Data from: Ethological profiling defines pain behaviors and parses analgesia from drug side effects in a mouse model of complex regional pain syndrome
Data files
Mar 30, 2026 version files 246.96 KB
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Muwanga_Sourcedata_FIG2-8.xlsx
52.11 KB
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Muwanga_Sourcedata_SUPPFIG1-24.xlsx
166.31 KB
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README.md
28.54 KB
Abstract
Complex regional pain syndrome (CRPS) is a form of chronic post-injury pain affecting the extremities with contributions from the somatic and sympathetic nervous systems. The mouse tibial fracture-cast model was developed to enable preclinical study of CRPS mechanisms and guide condition-specific drug development. Given the inherent limitations of reflex pain measures in mice, we sought to holistically characterize pain-like behaviors in this model in neutral and aversive environments using quantitative behavior analysis with LabGym, a user-friendly automation tool that requires no special equipment or extensive computational resources. Here, we show that tibial fracture-cast injury causes distinct changes in naturalistic and nocifensive behaviors in male and female mice in neutral and aversive environments, demonstrating reliable behavioral categorization using robust learning-based holistic assessment. As proof-of-concept for therapeutic testing, we leveraged this behavioral evaluation to characterize the peripheral versus central effects of targeting alpha-2 receptors (α2-AR) with dexmedetomidine (DEX), a selective α2-AR agonist with analgesic, sedative and anxiolytic properties. We found that DEX reduced mechanical allodynia primarily via central α2-ARs. DEX also decreased motion metrics, grooming and rearing in an open field, and distinctly affected the quality and quantity of grooming in an aversive environment, and systemic α2-AR antagonists did not suppress this effect. Importantly, we also determined that the sedative effects of DEX were attenuated in injured compared to uninjured mice, consistent with known sympathetic nervous system activation in CRPS. Overall, this study highlights the use of automated behavioral testing to parse analgesia from sedation in a unique preclinical pain model.
Dataset DOI: 10.5061/dryad.dncjsxmd3
Description of the data and file structure
These files contain raw data generated and analyzed for the paper entitled, "Ethological profiling defines pain behaviors and parses analgesia from drug side effects in a mouse model of complex regional pain syndrome."
Files and variables
File: Muwanga_Sourcedata_FIG2-8.xlsx
Description: This Excel file contains raw data for the main figures 2-8.
Variables
- Tab 1: Figure 2
- 2b) Mechanical sensitivity before and after tibial fracture-cast injury in male mice
- 2c) Thermal sensitivity before and after tibial fracture-cast injury in male mice
- 2d) Weight bearing before and after tibial fracture-cast injury in male mice
- 2e) Paw width before and after tibial fracture-cast injury in male mice
- 2f) Paw temperature before and after tibial fracture-cast injury in male mice
- 2i) Distance traveled in an open field before and after tibial fracture-cast injury in male mice
- 2j) Average speed in an open field before and after tibial fracture-cast injury in male mice
- 2k) Time spent in the center zone in an open field before and after tibial fracture-cast injury in male mice
- 2l) Time spent in the thigmotaxis zone in an open field before and after tibial fracture-cast injury in male mice
- 2m) Time spent immobile in an open field before and after tibial fracture-cast injury in male mice
- Tab 2: Figure 3
- 3c) Face grooming count, duration, intensity and vigor in an open field before and after tibial fracture-cast injury in male mice
- 3d) Body grooming count, duration, intensity and vigor in an open field before and after tibial fracture-cast injury in male mice
- 3e) Walking count, duration, intensity and vigor in an open field before and after tibial fracture-cast injury in male mice
- 3f) Rearing count, duration, intensity and vigor in an open field before and after tibial fracture-cast injury in male mice
- Tab 3: Figure 4
- 4c) Rearing count, duration, intensity and vigor on a hotplate before and after tibial fracture-cast injury in male mice
- 4d) Grooming count, duration, intensity and vigor on a hotplate before and after tibial fracture-cast injury in male mice
- 4e) Paw licking count, duration, intensity and vigor on a hotplate before and after tibial fracture-cast injury in male mice
- 4f) Paw withdrawal count, duration, intensity and vigor on a hotplate before and after tibial fracture-cast injury in male mice
- Tab 4: Figure 5b-e
- 5b) Mechanical sensitivity before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 30 minutes, 60 minutes, 90 minutes and 120 minutes after DEX treatment in male mice
- 5c) Weight bearing before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 60 minutes and 120 minutes after DEX treatment in male mice
- 5d) Paw width three weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 60 minutes and 120 minutes after DEX treatment in male mice
