Ex-vivo MRI of human brain hemispheres from patients with globular glial tauopathy and one typical control
Data files
Feb 28, 2026 version files 2.27 GB
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README.md
1.80 KB
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sub-patient1_acq-280um_echo-3_FLASH.nii.gz
67.70 MB
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sub-patient2_acq-160um_echo-2_FLASH.nii.gz
330.35 MB
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sub-patient3_acq-160um_echo-2_FLASH.nii.gz
483.38 MB
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sub-patient4_acq-160um_echo-2_FLASH.nii.gz
560.08 MB
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sub-patient5_acq-160um_echo-2_FLASH.nii.gz
416.68 MB
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sub-patient6_acq-160um_echo-2_FLASH.nii.gz
411.91 MB
Abstract
Ex vivo whole-hemisphere T2*-weighted MRI of 6 human brain hemispheres, 5 from patients with globular glial tauopathy (GGT) and 1 from a typically aging patient. MRI data is stored as NIFTI files, with one file per hemisphere. Gradient echo times were chosen to be approximately 20 ms to maximize sensitivity to GGT pathology.
Dataset DOI: 10.5061/dryad.dbrv15fgk
Description of the data and file structure
For questions related to this dataset, please contact Dylan Tisdall (mtisdall@pennmedicine.upenn.edu).
Image data is in NIFTI format, organized with one file per patient. Imaging data dimensions are described in the NIFTI data headers. Details of the specific imaging parameters, as well as the patients' clinical and demographic information are included in the associated manuscript.
Files and variables
File: sub-patient1_acq-280um_echo-3_FLASH.nii.gz
Description: NIFTI file of ex vivo MRI for patient 1, 280 um isotropic resolution
File: sub-patient2_acq-160um_echo-2_FLASH.nii.gz
Description: NIFTI file of ex vivo MRI for patient 2, 160 um isotropic resolution
File: sub-patient3_acq-160um_echo-2_FLASH.nii.gz
Description: NIFTI file of ex vivo MRI for patient 3, 160 um isotropic resolution
File: sub-patient4_acq-160um_echo-2_FLASH.nii.gz
Description: NIFTI file of ex vivo MRI for patient 4, 160 um isotropic resolution
File: sub-patient5_acq-160um_echo-2_FLASH.nii.gz
Description: NIFTI file of ex vivo MRI for patient 5, 160 um isotropic resolution
File: sub-patient6_acq-160um_echo-2_FLASH.nii.gz
Description: NIFTI file of ex vivo MRI for patient 6, 160 um isotropic resolution
Human subjects data
Consent to publish de-identified data was received as part of the research protocol approved by the University of Pennsylvania's IRB. All PII has been removed from image headers, and no identifying information is included in the associated manuscript.
Samples were placed in Fomblin (California Vacuum Technology; Freemont, CA), a proton-free fluid with volume magnetic susceptibility close to that of tissue. Samples were enclosed in either custom-build cylindrical holders or plastic bags, and then left to rest for at least two days to allow air bubbles to escape from the tissue. Depending on their size, samples were scanned using either a custom-built birdcage coil or a custom-modified quadrature birdcage (Varian, Palo Alto, CA, USA) coil. These transmit/receive coils were attached to a two-channel transmit-receive adapter (Stark Contrast, Erlangen, Germany). The smallest coil was chosen that could hold each sample, tuned/matched by hand, and then placed into our whole-body 7 T scanner (MAGNETOM Terra, Siemens Healthineers, Erlangen, Germany) using plastic shims under the coils to position the sample near isocenter.
MRI data were acquired with a 3D-encoded, multi-echo gradient-recalled echo (GRE) sequence with non-selective RF pulses. The final echo was followed by an additional completely rephrased readout to measure frequency drifts. Scans for individual patients were acquired with somewhat different scanning protocols, reflecting the evolution of standard practices at our imaging center. Specific details of the scanning parameters for each patient are listed in the associated journal article:
Clinicoanatomic localization of iron-rich gliosis in aphasic presentations of globular glial tauopathy, Brain Communications, in press
Images were reconstructed using the vendor’s on-scanner reconstruction software which automatically corrected the global frequency drift, combined the signal averages in k-space, and produced magnitude images for each echo.