Data from: Simultaneous recording of spikes and calcium signals in odor-evoked responses of Drosophila antennal neurons
Data files
Jul 10, 2026 version files 442.09 KB
-
README.md
27.26 KB
-
Source_data_table_for_Figure_1_non_formatted.xlsx
55.29 KB
-
Source_data_table_for_Figure_1.xlsx
39.15 KB
-
Source_data_table_for_Figure_2_non_formatted.xlsx
35.32 KB
-
Source_data_table_for_Figure_2.xlsx
33.92 KB
-
Source_data_table_for_Figure_3_non_formatted.xlsx
24.74 KB
-
Source_data_table_for_Figure_3.xlsx
24.32 KB
-
Source_data_table_for_Figure_4_non_formatted.xlsx.xlsx
84.23 KB
-
Source_data_table_for_Figure_4.xlsx.xlsx
83.15 KB
-
Source_data_table_for_Figure_S1_non_formatted.xlsx
17.35 KB
-
Source_data_table_for_Figure_S1.xlsx
17.36 KB
Abstract
Most insects, including agricultural pests and disease vectors, rely on olfaction for key innate behaviors. Consequently, there is growing interest in studying insect olfaction to gain insights into odor-driven behavior and to support efforts in vector control. Calcium imaging using GCaMP fluorescence is widely used to identify olfactory receptor neurons (ORNs) responsive to ethologically relevant odors. However, accurate interpretation of GCaMP signals in the antenna requires understanding both response uniformity within an ORN population and how calcium signals relate to spike activity. To address this, we optimized a dual-modality recording method combining single-sensillum electrophysiology and widefield imaging for Drosophila ORNs. Calcium imaging showed that homotypic ab2A neurons exhibit similar odor sensitivity, consistent with spike recordings, indicating that a single ORN’s response can reliably represent its homotypic counterparts. Furthermore, concurrent dual recordings revealed that peak calcium responses are linearly correlated with spike activity, regardless of imaging site (soma or dendrites), GCaMP variant, odorant, or fly age. These findings validate the use of somatic calcium signals as a reliable proxy for spike activity in fly ORNs and provide a foundation for future large-scale surveys of spike–calcium response relationships across diverse ORN types.
Description of the data and file structure
The files contain electrophysiology and calcium imaging data collected through simultaneous recording. The associated figures are indicated in the file name. The formatting in the file is required for improved readability and organization of data. The empty columns/cells contain no data but are required for data organization purposes. Please see below for detailed descriptions per file.
Files and variables
File: Source_data_table_for_Figure_1.xlsx
Description: This Excel file contains source data for Figure 1. The workbook includes raw and normalized calcium-imaging and electrophysiological responses of ab2A (Or59b) ORNs to increasing concentrations of methyl acetate, multiple ab2 sensilla were measured per antenna. The dataset quantifies odor sensitivity using both calcium fluorescence and spike-frequency responses and includes pEC50 measurements for each biological replicate.
Sheet: Ca2+ response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 0.000001 = 10⁻⁶ dilution).
- Fly1–Fly7: Independent biological replicates.
- ROI1–ROIN: Individual regions of interest (ROIs) quantified within each fly. The number of ROIs varies by fly.
- Ca2+ response (ΔF/F, %): Peak odor-evoked calcium response expressed as percent fluorescence change relative to baseline.
- pEC50: Negative logarithm of the odor concentration producing half-maximal calcium response for each ROI. Used as a measure of odor sensitivity.
- ΔpEC50: Range (difference) of pEC50 values among ROIs within a single fly.
- AVE: Mean pEC50 across all ROIs for the indicated fly.
- SEM: Standard error of the mean pEC50 across all ROIs for the indicated fly.
- P value (pEC50 across flies): Statistical comparison of pEC50 distributions across biological replicates using one-way ANOVA.
Sheet: spike response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions.
- Fly1–Fly6: Independent biological replicates.
