Conidia productivity and whitefly insecticidal activity of Beauveria bassiana JEF-507
Data files
Jul 16, 2024 version files 112.17 KB
-
1.Fungal_virulence_screening.xlsx
12.68 KB
-
3a.Quantitative_virulence_of_B._bassiana_isolates.xlsx
10.73 KB
-
3b.Thermotolerance_of_B._bassiana_isolates.xlsx
13.18 KB
-
4.Dose-dependent_insecticidal_activity_of_B._bassiana_JEF-462_and_JEF-507.xlsx
12.96 KB
-
5.Conidial_productivity_on_industrial_grain_substrates.xlsx
11.33 KB
-
6.Thermotolerance_of_conidia_on_grain_substrates.xlsx
19.32 KB
-
7.Insecticidal_efficacy_of_surfactants.xlsx
11.17 KB
-
8.Insecticidal_activity_of_B._bassiana_JEF-462_and_JEF-507_on_pot_condition.xlsx
11.33 KB
-
README.md
9.48 KB
Abstract
Silverleaf whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a destructive insect pest damaging to diverse crops by vectoring several plant pathogenic viruses, which consequently causes economic losses in crop production. As the resistance of whiteflies to chemical insecticides is increasing, this study aims to investigate the potential of entomopathogenic fungi as an alternative. A total of 72 entomopathogenic fungal isolates, collected from soils using Tenebrio molitor larvae as an insect baiting method, were assessed for their virulence against 2nd nymphs of whitefly. Their virulence was assayed by dipping whitefly-infested tomato leaves in fungal conidia suspensions at 1.0 × 107 conidia/ml. Among the tested isolates, two isolates of Beauveria bassiana JEF-462 and JEF-507 showed high virulence. In the assessment of virulence depending on conidia concentrations, the estimated LC50 values for JEF-462 and JEF-507 were similarly 8.7~14.0 × 107 conidia/ml. However, B. bassiana JEF-507 showed higher conidial productivity and thermotolerance on most of tested 12 grain substrates than B. bassiana JEF-462, and millet was the most suitable grain substrate. Additionally, siloxane as a surfactant was able to sufficiently exhibit the insecticidal activity of JEF-507 against whitefly nymphs compared to other surfactants. In a pot-based greenhouse trial, JEF-507 showed higher control efficacy than chemical insecticides, dinotefuran and spinetoram. This work suggests that B. bassiana JEF-507 could be competitively used as a biopesticide to control silverleaf whiteflies whilst overcoming current resistance issues. The JEF-507 isolate has been registered in Korea, 2022 and successfully commercialized as the name of Chongchae-Stop® in this local market to control whitefly and thrips.
GENERAL INFORMATION
-
Title of Dataset: Data from: Conidia productivity and whitefly insecticidal activity of Beauveria bassiana JEF-507
-
Author Information
Corresponding Investigator
Name: Prof. Jae Su Kim
Institution: Jeonbuk national university, Jeonju, Korea
Email: jskim10@jbnu.ac.krCo-investigator 1
Name: Dr Jong-Cheol Kim
Institution: Jeonbuk national university, Jeonju, Korea
Email: jc.kim124@gmail.comCo-investigator 2
Name: Sehyeon Paek
Institution: Jeonbuk national university, Jeonju, Korea
Email: yyy5157@naver.com -
Date of data collection: 2019-2020
-
Geographic location of data collection: Jeonju in Korea
-
Funding sources that supported the collection of the data: Ministry of Agriculture, Food and Rural Affairs (Korea)
DATA & FILE OVERVIEW
-
File List:
File 1 Name: 1.Fungal virulence screening.xlsxFile 2 Name: 3a.Quantitative virulence of B. bassiana isolates.xlsx
File 3 Name: 3b.Thermotolerance of B. bassiana isolates.xlsx
File 4 Name: 4.Dose-dependent insecticidal activity of B. bassiana JEF-462 and JEF-507.xlsx
File 5 Name: 5.Conidial productivity on industrial grain substrates.xlsx
File 6 Name: 6.Thermotolerance of conidia on grain substrates.xlsx
File 7 Name: 7.Insecticidal efficacy of surfactants.xlsx
File 8 Name: 8.Insecticidal activity of B. bassiana JEF-462 and JEF-507 on pot condition.xlsx
DATA-SPECIFIC INFORMATION FOR: 1.Fungal virulence screening.xlsx
- Number of variables: 5
- Number of cases/rows: 74
- Variable List:
Treatment: control, ERL836, JEF-003, JEF-006, JEF-013, JEF-014, JEF-016, JEF-023, JEF-026, JEF-029, JEF-038, JEF-045, JEF-055, JEF-061, JEF-091, JEF-096, JEF-104, JEF-105, JEF-125, JEF-133, JEF-137, JEF-139, JEF-140, JEF-141, JEF-142, JEF-145, JEF-146, JEF-147, JEF-152, JEF-159, JEF-161, JEF-163, JEF-169, JEF-174, JEF-182, JEF-186, JEF-188, JEF-189, JEF-193, JEF-198, JEF-201, JEF-232, JEF-239, JEF-241, JEF-245, JEF-256, JEF-259, JEF-261, JEF-262, JEF-264, JEF-270, JEF-279, JEF-299, JEF-341, JEF-347, JEF-350, JEF-355, JEF-362, JEF-367, JEF-382, JEF-383, JEF-388, JEF-389, JEF-396, JEF-397, JEF-399, JEF-401, JEF-422, JEF-462, JEF-479, JEF-501, JEF-507, JEF-509
DBT: number of survival whitefly Before Treatment; 0-100
Day 3: number of survival whitefly at Day 3 After Treatment; 0-100
Day 4: number of survival whitefly at Day 4 After Treatment; 0-100
Day 5: number of survival whitefly at Day 5 After Treatment; 0-100 - Missing data codes:
None - Abbreviations used:
DBT; Day Before Treatment, Day 3; Day 3 After Treatment, Day 4; Day 4 After Treatment, Day 5; Day 5 After Treatment - Other relevant information:
To select highly virulent isolates against whiteflies among various entomopathogenic fungi, tomato leaves with whitefly nymphs were treated with conidia suspensions (1.0 ×10^7 conidia/ml) of each isolates using the dipping method to assess for insecticidal activity.
DATA-SPECIFIC INFORMATION FOR: 3a.Quantitative virulence of B. bassiana isolates.xlsx
- Number of variables: 6
- Number of cases/rows: 34
- Variable List:
Treatment: N.C., ERL836, JEF-013, JEF-163, JEF-341, JEF-347, JEF-350, JEF-422, JEF-462, JEF-501, JEF-507
Replicate: 3
DBT: number of survival whitefly Before Treatment; 0-100
Day 3: number of survival whitefly at Day 3 After Treatment; 0-100
Day 4: number of survival whitefly at Day 4 After Treatment; 0-100
Day 5: number of survival whitefly at Day 5 After Treatment; 0-100 - Missing data codes:
None - Abbreviations used:
N.C.; Non-treated control, DBT; Day Before Treatment, Day 3; Day 3 After Treatment, Day 4; Day 4 After Treatment, Day 5; Day 5 After Treatment - Other relevant information:
To select highly virulent isolates against whiteflies among various Beauveria bassiana, tomato leaves with whitefly nymphs were treated with conidia suspensions (1.0 ×10^7 conidia/ml) of each isolates using the dipping method to assess for insecticidal activity.
DATA-SPECIFIC INFORMATION FOR: 3b.Thermotolerance of B. bassiana isolates.xlsx
- Number of variables: 4
- Number of cases/rows: 151
- Variable List:
Treatment: ERL836, JEF-013, JEF-163, JEF-341, JEF-347, JEF-350, JEF-422, JEF-462, JEF-501, JEF-507
Heat: Heat treatment time; 0-120 min
Rep: Replicate; 3
Germination rate: Germination rate of conidia after 18 hours of incubation following heat treatment; 0-100 - Missing data codes:
None - Abbreviations used:
none - Other relevant information:
The conidia suspension of Beauveria bassiana was treated at 45 degrees for 0, 30, 60, 90, and 120 minutes, and the germination rate of the conidia was examined to identify strains with high heat stability.
DATA-SPECIFIC INFORMATION FOR: 4.Dose-dependent insecticidal activity of B. bassiana JEF-462 and JEF-507.xlsx
- Number of variables: 4
- Number of cases/rows: 145
- Variable List:
Treatment: N.C., Dinotefuran, Bb JEF-462_5, Bb JEF-462_6, Bb JEF-462_7, JEF-507_5, JEF-507_6, JEF-507_7
Day: Day after treatment; 0-5
Rep: Replicate; 3
Mortality: Mortality of whitefly nymphs; 0-100 - Missing data codes:
None - Abbreviations used:
N.C.; Non-treated control, Bb JEF-462_5; Beauveria bassiana JEF-462 (1.010^5 conidia/ml) treatment, Bb JEF-462_6; Beauveria bassiana JEF-462 (1.0106 conidia/ml) treatment, Bb JEF-462_7; Beauveria bassiana JEF-462 (1.0*107 conidia/ml) treatment, JEF-507_5; Beauveria bassiana JEF-507 (1.010^5 conidia/ml) treatment, JEF-507_6; Beauveria bassiana JEF-507 (1.0106 conidia/ml) treatment, JEF-507_7; Beauveria bassiana JEF-507 (1.0*107 conidia/ml) treatment - Other relevant information:
The two isolates (Beauveria bassiana JEF-462 and JEF-507) were treated at different concentrations (1.0*10^5,6,7 conidia/ml) on whitefly nymphs on tomato leaves using the dipping method to evaluate their insecticidal activity.
