e. Detail of distal leg 2 in Phalangium opilio embryo (stage 10) with expression of Delta. Note high intensity of expression in rings distal to the tarsus boundary, the putative location of tarsomere addition. Channels correspond to DAPI (405nm) and Delta (546nm).

File: FigureS3f_WT_L2_stage11_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3f (Phalangium opilio). Detail of distal leg 2 in Phalangium opilio embryo (stage 11) with expression of Delta. The increase in number of Delta rings compared to stage 10 is indicative of demarcating sequentially added tarsomeres.**** Channels correspond to DAPI (405nm) and Delta (546nm).

File: FigureS3g_WT_L2_stage12_Dl546.czi

Description: Confocal microscopy hyperstack corresponding to Figure S3g. Detail of distal leg 2 in Phalangium opilio embryo (stage 12) with expression of Delta. Note that in later stages, Delta rings are obscured by expression localized to putative sensory cells. Channels correspond to DAPI (405nm) and Delta (546nm).

Code/software

All .czi and .lif files are readily viewed in Fiji or a similar image processing software (e.g., Zeiss Zen Lite).

Data from: Developmental system drift in the patterning of the arthropod tarsus

Data files

Abstract

README: Data from: Developmental system drift in the patterning of the arthropod tarsus