Data from: Interactive effects of experimental ocean acidification and warming on coastal nitrogen cycling
Data files
Jun 04, 2026 version files 64.31 KB
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data_c.csv
28.67 KB
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data_r.csv
26.12 KB
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pH.csv
3.42 KB
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README.md
4.28 KB
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Temp.csv
1.82 KB
Abstract
The effects of climate stressors on coastal systems have been studied across multiple biological scales, often focusing on single environmental variables such as temperature and pH and their influence on community structure, species’ performance and biogeochemical cycles. In contrast, the effects of multiple climate stressors on nutrient cycling remain relatively unknown, especially in coastal systems. To address this knowledge gap, we conducted a factorial field experiment to test the hypothesis that increasing intensity of climate stressors would alter the flux and availability of NO₃⁻ and NH₄⁺ through changes in local biological processes. Data include environmental conditions (temperature, pH), biota (percent cover of consumers and producers), and concentrations and accumulation rates of NO₃⁻ and NH₄⁺ associated with these manipulations, conducted in 20 tide pools on the coast of Southeast Alaska, USA over five months from May to September, 2019.
Dataset DOI: 10.5061/dryad.c2fqz61rv
Description of the data and file structure
Data include measurements of temperature, pH, nitrogen concentrations and accumulation rates, and consumer and producer cover associated with experimental manipulations of temperature (warming) and pH (CO2 addition) in tide pools. Data were collected monthly for five months - May through September - during one daytime low tide and one nighttime low tide each month.
Files and variables
File: data_c.csv
Description: Concentrations of nitrate and ammonium and associated environmental and biotic measurements
Variables
- Year: All values are 2019
- Month: May, June, July, August, September
- Time: Day or Night
- Pool: Identifier for each tide pool
- Heat: 0 = Unheated, 1 = Experimentally heated
- CO2: 0 = Ambient CO2 conditions, 1 = CO2 added
- Treatment: Descriptor of experimental treatment - Control, Heated, CO2_added, Heat+CO2
- Hanna_pH_total_scale: Mean pH measurement (total scale)
- Temp_oC: Mean temperature measurement (degC)
- c_NO3: Mean nitrate concentration (micromolar)
- c_NH4: Mean ammonium concentration (micromolar)
- Prod_Cover: Mean cover of primary producers (%, can total > 100% due to layering)
- Cons_Cover: Mean cover of consumers (%, can total > 100% due to layering)
- pH_SD: Standard deviation of pH measurement
- Temp_SD: Standard deviation of temperature measurement
- c_NO3_SD: Standard deviation of nitrate concentration
- c_NH4_SD: Standard deviation of ammonium concentration
File: data_r.csv
Description: Rates of nitrate and ammonium accumulation and associated environmental and biotic measurements
Variables
- Year: All values are 2019
- Month: May, June, July, August, September
- Time: Day or Night
- Pool: Identifier for each tide pool
- Heat: 0 = Unheated, 1 = Experimentally heated
- CO2: 0 = Ambient CO2 conditions, 1 = CO2 added
- Treatment: Descriptor of experimental treatment - Control, Heated, CO2_added, Heat+CO2
- Hanna_pH_total_scale: Mean pH measurement (total scale)
- Temp_oC: Mean temperature measurement (degC)
- Rate_Nitrate: Mean nitrate accumulation rate (micromoles per Liter per hour)
- Rate_Ammonium: Mean ammonium accumulation rate (micromoles per Liter per hour)
- Prod_Cover: Mean cover of primary producers (%, can total > 100% due to layering)
- Cons_Cover: Mean cover of consumers (%, can total > 100% due to layering)
- pH_SD: Standard deviation of pH measurement
- Temp_SD: Standard deviation of temperature measurement
- c_NO3_SD: Standard deviation of nitrate concentration
File: pH.csv
Description: pH measurements in each tide pool
Variables
- Pool $: Number identifying tide pool
- Trt $: Experimental treatment - Control, Heated, CO2, Both
- time: Day or Night
- T1: May pH values (total scale)
- T2: June pH values (total scale)
- T3: July pH values (total scale)
- T4: August pH values (total scale)
- T5: September pH values (total scale)
- Heat: Experimental warming treatment; 0 = Unheated, 1 = Heated
- CO2: Experimental CO2-addition treatment; 0 = Ambient CO2, 1 = CO2 added
- Mean_pH: Average of monthly pH values for each tide pool during experimental manipulation
- Prev_pH: Average of monthly pH values for each tide pool during the previous, pre-manipulation season
File: Temp.csv
Description: Temperature measurements for tide pools during the manipulated and pre-manipulation seasons
Variables
- T1: May tide pool temperature values (degC)
- T2: June tide pool temperature values (degC)
- T3: July tide pool temperature values (degC)
- T4: August tide pool temperature values (degC)
- T5: September tide pool temperature values (degC)
- Trt: Experimental treatment: Control, Heat, CO2, Both
- Heat: Heat treatment; 0 = Unwarmed, 1 = Warmed
- CO2: CO2 addition treatment; 0 = Ambient CO2, 1 = CO2 added
- Pool: Tide pool number
- Prev: Temperature (degC) during the previous, unmanipulated summer
- Exp_Temp: Temperature (degC) during the experimental manipulation
Code/software
Files are .csv and easily opened using text or spreadsheet software.