- n1–n6: Individual ab2 sensillum recorded within each fly.
- Peak spike response (Hz): Maximum odor-evoked firing rate measured by single-sensillum recording (SSR), in spikes per second.
- pEC50: Negative logarithm of the odor concentration producing half-maximal spike response.
- ΔpEC50: Range of pEC50 values across repeated measurements for each fly.
- AVE: Mean pEC50 across technical replicates.
- SEM: Standard error of the mean pEC50 across technical replicates.
- P value (pEC50 across flies): Statistical comparison across biological replicates using one-way ANOVA.
- Sheet: Ca2+ response normalized data
- methyl acetate dilution: Odor stimulus concentration.
- Fly1–Fly7: Biological replicates.
- ROI1–ROIN: Individual regions of interest per fly.
- Normalized calcium response (%): Calcium responses normalized to the maximum response observed within each ROI, where the maximum response is set to 100%.
Sheet: spike response normalized data
Variables
- methyl acetate dilution: Odor stimulus concentration.
- Fly1–Fly6: Biological replicates.
- n1–n6: Individual ab2 sensillum recorded within each fly.
- Normalized spike response (%): Spike responses normalized to the maximum response observed within each recording, where the maximum response is set to 100%.
File: Source_data_table_for_Figure_2.xlsx
Description: This Excel file contains source data for Figure 2. The workbook includes odor-evoked calcium-imaging and electrophysiological recordings from Drosophila ab2A/Or59b olfactory receptor neurons in response to methyl acetate stimulation. The dataset compares manually selected (SSR-recorded) and algorithm-selected calcium-imaging regions of interest (ROIs), quantifies response kinetics, and evaluates the spike-calcium response relationships across different GCaMP variants and imaging locations.
Sheet: Ca2+ response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 0.000001 = 10⁻⁶ dilution).
- Fly1–Fly7: Independent biological replicates.
- ROI1–ROIN: Individual regions of interest (ROIs) measured within each fly. The number of ROIs varies by fly.
- Ca2+ response (ΔF/F, %): Peak odor-evoked calcium fluorescence response expressed as percent change relative to baseline fluorescence.
- Peak ΔF/F (%): Maximum fluorescence response recorded for each ROI.
- Sheet: SSR & algorithm-selected ROIs
- Left table: Peak calcium responses
- SSR-recorded ROI: Peak calcium response (ΔF/F, %) measured at the manually selected ROI corresponding to the sensillum recorded electrophysiologically.
- Algorithm-selected ROI: Peak calcium response (ΔF/F, %) measured at the ROI selected computationally based on maximum response.
- Right table: Sensitivity (pEC50)
- SSR-recorded ROI: pEC50 calculated from manually selected ROIs.
- Algorithm-selected ROI: pEC50 calculated from algorithm-selected ROIs.
- AVE: Mean across flies.
- SEM: Standard error of the mean.
Sheet: Spike and Ca2+ kinetics
Variables
- Time to reach peak response after stimulus onset (ms):
- Spike response: Time in milliseconds from stimulus onset to peak spike-frequency response.
- Ca2+ response (SSR-recorded ROI): Time in milliseconds from stimulus onset to peak calcium fluorescence response.
- Time constant for response rise phase (ms):
- Spike response: Exponential rise constant for spike response.
- Ca2+ response (SSR-recorded ROI): Exponential rise constant for calcium response.
- Time constant for response decay phase (ms):
- Spike response: Exponential decay constant for spike response.
- Ca2+ response (SSR-recorded ROI): Exponential decay constant for calcium response.
Sheet: Associated spike and Ca2+
Experimental groups:
- Or59b>GCaMP6m, dendritic ROI: Calcium signals measured in dendritic compartments using GCaMP6m.
- Or59b>GCaMP6m, somatic ROI: Calcium signals measured in somatic compartments using GCaMP6m.
