Data from: The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs
Data files
Feb 13, 2026 version files 53.27 KB
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DATA_PONED2528449R2_YuZhang_20260124.xlsx
48.57 KB
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README.md
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Abstract
Drug hypersensitivity reactions (DHR) are commonly observed with various medications. However, some drugs, like Qingkailing (QKL), iodixanol, and vancomycin, previously classified as pseudoallergenic, do not align with clinical observations, creating uncertainty in understanding DHR mechanisms and implementing prevention. In response, our research team hypothesized that IgE-mediated allergy and pseudoallergic hypersensitivity may synergistically lead to more severe reactions. We established an IgE-mediated hypersensitivity model using an anti-dinitrophenyl IgE monoclonal antibody in RBL-2H3 cells and employed ovalbumen (OVA) for guinea pigs. Compound 48/80 served as a positive control for non-immunologic hypersensitivity. We measured β-hexosaminidase and histamine released in cells and guinea pigs to focus on hypersensitivity responses from both IgE-mediated and pseudoallergic pathways. Results indicated that combining pseudoallergenic drugs and IgE significantly increased the hypersensitivity response compared to IgE or separate compound 48/80 groups. This effect was mirrored in the OVA-induced model. Notably, the three pseudoallergic drugs exhibited a similar increase in IgE-mediated hypersensitivity. These findings highlight the additive effects of IgE-mediated and pseudoallergic hypersensitivity, suggesting a more potent DHR response. The study also measured several cytokines and developed a method to differentiate between allergic, pseudoallergic DHRs, and additive reactions, aiding in understanding and controlling serious DHR risks in clinical practice.
Dataset DOI: 10.5061/dryad.bvq83bkpz
Description of the data and file structure
This dataset supports the research article titled "The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs" (PLOS ONE Manuscript ID: PONE-D-25-28449R2), authored by Yu Zhang, Chengbo Zheng, Qilong Xu, Cunyu Li, Yunfeng Zheng, and Guoping Peng.
Files and variables
File: DATA_PONED2528449R2_YuZhang_20260124.xlsx
Description: In vitro experiments were conducted using RBL-2H3 cells, a widely used model for studying allergic responses. The dataset includes key measurements such as cell degranulation rates (assessed by β-hexosaminidase release assay), levels of inflammatory cytokines (e.g., IL-4, TNF-α) detected via ELISA, and cell viability data. These data reflect the response of RBL-2H3 cells to stimuli related to IgE-mediated and pseudoallergic pathways, as well as their combined effects. In vitro experiments were conducted using RBL-2H3 cells, a widely used model for studying allergic responses. The dataset includes key measurements such as cell degranulation rates (assessed by β-hexosaminidase release assay), levels of inflammatory cytokines (e.g., IL-4, TNF-α) detected via ELISA, and cell viability data. These data reflect the response of RBL-2H3 cells to stimuli related to IgE-mediated and pseudoallergic pathways, as well as their combined effects.
In vivo experiments were performed on guinea pigs, which were divided into appropriate groups based on experimental design. The dataset contains physiological and biochemical data from guinea pigs, including changes in body temperature, skin reaction scores (assessing allergic edema and erythema), serum levels of histamine and specific IgE, and pathological observations of relevant tissues. These data provide direct evidence for the additive effect of the two hypersensitivity types in a mammalian model.
All data have been de-identified and processed in compliance with relevant ethical and scientific standards. Detailed experimental protocols, including cell culture conditions, stimulus concentrations, animal handling procedures, and detection methods, are described in the accompanying research article. This dataset is essential for replicating the study's findings and can serve as a valuable resource for other researchers investigating allergic and pseudoallergic mechanisms, as well as their interactions.
This dataset contains the source data for the figures included in the main article, which were generated from both in vitro and in vivo experiments designed to investigate the additive effects of IgE-mediated and pseudoallergic hypersensitivity.
Variables
- Control/Untreated cells/animals (negative control)
- IgE, Cells treated with IgE + DNP-BSA (positive control for allergic activation)
- C48/80, Cells treated with compound 48/80 (positive control for pseudoallergic activation)
- OVA, Guinea pigs sensitized with ovalbumin (OVA)
- QKL/Iodi/Van-treatedCells/animals treated with test compounds (Qingkailing Injection,
Iodixanol, vancomycin) - β-Hex, β-HexosaminidaseLysosomal enzyme,relative rate%
- IgE, Immunoglobulin E,, (ng/ml)
- His, Histamine, relative rate%
- IL-4,Interleukin4,(pg/ml)
- IL-13,Interleukin13, (pg/ml)
- IL-6, Interleukin6, (ng/ml)
- C3a, Complement Component 3, (ng/ml)
- C5a, Complement Component 5, (ng/ml)
- SC5b-9, Terminal Complement Complex, TCC, (ng/ml)
Code/software
The data is stored in Microsoft Excel format (.xlsx) containing 14 worksheets corresponding to different figures (Fig1-Fig10, S1-S6 Fig). Free/open software for viewing: - LibreOffice Calc (version 7.0 or higher) - Apache OpenOffice Calc (version 4.1 or higher) - Google Sheets (latest online version) For advanced analysis: - Python 3.8+ with packages: pandas (1.0+), openpyxl (3.0+), matplotlib/seaborn - Workflow: Read specific sheets using pandas.read_excel(), perform data cleaning/analysis, and reproduce figures with visualization libraries. No additional code or scripts are required for basic data viewing.
Access information
Other publicly accessible locations of the data:
- This dataset is only publicly accessible via Dryad, with no additional copies hosted on other public databases.
Data was derived from the following sources:
- This dataset is original raw data generated directly by the authors through laboratory experiments. No part of this data was derived from pre-existing public datasets or other external sources.