- 5e) Paw temperature three weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 60 minutes and 120 minutes after DEX treatment in male mice
- Tab 5: Figure 5g and i
- 5g) Mechanical sensitivity before tibial fracture-cast injury and 3 weeks after injury before treatment with saline, DEX alone or an alpha-2 antagonist alone (Yohimbine (YOH); BRL44408 maleate; MK467) and 30 minutes, 60 minutes, 90 minutes and 120 minutes after saline, DEX or alpha-2 antagonist treatment in male mice
- 5i) Mechanical sensitivity before tibial fracture-cast injury and 3 weeks after injury before treatment with saline, DEX alone or an alpha-2 antagonist+DEX (YOH+DEX; BRL44408 maleate:DEX; MK467+DEX) and 30 minutes, 60 minutes, 90 minutes and 120 minutes after saline, DEX or alpha-2 antagonist+DEX treatment in male mice
- Tab 6: Figure 6
- 6c) Face grooming count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in male mice
- 6d) Body grooming count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in male mice
- 6e) Walking count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in male mice
- 6f) Rearing count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in male mice
- Tab 7: Figure 7
- 7c) Grooming count on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 7d) Grooming duration on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 7e) Grooming latency on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 7f) Grooming intensity on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 7g) Grooming vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 7h) Paw licking latency on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- Tab 8: Figure 8
- 8b) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in male mice
- 8c) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in male mice
- 8d) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in male mice
- 8e) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in male mice
- 8f) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in male mice
- 8g) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in male mice
**Each column represents a mouse and missing values are indicated as blank cells
*Intensity is calculated as the accumulated proportional changes of the animal body area between frames divided by the time window for categorizing the behaviors when performing a behavior (Hu et al. 2023)
*Vigor is calculated as the magnitude of the behavior change divided by the time that such a change takes place. Magnitude is the maximum proportional change in animal body area when performing a behavior (Hu et al. 2023)
File: Muwanga_Sourcedata_SUPPFIG1-24.xlsx
Description: This Excel file contains raw data for the supplemental figures 1-24.
Variables
- Tab 1: Supplemental Figure 1
- 1a) Precision, recall, and F1 scores for open field analysis model identification of idling behavior for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 1b) Precision, recall, and F1 scores for open field analysis model identification of walking for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 1c) 1a) Precision, recall, and F1 scores for open field analysis model identification of rearing for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 1d) Precision, recall, and F1 scores for open field analysis model identification of body grooming for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 1e) Precision, recall, and F1 scores for open field analysis model identification of face grooming for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- Tab 2: Supplemental Figure 2
- 2a) Walking, body grooming, face grooming, and rearing latencies in an open field before and after tibial fracture-cast injury in male mice
- 2b) Idle count, duration, latency, intensity and vigor in an open field before and after tibial fracture-cast injury in male mice
- Tab 3: Supplemental Figure 3
- 3b) Face grooming count, duration, intensity, vigor and latency in an open field before and after tibial fracture-cast injury in female mice
- 3c) Body grooming count, duration, intensity, vigor and latency in an open field before and after tibial fracture-cast injury in female mice
- 3d) Walking count, duration, intensity, vigor and latency in an open field before and after tibial fracture-cast injury in female mice
- 3e) Rearing count, duration, intensity, vigor and latency in an open field before and after tibial fracture-cast injury in female mice
- Tab 4: Supplemental Figure 4
- 4a) Precision, recall, and F1 scores for hotplate analysis model identification of miscellaneous behaviors (other) for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 4b) Precision, recall, and F1 scores for hotplate analysis model identification of grooming for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 4c) 1a) Precision, recall, and F1 scores for hotplate analysis model identification of rearing for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 4d) Precision, recall, and F1 scores for hotplate analysis model identification of paw withdrawal for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- 4e) Precision, recall, and F1 scores for hotplate analysis model identification of paw licking for an uninjured (naïve) mice dataset, an injured mice dataset, and a mixed (injured and uninjured) mice dataset