- Or59b>GCaMP7c, somatic ROI: Somatic calcium signals measured using GCaMP7c.
- Or59b>GCaMP8s, somatic ROI: Somatic calcium signals measured using GCaMP8s.
Variables
- Peak spike response (Hz): Maximum odor-evoked spike-frequency response measured by single-sensillum recording (SSR).
- methyl acetate dilution: Odor concentration.
- Fly1–Fly7: Individual biological replicates.
- AVE: Mean across biological replicates.
- SEM: Standard error of the mean.
Sheet: Statistics
Description: Linear regression analysis
Variables
- GCaMP variants and ROI: Experimental condition analyzed.
- Linear relationship: Best-fit linear regression equation relating calcium response (y) to spike response (x).
- R2 value: Coefficient of determination for the regression fit.
- P value: Statistical significance of the regression.
- One-way MANOVA statistics:
- Group 1 / Group 2: Experimental groups compared.
- P value: Statistical significance of multivariate response-profile comparisons.
File: Source_data_table_for_Figure_4.xlsx.xlsx
Description: This Excel file contains source data for Figure 4. The workbook includes paired electrophysiological and calcium-imaging measurements from Drosophila ab2A/Or59b olfactory receptor neurons from different adult ages (3-day, 5-day, and 7-day old). The dataset was used to evaluate how age affects spike-calcium response relationships and baseline GCaMP fluorescence.
Variables
Common variables in 3-day, 5-day, and 7-day spike and Ca2+ response sheets:
- Peak spike response (Hz): Maximum odor-evoked action potential firing rate measured by single-sensillum recording (SSR), in spikes per second.
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 1e-9 = 10⁻⁹ dilution).
- Fly1–Fly7 (or Fly1–Fly6): Independent biological replicates.
- AVE: Mean response across biological replicates.
- SEM: Standard error of the mean across biological replicates.
- Peak Ca2+ response (ΔF/F, %): Maximum odor-evoked calcium fluorescence response paired with the corresponding spike response.
Sheet: Ca2+ response over time
Variables
For each age group (3-day, 5-day, 7-day):
- Time points relative to stimulus onset (ms): Time in milliseconds relative to odor delivery onset. Negative values indicate pre-stimulus baseline; 0 indicates stimulus onset.
- 10⁻⁹ to 10⁻⁴: Methyl acetate odor concentrations tested.
- Average GCaMP fluorescence signal over time (ΔF/F, %): Mean calcium fluorescence trace across flies at each odor concentration.
Sheet: Linear regression statistics
Variables
For each age group:
- R2: Coefficient of determination for the linear regression between spike response and calcium fluorescence response.
- Linear relationship: Best-fit regression equation, where x is spike response (Hz) and y is calcium response (ΔF/F, %).
- P value: Statistical significance of the regression.
- One-way MANOVA statistics:
- Group 1 / Group 2: Age groups compared.
- P value: Statistical significance of multivariate response-profile comparisons.
Definitions:
- y: Ca2+ fluorescence response (ΔF/F, %)
- x: spike response (Hz)
- Sheet: Basal GCaMP fluorescence
- Fly1–Fly7: Individual biological replicates.
- 3 day old / 5 day old / 7 day old: Baseline GCaMP fluorescence intensity measured before odor stimulation, reported in arbitrary units (AU).
- One-way ANOVA statistics:
- Group 1 / Group 2: Age groups compared.
- P value: Statistical significance of baseline fluorescence differences.
File: Source_data_table_for_Figure_S1.xlsx
Description: This Excel file contains source data for Supplementary Figure S1. The workbook includes unnormalized Ca2+ responses for data shown in Figure 1.
Sheet: Ca2+ response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration, represented as decimal dilution fractions. For example, 0.000001 indicates a 10⁻⁶ dilution.
- Fly1–Fly7: Independent biological replicates. Each fly contains multiple ROIs.
- ROI 1–ROI N: Individual regions of interest measured within each fly. The number of ROIs varies by fly, ranging from 4 to 15 ROIs.