DATA-SPECIFIC INFORMATION FOR: 5.Conidial productivity on industrial grain substrates.xlsx
- Number of variables: 7
- Number of cases/rows: 73
- Variable List:
Grain: Cassia seed, Millet, Oatmeal, Mung beans, Perilla seed, Buckwheat, Barley, Sorghum, Rice, Italian millet, Sesame, Brown rice
Isolate: Bb JEF-462 (Beauveria bassiana JEF-462), Bb JEF-507
Rep: Replicate; 3
Conida: Number of conidia (x10^7 /g) - Missing data codes:
None - Abbreviations used:
Conida: Number of conidia (x10^7 /g) - Other relevant information:
The two isolates (Beauveria bassiana JEF-462 and JEF-507) were cultured on 12 different grain substrates to determine conidia productivity.
DATA-SPECIFIC INFORMATION FOR: 6.Thermotolerance of conidia on grain substrates.xlsx
- Number of variables: 5
- Number of cases/rows: 361
- Variable List:
Grain: Cassia seed, Millet, Oatmeal, Mung beans, Perilla seed, Buckwheat, Barley, Sorghum, Rice, Italian millet, Sesame, Brown rice
Isolate: Bb JEF-462, Bb JEF-507
Heat: Heat treatment time; 0-120 min
Rep: Replicate; 3
Germination rate: Germination rate of conidia after 18 hours of incubation following heat treatment; 0-100 - Missing data codes:
None - Abbreviations used:
Bb; Beauveria bassiana - Other relevant information:
The conidia obtained by culturing the two isolates (Beauveria bassiana JEF-462 and JEF-507) on 12 different grain substrates were heat-treated at 45 degrees for 0-120 minutes, then incubated for 18 hours to determine the germination rate of the conidia and evaluate heat stability.
DATA-SPECIFIC INFORMATION FOR: 7.Insecticidal efficacy of surfactants.xlsx
- Number of variables: 4
- Number of cases/rows: 55
- Variable List:
Surfactant: S-1, S-3, LE-1, LE-3, CO-1, CO-2
Treatment: N.C., Bb JEF-462, Bb JEF-507
Rep: Replicate; 3
Mortality: Mortality of whitefly nymphs; 0-100 - Missing data codes:
None - Abbreviations used:
N.C.; Non-treated Control S-1; 0.01% siloxane, S-3; 0.03% siloxane, LE-1; 0.01% polyoxyethylene-7-lauryl ether, LE-3; 0.03% polyoxyethylene-7-lauryl ether, CO-1; 0.01% polyoxyethylene-12-castorethe, CO-3; 0.03% polyoxyethylene-12-castorethe - Other relevant information:
Three surfactants were diluted at two different concentrations, and the conidia suspensions (1.0*10^7 conidia/ml) of the two isolates (Beauveria bassiana JEF-462 and JEF-507) were prepared with each surfactant to evaluate insecticidal activity against whitefly nymphs.
DATA-SPECIFIC INFORMATION FOR: 8.Insecticidal activity of B. bassiana JEF-462 and JEF-507 on pot condition.xlsx
- Number of variables: 4
- Number of cases/rows: 76
- Variable List:
Treatment: N.C., Bb JEF-462, Bb JEF-507
Day: Day after treatment; 0-8
Rep: Replicate; 3
Mortality: Mortality of whitefly nymphs; 0-100 - Missing data codes:
None - Abbreviations used:
N.C.; Non-treated Control, Bb JEF-462; Beauveria bassiana JEF-462, Bb JEF-507; Beauveria bassiana JEF-507 - Other relevant information:
The conidia suspensions of the two isolates (Beauveria bassiana JEF-462 and JEF-507) were prepared to evaluate insecticidal activity in comparison with chemical insecticides. The conidia suspensions were prepared at a concentration of 1.0*10^7 conidia/ml, and the chemical insecticide was diluted to the recommended concentration. The solutions were then sprayed on tomato plants with whitefly nymphs to assess insecticidal activity.