Data from: Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation potential and facilitate tendon regeneration
Data files
Jun 05, 2025 version files 212.76 KB
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fig1e.xlsx
8.81 KB
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fig1g.xlsx
9.02 KB
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fig1h.xlsx
8.80 KB
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fig1j.xlsx
8.75 KB
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fig1k_l.xlsx
12.72 KB
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fig2b.xlsx
9.03 KB
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fig2c.xlsx
12.27 KB
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fig2g.xlsx
9.28 KB
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fig4b.xlsx
9.23 KB
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fig4c.xlsx
12.78 KB
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fig4d.xlsx
13.83 KB
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fig4g.xlsx
8.81 KB
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fig5d.xlsx
9.40 KB
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fig5g.xlsx
8.81 KB
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fig6b.xlsx
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fig6c.xlsx
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fig6d.xlsx
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fig6e.xlsx
8.93 KB
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fig7e.xlsx
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fig7g.xlsx
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README.md
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Abstract
Tendon injury occurs at high frequency and is difficult to repair. Identification of human stem cells being able to regenerate tendon will greatly facilitate the development of regenerative medicine for tendon injury. Genetic and functional analyses identify human CD29⁺/CD56⁺ myogenic progenitors with tenogenic differentiation potential in vitro and in vivo. Transplantation of human CD29+/CD56+ myogenic progenitors contributes to injured tendon repair and thus improves locomotor function. Interestingly, the tendon differentiation potential in mouse muscle stem cells is minimal and the higher TGFβ signaling level may be the key for the distinct feature of human CD29+/CD56+ myogenic progenitors. The discovery of bi-potential CD29⁺/CD56⁺ myogenic progenitors highlight their potential as a novel adult stem cell source for tendon regeneration.
Dataset DOI: 10.5061/dryad.b2rbnzss8
Description of the data and file structure
Data of RT-qPCR results, immunofluorescence staining, bio-mechanical test, force test, and treadmill exercise test.
Files and variables
File: fig1e.xlsx
Description: Proportion of CD29+/CD56+ myogenic progenitors in a specific cell population
Variables
- CD29+/CD56+ myogenic progenitors
- Total CD29+/CD56+ cells
- Total myogenic progenitors
File: fig1g.xlsx
Description: Statistical analysis of the percentage of PAX7+, MYOD1+, and MYF5+ cells in the isolated CD29+/CD56+ myogenic progenitors.
Variables
- PAX7+, MYOD1+ and MYF5+ cells
- DAPI+ cells
File: fig1h.xlsx
Description: Analysis of cell proliferation for human CD29+/CD56+ myogenic progenitors in vitro. 10,000 isolated human CD29+/CD56+ myogenic progenitors were plated for proliferation and counted at each time point.
Variables
- Cell counts at different times.
File: fig1j.xlsx
Description: Percentage of nuclei in MyHC+ myotubes after differentiation of the human CD29+/CD56+ myogenic progenitors.
Variables
- Total DAPI+ cells
- MyHC+ DAPI
File: fig1k_l.xlsx
Description: Relative expression level of PAX7, MYF5, MYOD1, MYOG, MYH1, and MYH3 in human CD29+/CD56+ myogenic progenitors (Undifferentiated) and differentiated myotubes (Differentiated).
Variables
- CT (cycle threshold) value
- Delta Ct value
- Delta Delta Ct value
- fold change
File: fig2b.xlsx
Description: Quantifying TNC and SCX fluorescent intensity in human CD29+/CD56+ myogenic progenitors has undergone myogenic and tenogenic differentiation, respectively.
Variables
- TNC fluorescent intensity
- SCX fluorescent intensity
File: fig2g.xlsx
Description: Statistical analysis of the myogenic and tenogenic differentiation efficiency of human CD29+/CD56+ myogenic progenitors.
