Data from: TipQuant: A robust algorithm for quantitative analysis of spatiotemporally dynamic activities in tip-growing cells
Data files
Jul 10, 2026 version files 203.05 MB
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Fig2A_raw_video.tif
18.80 MB
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Fig2D_PT_AtLURE_video.tif
16.73 MB
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Fig4A_ROI_A_YFP-RABA4d.tif
3.46 MB
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Fig4B_ROI_B_CamelliA_CytR.tif
7.40 MB
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Fig4D_F_G_CamelliA_PmG.tif
3.67 MB
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Fig5_PT1_CamelliA_PmR.tif
5.19 MB
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Fig5_PT1_CRIB4_GFP.tif
5.19 MB
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README.md
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SupplementalFigS1_PT10_CamelliA_PmR.tif
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SupplementalFigS1_PT10_CRIB4_GFP.tif
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SupplementalFigS1_PT11_CamelliA_PmR.tif
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SupplementalFigS1_PT11_CRIB4_GFP.tif
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SupplementalFigS1_PT12_CamelliA_PmR.tif
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SupplementalFigS1_PT12_CRIB4_GFP.tif
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SupplementalFigS1_PT2_CamelliA_PmR.tif
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SupplementalFigS1_PT2_CRIB4_GFP.tif
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SupplementalFigS1_PT3_CamelliA_PmR.tif
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SupplementalFigS1_PT3_CRIB4_GFP.tif
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SupplementalFigS1_PT4_CamelliA_PmR.tif
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SupplementalFigS1_PT4_CRIB4_GFP.tif
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SupplementalFigS1_PT5_CamelliA_PmR.tif
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SupplementalFigS1_PT5_CRIB4_GFP.tif
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SupplementalFigS1_PT6_CamelliA_PmR.tif
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SupplementalFigS1_PT6_CRIB4_GFP.tif
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SupplementalFigS1_PT7_CamelliA_PmR.tif
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SupplementalFigS1_PT7_CRIB4_GFP.tif
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SupplementalFigS1_PT8_CamelliA_PmR.tif
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SupplementalFigS1_PT8_CRIB4_GFP.tif
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SupplementalFigS1_PT9_CamelliA_PmR.tif
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SupplementalFigS1_PT9_CRIB4_GFP.tif
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Abstract
Understanding the spatiotemporal dynamics of structural and signaling molecules is essential for elucidating the mechanisms and functions of cell polarity. However, manual quantification of these dynamics is extremely time-consuming, hindering progress in the field. Here, we present TipQuant, an automated analysis tool that robustly detects tips and analyzes the spatiotemporal dynamics of fluorescently labeled molecules and structures on plasma membranes and in the cytoplasm at the apices of tip-growing cells, enabling quantitative analysis of signaling and structural components in these systems. This data deposit includes all video files used to generate the figures.
Dataset DOI: 10.5061/dryad.dr7sqvbd2
Description of the data and file structure
Tip-growing cells, including Arabidopsis pollen tubes and Fusarium graminearum hyphae, expressing various fluorescent reporters, were imaged using confocal microscopy. The videos were then analyzed with TipQuant for automated quantification of intensity distributions and temporal changes on the plasma membrane or within user-defined cytosolic regions.
File: Fig5_PT1_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channel
File: Fig5_PT1_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channel
File: SupplementalFigS1_PT2_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channel (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT2_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channel (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT3_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channel (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT3_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channel (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT5_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channel (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT4_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT5_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT4_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT7_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT6_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT7_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT6_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT10_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT9_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT10_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT8_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT11_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT9_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT11_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT12_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT8_CamelliA_PmR.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CamelliA_PmR channels (biological replicates). Additional data related to Figure 5.
File: SupplementalFigS1_PT12_CRIB4_GFP.tif
Description: Simultaneous analysis of ROP activity and plasma membrane Ca2+ influx in pollen tubes, monitored using CRIB4-GFP channels (biological replicates). Additional data related to Figure 5.
File: Fig2D_PT_AtLURE_video.tif
Description: Raw video (tif format) used to generate Figure 2D. . Tip detection of a CRIB4-GFP pollen tube showing frequent turning behaviors attracted by manually applied pollen tube attractant AtLUREI.
File: Fig2A_raw_video.tif
Description: Raw video (tif format) used to generate Figure 2A.
File: Fig4A_ROI_A_YFP-RABA4d.tif
Description: Raw video (tif format) used to generate Figure 4A. TipQuant-generated mean fluorescence intensity changes with time in cytosolic ROI-A
File: Fig4B_ROI_B_CamelliA_CytR.tif
Description: Raw video (tif format) used to generate Figure 4B. TipQuant-generated mean fluorescence intensity changes with time in cytosolic ROI-B
File: Fig4D_F_G_CamelliA_PmG.tif
Description: Raw video (tif format) used to generate Figure 4D, F, G. Validation of TipQuant-generated kymographs.
Code/software
We developed the TipQuant algorithm (accessible through https://github.com/julianlegouic/TipQuant/) in the associated manuscript for automated tip detection and analysis of the spatiotemporal dynamics of fluorescently labeled molecules and structures on plasma membranes and in the cytoplasm at the apices of tip-growing cells.
Access information
Other publicly accessible locations of the data:
- N/A
Data were derived from the following sources:
- N/A