- Tab 5: Supplemental Figure 5
- 5a) Rearing, grooming, paw licking, and paw withdrawal latencies on a hotplate before and after tibial fracture-cast injury in male mice
- 5b) Other (miscellaneous behaviors) count, duration, latency, intensity and vigor on a hotplate before and after tibial fracture-cast injury in male mice
- Tab 6: Supplemental Figure 6
- 6b) Rearing count, duration, intensity, vigor and latency on a hotplate before and after tibial fracture-cast injury in female mice
- 6c) Grooming count, duration, intensity, vigor and latency on a hotplate before and after tibial fracture-cast injury in female mice
- 6d) Paw licking count, duration, intensity, vigor and latency on a hotplate before and after tibial fracture-cast injury in female mice
- 6e) Paw withdrawal count, duration, intensity, vigor and latency on a hotplate before and after tibial fracture-cast injury in female mice
- Tab 7: Supplemental Figure 7
- 7b) Mechanical sensitivity before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 30 minutes, 60 minutes, 90 minutes and 120 minutes after saline or DEX treatment in female mice
- 7c) Mechanical sensitivity before saline or dexmedetomidine (DEX) treatment and 30 minutes, 60 minutes, 90 minutes and 120 minutes after saline or DEX treatment in uninjured male and female mice
- 7d) Mechanical sensitivity as a percentage of baseline before DEX treatment and 30 minutes, 60 minutes, 90 minutes and 120 minutes after DEX treatment in injured male and female mice
- 7f) Mechanical sensitivity before tibial fracture-cast injury and 3 weeks after injury before treatment with saline, DEX alone or an alpha-2 antagonist+DEX (YOH+DEX; BRL44408 maleate:DEX; MK467+DEX) and 30 minutes, 60 minutes, 90 minutes and 120 minutes after saline, DEX or alpha-2 antagonist+DEX treatment in female mice
- Tab 8: Supplemental Figure 8
- 8d) Distance traveled in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after saline or DEX treatment in male mice
- 8e) Average speed in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after saline or DEX treatment in male mice
- 8f) Time spent in the center zone in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after saline or DEX treatment in male mice
- 8g) Time spent in the thigmotaxis zone in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after saline or DEX treatment in male mice
- 8h) Time spent immobile in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before saline or dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after saline or DEX treatment in male mice
- Tab 9: Supplemental Figure 9
- 9b) Distance traveled in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 9c) Average speed in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 9d) Time spent immobile in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 9e) Time spent in the center zone in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 9f) Time spent in the thigmotaxis zone in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- Tab 10: Supplemental Figure 10
- 10b) Distance traveled in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- 10c) Average speed in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- 10d) Time spent in the center zone in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- 10e) Time spent in the thigmotaxis zone in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- 10f) Time spent immobile in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- 10g) Distance traveled in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 10h) Average speed in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 10i) Time spent in the center zone in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 10j) Time spent in the thigmotaxis zone in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- 10k) Time spent immobile in an open field before DEX treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in female mice
- Tab 11: Supplemental Figure 11
- 11b) Face grooming count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured male and injured male mice
- 11c) Body grooming count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured male and injured male mice
- 11d) Walking count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured male and injured male mice
- 11e) Rearing count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured male and injured male mice
- Tab 12: Supplemental Figure 12
- 12a) Face grooming, body grooming, walking, and rearing latencies in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- 12b) Idle count, duration, and latency in an open field before tibial fracture-cast injury and 3 weeks after tibial fracture-cast injury before dexmedetomidine (DEX) treatment and 1 hr up to 1 hr 30 minutes after DEX treatment in male mice
- Tab 13: Supplemental Figure 13
- 13b) Face grooming count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in female mice
- 13c) Body grooming count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in female mice
- 13d) Walking count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in female mice
- 13e) Rearing count, duration, intensity and vigor in an open field before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) DEX treatment in female mice
