Dataset DOI: 10.5061/dryad.cnp5hqcmd

Description of the data and file structure

This repository contains the raw data, calibration records, and R scripts used to reproduce the statistical analyses and figures presented in Brisco et al. (2026), "Evaluation of ultra-low volume mosquito control field cage trial methods: assessing sentinel cage design, deployment methods, and post-application mosquito handling."

Files and variables

Mosquito population abbreviations:

• CQ1: Laboratory susceptible colony of Culex quinquefasciatus
• WCP: Wild-type colony of Culex pipiens s.l.
• KNWR: Laboratory susceptible colony of Culex tarsalis
• VFWA: Field-collected population of Culex tarsalis
• CSUF: Field-collected population of Culex quinquefasciatus
• KING: Field-collected population of Aedes aegypti
• ROCK: Field-collected population of Aedes aegypti

File: 1_droplet_data.csv

Description: Raw droplet data collected for each trial. These data were used to generate Figure 8 in the associated manuscript.

Variables:

• date: date the trial was conducted
• insecticide: product applied during the trial (DeltaGard, Merus 3.0, Pyronyl 525)
• trial #: unique trial identifier
• transect: transect identifier (A-C)
• distance: distance from the application line (100, 200, 300 ft)
• DV0.5: volume median diameter (µm)
• DV0.9: 90 % of the volume of the spray is in droplets smaller than this value (µm)
• density: drops/mm²
• total_drops: total number of drops included in the analysis

File: 2_equipment_calibration_record.pdf

Description: Droplet analysis data collected during calibration of the application equipment for the Fyfanon-ULV trial conducted in Reedley, CA. This file includes statistical summaries for DV0.5 (µm), DV0.9 (µm), DV0.1 (µm), and total droplet count for two consecutive calibration tests of CMAD’s truck mounted Clarke Grizzly ULV Sprayer #7 applying undiluted Fyfanon-ULV at 0.71 fl oz/acre. Detailed droplet measurements are provided below each calibration summary. Calibration tests were conducted using the DC-IV droplet calibration method.

File: 3_sentinel_cage_mortality.csv

Description: Dataset used for sentinel cage treatment and mosquito transfer analyses.

Variables:

• trial #: unique trial identifier
• date: date the trial was conducted
• transect: transect identifier (A-C)
• distance_ft: distance from the application line (100, 200, 300 ft)
• population: mosquito population tested
• insecticide: DeltaGard ® (Bayer Environmental Science, Cary, NC, USA; 2.0 % deltamethrin), Merus ® 3.0 (Clarke, St. Charles, IL, USA; 5 % pyrethrins), Pyronyl 525 Oil Concentrate (Central Garden & Pet Company, Schaumburg, IL, USA; 5 % pyrethrins, 25 % piperonyl butoxide (PBO))
• cage_type: type of sentinel cage deployed
• deployment_type: fixed = sentinel cage was mounted on a fixed station, vane = cage mounted on a cage vane that positioned the cage to face into the wind
• transfer: Y = mosquitoes were transferred to clean cartons 1-h after exposure, N = mosquitoes were left in sentinel cages that were placed in a ziptop bag with a damp paper towel.
• total_mosquitoes: number of mosquitoes in the cage
• dead_12h: total mosquitoes dead at the 12-h count
• alive_12h: total mosquitoes alive at the 12-h count
• mort%_12h: corrected percent mortality

File: 4_cage_treatment_analysis.R

Description: Performs generalized linear mixed model (GLMM) analysis evaluating the effects of sentinel cage treatment, insecticide, and post-application mosquito transfer on 12-h mosquito mortality and generates Figure 9.

File: 5_transfer_analysis.R

Description: Performs generalized linear mixed model (GLMM) analysis evaluating the effect of post-application mosquito transfer on 12-h mosquito mortality and generates Figure 10.

File: 6_cage_offset_comparison.csv

Description: Dataset used for the sentinel cage offset analysis.

Variables

• trial #: unique trial identifier
• date: date the trial was conducted
• transect: transect identifier (A-C)
• distance_ft: distance from the application line (100, 200, 300 ft)
• population: mosquito population tested
• insecticide: Merus ® 3.0 (Clarke, St. Charles, IL, USA; 5 % pyrethrins), Pyronyl 525 Oil Concentrate (Central Garden & Pet Company, Schaumburg, IL, USA; 5 % pyrethrins, 25 % piperonyl butoxide (PBO))
• cage_type: type of sentinel cage deployed
• deployment_type: sentinel cage deployment method (fixed = sentinel cage mounted on a fixed station; vane = sentinel cage mounted on a cage vane that positioned the cage to face into the wind)
• cage_angle: angle of the sentinel cage relative to the wind direction. A cage facing directly into the wind was assigned an angle of 0°, whereas a cage positioned perpendicular to the wind direction was assigned an angle of 90°.
• total_mosquitoes: number of mosquitoes in the cage
• dead_12h: total mosquitoes dead at the 12-h count
• alive_12h: total mosquitoes alive at the 12-h count
• mort%_12h: corrected 12-h percent mortality

File: 7_cage_offset_analysis.R

Description: Performs generalized linear mixed model (GLMM) analysis evaluating the effects of sentinel cage deployment configuration on 12-h mosquito mortality and generates Figure 11.

Code/software

Analyses were conducted in R version 4.6.1 (released June 24, 2026). The R scripts included in this repository reproduce the statistical analyses and figures reported in the associated manuscript. All scripts are designed to run using the accompanying supplemental datasets located in the same directory.

Required packages

• car
• dplyr
• emmeans
• ggbeeswarm
• ggplot2
• lme4
• readr
• tidyr