Data and code from: High-concentration MEHP triggers mtDNA depletion in undifferentiated HepaRG and C2C12 cultures and disrupts mitochondrial homeostasis in both HepaRG culture states
Data files
May 12, 2026 version files 33.36 KB
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ECAR.Data.D13.Diff.HepaRG.Minus.MEHP.csv
2.14 KB
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ECAR.Data.D13.Diff.HepaRG.Plus.MEHP.csv
1.83 KB
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ECAR.Data.D13.Undiff.HepaRG.Minus.MEHP.csv
1.48 KB
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ECAR.Data.D13.Undiff.HepaRG.Plus.MEHP.csv
2.48 KB
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ECAR.Data.D7.Diff.HepaRG.Minus.MEHP.csv
2.72 KB
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ECAR.Data.D7.Diff.HepaRG.Plus.MEHP.csv
3.05 KB
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OCR.Data.D13.Diff.HepaRG.Minus.MEHP.csv
2.30 KB
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OCR.Data.D13.Diff.HepaRG.Plus.MEHP.csv
1.94 KB
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OCR.Data.D13.Undiff.HepaRG.Minus.MEHP.csv
1.57 KB
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OCR.Data.D13.Undiff.HepaRG.Plus.MEHP.csv
2.67 KB
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OCR.Data.D7.Diff.HepaRG.Minus.MEHP.csv
2.88 KB
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OCR.Data.D7.Diff.HepaRG.Plus.MEHP.csv
3.25 KB
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README.md
5.04 KB
Abstract
This dataset contains bioenergetics data generated from undifferentiated and differentiated HepaRG cell culture models following mono(2-ethylhexyl) phthalate (MEHP) treatment. Data include normalized Seahorse XFp Mito Stress Test measurements, including oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), provided as structured comma-separated value (.csv) files. The dataset includes experimental variables such as treatment condition (MEHP/plus vs. vehicle/minus), cell differentiation state, time point, and normalized quantitative measurements with associated units. These data support analysis of mitochondrial bioenergetics under toxicant exposure conditions and are suitable for reanalysis. Associated experimental outputs, including western blot and Southern blot image data used to assess protein expression and mitochondrial DNA (mtDNA) stability, are available via the linked Zenodo repository. No human subjects data are included. All data are de-identified and shared under CC0 (Dryad), with associated image files available under CC BY (Zenodo).
Dataset DOI: 10.5061/dryad.c59zw3rpr
Description of the data and file structure
Experimental Overview
HepaRG cells (undifferentiated and differentiated) were exposed to 300 µM MEHP or vehicle control for defined durations. Protein and DNA were extracted for western blot and Southern blot analyses. In parallel, mitochondrial function was assessed using Seahorse XFp bioenergetics assays.
All values in this dataset are normalized outputs suitable for downstream statistical analysis.
File Naming Convention
Each Seahorse data file name is made up of a number of components that describe the experimental conditions:
1) Assay Type: Either OCR or ECAR data.
- OCR.data: Oxygen consumption rate data; Units: pmol O₂/min/µg cellular protein
- ECAR.data: Extracellular acidification rate data; Units: mpH/min/µg cellular protein
2) Time point: Either D7 or D13.
- D7: 7 days of MEHP or vehicle treatment.
- D13: 13 days of MEHP or vehicle treatment.
3) Culture State: Either Diff. or Undiff.
- Diff.HepaRG: Differentiated HepaRG cell culture.
- Undiff.HepaRG: Undifferentiated HepaRG cell culture.
4) Treatment: Either Plus.MEHP or Minus.MEHP.
- Plus.MEHP: Cell culture treated with 300 µM mono(2-ethylhexyl) phthalate.
- Minus.MEHP: Vehicle-treated control cell culture.
Internal File Variables
The following variables are listed as columns within each CSV file:
- Time: The specific measurement time point (in minutes) during the Seahorse assay.
- n: Replicate sample wells across Seahorse XFp miniplates (e.g., n1, n2, n3).
File: ECAR.Data.D7.Diff.HepaRG.Minus.MEHP.csv
Description: Normalized ECAR data for differentiated HepaRG cells after 7 days of vehicle control treatment.
File: ECAR.Data.D7.Diff.HepaRG.Plus.MEHP.csv
Description: Normalized ECAR data for differentiated HepaRG cells after 7 days of MEHP treatment.
File: ECAR.Data.D13.Diff.HepaRG.Minus.MEHP.csv
Description: Normalized ECAR data for differentiated HepaRG cells after 13 days of vehicle control treatment.
File: ECAR.Data.D13.Diff.HepaRG.Plus.MEHP.csv
Description: Normalized ECAR data for differentiated HepaRG cells after 13 days of MEHP treatment.
File: ECAR.Data.D13.Undiff.HepaRG.Minus.MEHP.csv
Description: Normalized ECAR data for undifferentiated HepaRG cells after 13 days of vehicle control treatment.
File: ECAR.Data.D13.Undiff.HepaRG.Plus.MEHP.csv
Description: Normalized ECAR data for undifferentiated HepaRG cells after 13 days of MEHP treatment.
File: OCR.Data.D7.Diff.HepaRG.Minus.MEHP.csv
Description: Normalized OCR data for differentiated HepaRG cells after 7 days of vehicle control treatment.
File: OCR.Data.D7.Diff.HepaRG.Plus.MEHP.csv
Description: Normalized OCR data for differentiated HepaRG cells after 7 days of MEHP treatment.
File: OCR.Data.D13.Diff.HepaRG.Minus.MEHP.csv
Description: Normalized OCR data for differentiated HepaRG cells after 13 days of vehicle control treatment.
File: OCR.Data.D13.Diff.HepaRG.Plus.MEHP.csv
Description: Normalized OCR data for differentiated HepaRG cells after 13 days of MEHP treatment.
File: OCR.Data.D13.Undiff.HepaRG.Minus.MEHP.csv
Description: Normalized OCR data for undifferentiated HepaRG cells after 13 days of vehicle control treatment.
File: OCR.Data.D13.Undiff.HepaRG.Plus.MEHP.csv
Description: Normalized OCR data for undifferentiated HepaRG cells after 13 days of MEHP treatment.
Data Relationships and Notation
- Seahorse data correspond to bioenergetic profiles shown in Supplementary Figure 56
- Western blot and Southern blot image data are hosted on Zenodo (see below)
- Quantitative values in the journal article were derived from these normalized datasets
Code/software
No custom scripts are included in this dataset.
Access information
Other publicly accessible locations of the data:
- Supplementary image data are available through the Zenodo repository. These include: western blot images (TCE staining and immunodetection), Southern blot images and Seahorse assay plots.
Data was derived from the following sources:
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All data were generated experimentally as part of the associated study and were not derived from external databases.
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Data were generated and processed using:
- Seahorse Wave Desktop Software (Agilent Technologies)
- Fiji/ImageJ (for band quantification; see Wheeler et al., 2019)
Reference:
Wheeler JH, Young CKJ, Young MJ. 2019. Analysis of Human Mitochondrial DNA Content by Southern Blotting and Nonradioactive Probe Hybridization. Curr Protoc Toxicol. 80(1):e75.