- Peak ΔF/F (%): Peak odor-evoked calcium fluorescence response, expressed as percent change in fluorescence relative to baseline fluorescence.
Sheet: Statistics
Variables
- Two-way ANCOVA: Statistical analysis performed separately for each fly.
- Fly ID: Identifier of the fly analyzed.
- Independent Variable 1: ROI identity, treated as a categorical variable.
- Independent Variable 2: Odorant dilution, treated as a continuous variable.
- P value for Variable 1: P value testing whether ROI identity significantly affects calcium-response measurements within that fly.
File: Source_data_table_for_Figure_3.xlsx
Description: This Excel file contains source data for Figure 3. The workbook includes paired electrophysiological and calcium-imaging responses from Drosophila ab2A/Or59b olfactory receptor neurons expressing different genetically encoded calcium indicators (GCaMP6m, GCaMP7c, and GCaMP8s). Responses were measured for two odorants, methyl acetate and ethyl acetate, across a range of stimulus concentrations.
Variables
Common variables in GCaMP6m, GCaMP7c, and GCaMP8s sheets:
- Left table (methyl acetate):
- Peak spike response (Hz): Maximum odor-evoked action potential firing rate measured by single-sensillum recording (SSR), in spikes per second.
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 0.000001 = 10⁻⁶ dilution).
- Fly1–Fly7: Independent biological replicates.
- AVE: Mean response across biological replicates.
- SEM: Standard error of the mean across biological replicates.
- Right table (ethyl acetate):
- Peak spike response (Hz): Maximum odor-evoked action potential firing rate measured by SSR.
- ethyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions.
- Fly1–Fly6 or Fly1–Fly7: Independent biological replicates, depending on experiment.
- AVE: Mean response across biological replicates.
- SEM: Standard error of the mean across biological replicates.
- Calcium-imaging values:
- Peak Ca2+ fluorescence response (ΔF/F, %): Maximum odor-evoked calcium response measured as percent change in fluorescence relative to baseline. These values are paired with corresponding spike responses for each odor and concentration.
Sheet: Linear regression statistics
Variables
For each GCaMP variant (GCaMP6m, GCaMP7c, GCaMP8s):
- R2: Coefficient of determination for the linear regression between spike response and calcium fluorescence response.
- Linear relationship: Best-fit regression equation, where x is spike response (Hz) and y is calcium fluorescence response (ΔF/F, %).
- P value: Statistical significance of the regression.
- One-way MANOVA statistics:
- Comparison: Multivariate comparison between methyl acetate and ethyl acetate response profiles for each GCaMP variant.
- P value: Statistical significance of the comparison.
- Definitions:
- y: Ca2+ fluorescence response (ΔF/F, %)
- x: spike response (Hz)
File: Source_data_table_for_Figure_1_non_formatted.xlsx
Description: This Excel file contains non-formatted source data for Figure 1. All formatting was removed and empty cells are replaced with NaN to improve accessibility. The workbook includes raw and normalized calcium-imaging and electrophysiological responses of ab2A (Or59b) ORNs to increasing concentrations of methyl acetate, multiple ab2 sensilla were measured per antenna. The dataset quantifies odor sensitivity using both calcium fluorescence and spike-frequency responses and includes pEC50 measurements for each biological replicate.
Sheet: Ca2+ response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 0.000001 = 10⁻⁶ dilution).
- Fly1–Fly7: Independent biological replicates.
- ROI1–ROIN: Individual regions of interest (ROIs) quantified within each fly. The number of ROIs varies by fly.
- Ca2+ response (ΔF/F, %): Peak odor-evoked calcium response expressed as percent fluorescence change relative to baseline.
- pEC50: Negative logarithm of the odor concentration producing half-maximal calcium response for each ROI. Used as a measure of odor sensitivity.
- ΔpEC50: Range (difference) of pEC50 values among ROIs within a single fly.
