Data from: Transplantation of exogenous mitochondria mitigates myocardial dysfunction after cardiac arrest
Data files
Apr 17, 2025 version files 39.75 MB
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Data.xlsx
22.33 KB
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Flow_cytometry.zip
39.72 MB
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README.md
9.12 KB
Abstract
The incidence of post-cardiac arrest myocardial dysfunction (PAMD) is high, and there is currently no effective treatment available. This study aims to investigate the protective effects of exogenous mitochondrial transplantation. Exogenous mitochondrial transplantation can enhance myocardial function and improve the survival rate. Mechanistic studies suggest that mitochondrial transplantation can limit impairment in mitochondrial morphology, augment the activity of mitochondrial complexes II and IV, and raise ATP level. As well, mitochondrial therapy ameliorated oxidative stress imbalance, reduced myocardial injury, and thus improved PAMD after cardiopulmonary resuscitation (CPR).
Our study aims to investigate the protective effects of exogenous mitochondrial transplantation on the myocardium following cardiac arrest. We utilize a rat model of asphyxiation-induced cardiac arrest. Once the model is established, the rats are humanely euthanized for sample collection or survival analysis.
Dataset DOI: 10.5061/dryad.9cnp5hqwm
Description of the data
The following files are available:
Apoptosis: The level of the apoptosis in rat myocardium was measured as described in the Methods section: Flow Cytometry. (file format: fcs; file name: Apoptosis)
mPTP: The level of the mitochondrial permeability transition pore (mPTP) in rat myocardium was measured as described in the Methods section: Mitochondrial Swelling Assay. (file format: fcs; file name: mPTP).
ROS: The level of mitochondrial reactive oxygen species (ROS) in rat myocardium was measured as described in the Methods section: Determination of ROS. (file format: fcs; file name: ROS)
Data: The Excel spreadsheet containing the underlying data numerical values that were used to generate the Table / Figures in this publication. (file format: xlsx; file name: Data)
File structure
Excel file Name: Data
The Excel spreadsheet containing the underlying data numerical values that were used to generate the Table / Figures in this publication.
Folder Name: Flow cytometry
Content (3 sub-folders):
1) Sub-folder 1: Apoptosis
Sub-sub-folder1: Mito
Mito1.fcs: The first repeated detection of myocardial apoptosis in the Mito group.
Mito2.fcs: The second repeated detection of myocardial apoptosis in the Mito group.
Mito3.fcs: The third repeated detection of myocardial apoptosis in the Mito group.
Sub-sub-folder2: NS
NS1.fcs: The first repeated detection of myocardial apoptosis in the NS group.
NS2.fcs: The second repeated detection of myocardial apoptosis in the NS group.
NS3.fcs: The third repeated detection of myocardial apoptosis in the NS group.
Sub-sub-folder3: Sham
Sham1.fcs: The first repeated detection of myocardial apoptosis in the Sham group.
Sham2.fcs: The second repeated detection of myocardial apoptosis in the Sham group.
Sham3.fcs: The third repeated detection of myocardial apoptosis in the Sham group.
Sub-sub-folder4: Vehicle
Vehicle1.fcs: The first repeated detection of myocardial apoptosis in the Vehicle group.
Vehicle2.fcs: The second repeated detection of myocardial apoptosis in the Vehicle group.
Vehicle3.fcs: The third repeated detection of myocardial apoptosis in the Vehicle group.
2) Sub-folder 2: mPTP
Sub-sub-folder1: Mito
Calcein AM+CoCl2 1.fcs: The first repeated detection of Calcein AM+CoCl2 fluorescence in the Mito group.
Calcein AM+CoCl2 2.fcs: The second repeated detection of Calcein AM+CoCl2 fluorescence in the Mito group.
Calcein AM+CoCl2 3.fcs: The third repeated detection of Calcein AM+CoCl2 fluorescence in the Mito group.
Calcein AM+CoCl2+lonomycin1.fcs: The first repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Mito group.
Calcein AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Mito group.
Calcein AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Mito group.
Calcein AM1.fcs: The first repeated detection of Calcein AM fluorescence in the Mito group.
Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence in the Mito group.
Calcein AM3.fcs: The third repeated detection of Calcein AM fluorescence in the Mito group.
Negative1.fcs: The first repeated detection of negative fluorescence in the Mito group.
Negative2.fcs: The second repeated detection of negative fluorescence in the Mito group.
Negative3.fcs: The third repeated detection of negative fluorescence in the Mito group.
Sub-sub-folder2: NS
Calcein AM+CoCl2 1.fcs: The first repeated detection of Calcein AM+CoCl2 fluorescence in the NS group.
Calcein AM+CoCl2 2.fcs: The second repeated detection of Calcein AM+CoCl2 fluorescence in the NS group.