Variables
- MyHC-positive wells
- MyHC negative wells
- SCX positive wells
- SCX negative wells
File: fig2c.xlsx
Description: Relative expression levels of genes enriched in tendon cells. RT-qPCR assays were performed with human CD29+/CD56+ myogenic progenitors upon myogenic and tenogenic differentiation, respectively. GAPDH served as a reference gene.
Variables
- CT value
- Relative Quantification (RQ) in fold change
- CT Mean value
File: fig4b.xlsx
Description: Percentage of nuclei in MyHC+ myotubes.
Variables
- MyHC negative nuclei
- Total nuclei
File: fig4c.xlsx
Description: Relative expression levels of myogenic differentiation-specific genes MyoG, Myh1, and Myh3. Mouse MuSCs were induced to differentiate into muscle and tendon, respectively. Mouse MuSCs before and after differentiation, together with primary tenocytes, were harvested and subjected to RT-qPCR analysis. Gapdh served as a reference gene.
Variables
- CT value
- Fold change
File: fig4d.xlsx
Description: Relative expression levels of tenogenic differentiation marker genes Scx, Tnc, Col I, Mkx, and Thbs4. Mouse MuSCs were induced to differentiate into muscle and tendon, respectively. Mouse MuSCs before and after differentiation, together with primary tenocytes, were harvested and subjected to RT-qPCR analysis. Gapdh served as a reference gene.
Variables
- CT value
- Fold change
File: fig4g.xlsx
Description: Percentage of tdTomato+ TNC+ cells 4 months after tendon injury.
Variables
- TNC+ tdTomato+ MyHC- cells
- Total cells
File: fig5g.xlsx
Description: TNC+ tdTomato+ cells in tendon tissue after transplantation of murine MuSCs.
Variables
- TNC+/Tdtomato+/MyHC- cells
- Total cells
File: fig5d.xlsx
Description: Percentage of human cells expressing skeletal muscle marker MyHC or tendon marker TNC after being transplanted to the injured tendon.
Variables
- TNC+ Lamin AC+ cells
- Lamin AC+ cells
- MyHC+ Lamin AC+ cells
- Lamin AC+ cells1
File: fig6b.xlsx
Description: Collagen fibril diameter and percentage of collagen fibril area for the injured tendon with PBS injection, injured tendon with human CD29+/CD56+ myogenic progenitors transplantation, and uninjured tendon.
Variables
- Collagen fibril diameter
- Percentage of collagen fibril area
File: fig6d.xlsx
Description: The results of twitch and tetanus plantarflexion force of the involved limb with PBS injection after tendon injury, human CD29+/CD56+ myogenic progenitors transplantation after tendon injury, and uninjured tendon.
Variables
- Twitch torque
- Tetanus torque
File: fig6e.xlsx
Description: The results of treadmill exercise for tendon-injured mice with or without human CD29+/CD56+ myogenic progenitors transplantation.
Variables
- Endurance time
- Max fatigue speed
File: fig6c.xlsx
Description: The max load and stiffness of the injured tendon with PBS injection, the injured tendon with human CD29+/CD56+ myogenic progenitors transplantation, and the uninjured tendon.
Variables
- Max load
- Stiffness
File: fig7e.xlsx
Description: Relative expression levels of tendon-related genes. RT-qPCR assays were performed with human CD29+/CD56+ myogenic progenitors upon tenogenic differentiation with or without TGFβ signaling pathway inhibitor SB-431542 for 12 days, respectively. GAPDH served as a reference gene.
Variables
- CT value
- Fold change
File: fig7g.xlsx
Description: Relative expression levels of muscle-related genes. RT-qPCR assays were performed with human CD29+/CD56+ myogenic progenitors upon tenogenic differentiation with or without TGFβ signaling pathway inhibitor SB-431542 for 12 days, respectively. GAPDH served as a reference gene.
Variables
- CT value
- Fold change
The differential expression analysis for RNA-seq data between human CD29+/CD56+ myogenic progenitors and mouse MuSCs was performed according to previous literature (PMID: 30925894).
Access information
Other publicly accessible locations of the data:
- NA
Data was derived from the following sources:
- NA