- Tab 14: Supplemental Figure 14
- 14b) Face grooming count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured female and injured female mice
- 14c) Body grooming count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured female and injured female mice
- 14d) Walking count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured female and injured female mice
- 14e) Rearing count, duration, intensity and vigor in an open field before and after DEX treatment in uninjured female and injured female mice
- Tab 15: Supplemental Figure 15
- 15a) Other (miscellaneous behaviors) count, duration, latency, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 15b) Rearing count, duration, latency, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 15c) Paw withdrawal count, duration, latency, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- 15d) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in male mice
- Tab 16: Supplemental Figure 16
- 16b) Paw withdrawal count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured male and injured male mice
- 16c) Paw licking count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured male and injured male mice
- 16d) Grooming count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured male and injured male mice
- 16e) Rearing count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured male and injured male mice
- Tab 17: Supplemental Figure 17
- 17b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in female mice
- 17c) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in female mice
- 17d) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in female mice
- 17e) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury before (pre-drug) and after (post-drug) saline or DEX treatment in female mice
- Tab 18: Supplemental Figure 18
- 18b) Paw withdrawal count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured female and injured female mice
- 18c) Paw licking count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured female and injured female mice
- 18d) Grooming count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured female and injured female mice
- 18e) Rearing count, duration, intensity and vigor on a hotplate before and after DEX treatment in uninjured female and injured female mice
- Tab 19: Supplemental Figure 19
- 19b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in male mice
- 19c) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in male mice
- 19d) Other (miscellaneous behaviors) count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in male mice
- Tab 20: Supplemental Figure 20
- 20b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in male mice
- 20c) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in male mice
- 20d) Other (miscellaneous behaviors) count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in male mice
- Tab 21: Supplemental Figure 21
- 21b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in male mice
- 21c) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in male mice
- 21d) Other (miscellaneous behaviors) count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in male mice
- Tab 22: Supplemental Figure 22
- 22b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in female mice
- 22c) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in female mice
- 22d) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in female mice
- 22e) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in female mice
- 22f) Other (miscellaneous behaviors) count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or YOH+DEX treatment in female mice
- Tab 23: Supplemental Figure 23
- 23b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in female mice
- 23c) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in female mice
- 23d) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in female mice
- 23e) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in female mice
- 23f) Other (miscellaneous behaviors) count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or BRL+DEX treatment in female mice
- Tab 24: Supplemental Figure 24
- 24b) Paw withdrawal count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in female mice
- 24c) Paw licking count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in female mice
- 24d) Grooming count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in female mice
- 24e) Rearing count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in female mice
- 24f) Other (miscellaneous behaviors) count, duration, intensity and vigor on a hotplate before tibial fracture-cast injury and 3 weeks after injury after DEX or MK467+DEX treatment in female mice
**Each column represents a mouse and missing values are indicated as blank cells
*Intensity is calculated as the accumulated proportional changes of the animal body area between frames divided by the time window for categorizing the behaviors when performing a behavior (Hu et al. 2023)
*Vigor is calculated as the magnitude of the behavior change divided by the time that such a change takes place. Magnitude is the maximum proportional change in animal body area when performing a behavior (Hu et al. 2023)
Code/software
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