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Species discovery and delimitation in ground beetles of the subgenus Trepanedoris: matrices and trees

Maddison, David1; Sproul, John2

Published Mar 17, 2026 on Dryad. https://doi.org/10.5061/dryad.9cnp5hqx1

Data files

Mar 17, 2026 version files 5.33 MB

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Abstract

We infer species boundaries in small ground beetles of the genus Bembidion, presenting the discoveries made by a class of students in an experiential learning course at Oregon State University. Focusing on the Bembidion connivens group of subgenus Trepanedorisoutside of California, we integrate data from external structure and male genitalia, DNA sequences of one mitochondrial gene and six nuclear genes, and geographic distribution. We analyze the DNA data using Bayesian multispecies coalescent methods to help infer reproductive communities or species; SPEEDEMON tended to split species more than what our integrated approach inferred, whereas DELINEATE tended to lump more than synthetic inference. We infer 12 species in North America outside of California, including four new species. The files in this archive include a Mesquite NEXUS file containing DNA data matrices and inferred trees, Supplementary Tables including localities of specimens and GenBank accession numbers of analyzed DNA sequences, and Supplementary Figures showing intermediate results in the DELINEATE analysis.