- AVE: Mean pEC50 across all ROIs for the indicated fly.
- SEM: Standard error of the mean pEC50 across all ROIs for the indicated fly.
- P value (pEC50 across flies): Statistical comparison of pEC50 distributions across biological replicates using one-way ANOVA.
Sheet: spike response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions.
- Fly1–Fly6: Independent biological replicates.
- n1–n6: Individual ab2 sensillum recorded within each fly.
- Peak spike response (Hz): Maximum odor-evoked firing rate measured by single-sensillum recording (SSR), in spikes per second.
- pEC50: Negative logarithm of the odor concentration producing half-maximal spike response.
- ΔpEC50: Range of pEC50 values across repeated measurements for each fly.
- AVE: Mean pEC50 across technical replicates.
- SEM: Standard error of the mean pEC50 across technical replicates.
- P value (pEC50 across flies): Statistical comparison across biological replicates using one-way ANOVA.
Sheet: Ca2+ response normalized data
Variables
- methyl acetate dilution: Odor stimulus concentration.
- Fly1–Fly7: Biological replicates.
- ROI1–ROIN: Individual regions of interest per fly.
- Normalized calcium response (%): Calcium responses normalized to the maximum response observed within each ROI, where the maximum response is set to 100%.
Sheet: spike response normalized data
Variables
- methyl acetate dilution: Odor stimulus concentration.
- Fly1–Fly6: Biological replicates.
- n1–n6: Individual ab2 sensillum recorded within each fly.
- Normalized spike response (%): Spike responses normalized to the maximum response observed within each recording, where the maximum response is set to 100%.
File: Source_data_table_for_Figure_2_non_formatted.xlsx
Description: This Excel file contains non-formatted source data for Figure 2. All formatting was removed and empty cells are replaced with NaN to improve accessibility. The workbook includes odor-evoked calcium-imaging and electrophysiological recordings from Drosophila ab2A/Or59b olfactory receptor neurons in response to methyl acetate stimulation. The dataset compares manually selected (SSR-recorded) and algorithm-selected calcium-imaging regions of interest (ROIs), quantifies response kinetics, and evaluates the spike-calcium response relationships across different GCaMP variants and imaging locations.
Sheet: Ca2+ response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 0.000001 = 10⁻⁶ dilution).
- Fly1–Fly7: Independent biological replicates.
- ROI1–ROIN: Individual regions of interest (ROIs) measured within each fly. The number of ROIs varies by fly.
- Ca2+ response (ΔF/F, %): Peak odor-evoked calcium fluorescence response expressed as percent change relative to baseline fluorescence.
- Peak ΔF/F (%): Maximum fluorescence response recorded for each ROI.
Sheet: SSR & algorithm-selected ROIs
Variables
- Left table: Peak calcium responses
- SSR-recorded ROI: Peak calcium response (ΔF/F, %) measured at the manually selected ROI corresponding to the sensillum recorded electrophysiologically.
- Algorithm-selected ROI: Peak calcium response (ΔF/F, %) measured at the ROI selected computationally based on maximum response.
- Right table: Sensitivity (pEC50)
- SSR-recorded ROI: pEC50 calculated from manually selected ROIs.
- Algorithm-selected ROI: pEC50 calculated from algorithm-selected ROIs.
- AVE: Mean across flies.
- SEM: Standard error of the mean.
Sheet: Spike and Ca2+ kinetics
Variables
- Time to reach peak response after stimulus onset (ms):
- Spike response: Time in milliseconds from stimulus onset to peak spike-frequency response.
- Ca2+ response (SSR-recorded ROI): Time in milliseconds from stimulus onset to peak calcium fluorescence response.
- Time constant for response rise phase (ms):
- Spike response: Exponential rise constant for spike response.
- Ca2+ response (SSR-recorded ROI): Exponential rise constant for calcium response.