Calcein AM+CoCl2 3.fcs: The third repeated detection of Calcein AM+CoCl2 fluorescence in the NS group.
Calcein AM+CoCl2+lonomycin1.fcs: The first repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the NS group.
Calcein AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the NS group.
Calcein AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the NS group.
Calcein AM1.fcs: The first repeated detection of Calcein AM fluorescence in the NS group.
Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence in the NS group.
Calcein AM3.fcs: The third repeated detection of Calcein AM fluorescence in the NS group.
Negative1.fcs: The first repeated detection of negative fluorescence in the NS group.
Negative2.fcs: The second repeated detection of negative fluorescence in the NS group.
Negative3.fcs: The third repeated detection of negative fluorescence in the NS group.
Sub-sub-folder3: Sham
Calcein AM+CoCl2 1.fcs: The first repeated detection of Calcein AM+CoCl2 fluorescence in the Sham group.
Calcein AM+CoCl2 2.fcs: The second repeated detection of Calcein AM+CoCl2 fluorescence in the Sham group.
Calcein AM+CoCl2 3.fcs: The third repeated detection of Calcein AM+CoCl2 fluorescence in the Sham group.
Calcein AM+CoCl2+lonomycin1.fcs: The first repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Sham group.
Calcein AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Sham group.
Calcein AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Sham group.
Calcein AM1.fcs: The first repeated detection of Calcein AM fluorescence in the Sham group.
Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence in the Sham group.
Calcein AM3.fcs: The third repeated detection of Calcein AM fluorescence in the Sham group.
Negative1.fcs: The first repeated detection of negative fluorescence in the Sham group.
Negative2.fcs: The second repeated detection of negative fluorescence in the Sham group.
Negative3.fcs: The third repeated detection of negative fluorescence in the Sham group.
Sub-sub-folder4: Vehicle
Calcein AM+CoCl2 1.fcs: The first repeated detection of Calcein AM+CoCl2 fluorescence in the Vehicle group.
Calcein AM+CoCl2 2.fcs: The second repeated detection of Calcein AM+CoCl2 fluorescence in the Vehicle group.
Calcein AM+CoCl2 3.fcs: The third repeated detection of Calcein AM+CoCl2 fluorescence in the Vehicle group.
Calcein AM+CoCl2+lonomycin1.fcs: The first repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Vehicle group.
Calcein AM+CoCl2+lonomycin2.fcs: The second repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Vehicle group.
Calcein AM+CoCl2+lonomycin3.fcs: The third repeated detection of Calcein AM+CoCl2+lonomycin fluorescence in the Vehicle group.
Calcein AM1.fcs: The first repeated detection of Calcein AM fluorescence in the Vehicle group.
Calcein AM2.fcs: The second repeated detection of Calcein AM fluorescence in the Vehicle group.
Calcein AM3.fcs: The third repeated detection of Calcein AM fluorescence in the Vehicle group.
Negative1.fcs: The first repeated detection of negative fluorescence in the Vehicle group.
Negative2.fcs: The second repeated detection of negative fluorescence in the Vehicle group.
Negative3.fcs: The third repeated detection of negative fluorescence in the Vehicle group.
3) Sub-folder 3: ROS
Sub-sub-folder1: Mito
Mito1.fcs: The first repeated detection of mitochondrial ROS in the Mito group.
Mito2.fcs: The second repeated detection of mitochondrial ROS in the Mito group.
Mito3.fcs: The third repeated detection of mitochondrial ROS in the Mito group.
Sub-sub-folder2: Negative control
Tube1.fcs: The negative fluorescence detection.
Sub-sub-folder3: NS
NS1.fcs: The first repeated detection of mitochondrial ROS in the NS group.
NS2.fcs: The second repeated detection of mitochondrial ROS in the NS group.
NS3.fcs: The third repeated detection of mitochondrial ROS in the NS group.
Sub-sub-folder4: Sham
Sham1.fcs: The first repeated detection of mitochondrial ROS in the Sham group.
Sham2.fcs: The second repeated detection of mitochondrial ROS in the Sham group.
Sham3.fcs: The third repeated detection of mitochondrial ROS in the Sham group.
Sub-sub-folder5: Vehicle
Vehicle1.fcs: The first repeated detection of mitochondrial ROS in the Vehicle group.
Vehicle2.fcs: The second repeated detection of mitochondrial ROS in the Vehicle group.
Vehicle3.fcs: The third repeated detection of mitochondrial ROS in the Vehicle group.
Links to other publicly accessible locations of the data:
N/a
Code/Software
The FCS file can be opened and analyzed using various bioinformatics tools and software. One of the most common options is FlowJo. Other software options include FCS Express, Kaluza, WinList, and several others.