- Time constant for response decay phase (ms):
- Spike response: Exponential decay constant for spike response.
- Ca2+ response (SSR-recorded ROI): Exponential decay constant for calcium response.
Sheet: Associated spike and Ca2+
Experimental groups:
Or59b>GCaMP6m, dendritic ROI: Calcium signals measured in dendritic compartments using GCaMP6m.
Or59b>GCaMP6m, dendritic ROI: Calcium signals measured in dendritic compartments using GCaMP6m.
Or59b>GCaMP6m, somatic ROI: Calcium signals measured in somatic compartments using GCaMP6m.
Or59b>GCaMP7c, somatic ROI: Somatic calcium signals measured using GCaMP7c.
Or59b>GCaMP8s, somatic ROI: Somatic calcium signals measured using GCaMP8s.
Variables
- Peak spike response (Hz): Maximum odor-evoked spike-frequency response measured by single-sensillum recording (SSR).
- methyl acetate dilution: Odor concentration.
- Fly1–Fly7: Individual biological replicates.
- AVE: Mean across biological replicates.
- SEM: Standard error of the mean.
Sheet: Statistics
Description: Linear regression analysis
Variables
- GCaMP variants and ROI: Experimental condition analyzed.
- Linear relationship: Best-fit linear regression equation relating calcium response (y) to spike response (x).
- R2 value: Coefficient of determination for the regression fit.
- P value: Statistical significance of the regression.
- One-way MANOVA statistics:
- Group 1 / Group 2: Experimental groups compared.
- P value: Statistical significance of multivariate response-profile comparisons.
File: Source_data_table_for_Figure_4_non_formatted.xlsx.xlsx
Description: This Excel file contains non-formatted source data for Figure 4. All formatting was removed and empty cells are replaced with NaN to improve accessibility. The workbook includes paired electrophysiological and calcium-imaging measurements from Drosophila ab2A/Or59b olfactory receptor neurons from different adult ages (3-day, 5-day, and 7-day old). The dataset was used to evaluate how age affects spike-calcium response relationships and baseline GCaMP fluorescence.
Variables
Common variables in 3-day, 5-day, and 7-day spike and Ca2+ response sheets:
- Peak spike response (Hz): Maximum odor-evoked action potential firing rate measured by single-sensillum recording (SSR), in spikes per second.
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 1e-9 = 10⁻⁹ dilution).
- Fly1–Fly7 (or Fly1–Fly6): Independent biological replicates.
- AVE: Mean response across biological replicates.
- SEM: Standard error of the mean across biological replicates.
- Peak Ca2+ response (ΔF/F, %): Maximum odor-evoked calcium fluorescence response paired with the corresponding spike response.
Sheet: Ca2+ response over time
For each age group (3-day, 5-day, 7-day):
- Time points relative to stimulus onset (ms): Time in milliseconds relative to odor delivery onset. Negative values indicate pre-stimulus baseline; 0 indicates stimulus onset.
- 10⁻⁹ to 10⁻⁴: Methyl acetate odor concentrations tested.
- Average GCaMP fluorescence signal over time (ΔF/F, %): Mean calcium fluorescence trace across flies at each odor concentration.
Sheet: Linear regression statistics
Variables
For each age group:
- R2: Coefficient of determination for the linear regression between spike response and calcium fluorescence response.
- Linear relationship: Best-fit regression equation, where x is spike response (Hz) and y is calcium response (ΔF/F, %).
- P value: Statistical significance of the regression.
- One-way MANOVA statistics:
- Group 1 / Group 2: Age groups compared.
- P value: Statistical significance of multivariate response-profile comparisons.
Definitions:
- y: Ca2+ fluorescence response (ΔF/F, %)
- x: spike response (Hz)
- Sheet: Basal GCaMP fluorescence
- Fly1–Fly7: Individual biological replicates.
- 3 day old / 5 day old / 7 day old: Baseline GCaMP fluorescence intensity measured before odor stimulation, reported in arbitrary units (AU).
- One-way ANOVA statistics:
- Group 1 / Group 2: Age groups compared.
- P value: Statistical significance of baseline fluorescence differences.
File: Source_data_table_for_Figure_S1_non_formatted.xlsx
Description: This Excel file contains non-formatted source data for Figure S1. All formatting was removed and empty cells are replaced with NaN to improve accessibility. The workbook includes unnormalized Ca2+ responses for data shown in Figure 1.
Sheet: Ca2+ response raw data
Variables
- methyl acetate dilution: Odor stimulus concentration, represented as decimal dilution fractions. For example, 0.000001 indicates a 10⁻⁶ dilution.
- Fly1–Fly7: Independent biological replicates. Each fly contains multiple ROIs.
- ROI 1–ROI N: Individual regions of interest measured within each fly. The number of ROIs varies by fly, ranging from 4 to 15 ROIs.
- Peak ΔF/F (%): Peak odor-evoked calcium fluorescence response, expressed as percent change in fluorescence relative to baseline fluorescence.
Sheet: Statistics
Variables
- Two-way ANCOVA: Statistical analysis performed separately for each fly.
- Fly ID: Identifier of the fly analyzed.
- Independent Variable 1: ROI identity, treated as a categorical variable.
- Independent Variable 2: Odorant dilution, treated as a continuous variable.
- P value for Variable 1: P value testing whether ROI identity significantly affects calcium-response measurements within that fly.
File: Source_data_table_for_Figure_3_non_formatted.xlsx
Description: This Excel file contains non-formatted source data for Figure 3. All formatting was removed and empty cells are replaced with NaN to improve accessibility. The workbook includes paired electrophysiological and calcium-imaging responses from Drosophila ab2A/Or59b olfactory receptor neurons expressing different genetically encoded calcium indicators (GCaMP6m, GCaMP7c, and GCaMP8s). Responses were measured for two odorants, methyl acetate and ethyl acetate, across a range of stimulus concentrations.
Variables
Common variables in GCaMP6m, GCaMP7c, and GCaMP8s sheets:
Left table (methyl acetate):
- Peak spike response (Hz): Maximum odor-evoked action potential firing rate measured by single-sensillum recording (SSR), in spikes per second.
- methyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions (e.g., 0.000001 = 10⁻⁶ dilution).
- Fly1–Fly7: Independent biological replicates.
- AVE: Mean response across biological replicates.
- SEM: Standard error of the mean across biological replicates.
Right table (ethyl acetate):
- Peak spike response (Hz): Maximum odor-evoked action potential firing rate measured by SSR.
- ethyl acetate dilution: Odor stimulus concentration represented as decimal dilution fractions.
- Fly1–Fly6 or Fly1–Fly7: Independent biological replicates, depending on experiment.
- AVE: Mean response across biological replicates.
- SEM: Standard error of the mean across biological replicates.
Calcium-imaging values:
- Peak Ca2+ fluorescence response (ΔF/F, %): Maximum odor-evoked calcium response measured as percent change in fluorescence relative to baseline. These values are paired with corresponding spike responses for each odor and concentration.
Sheet: Linear regression statistics
Variables
For each GCaMP variant (GCaMP6m, GCaMP7c, GCaMP8s):
- R2: Coefficient of determination for the linear regression between spike response and calcium fluorescence response.
- Linear relationship: Best-fit regression equation, where x is spike response (Hz) and y is calcium fluorescence response (ΔF/F, %).
- P value: Statistical significance of the regression.
- One-way MANOVA statistics:
- Comparison: Multivariate comparison between methyl acetate and ethyl acetate response profiles for each GCaMP variant.
- P value: Statistical significance of the comparison.
Definitions:
- y: Ca2+ fluorescence response (ΔF/F, %)
- x: spike response (Hz)
Code/software
Microsoft Excel is required to view the uploaded files.