Dataset DOI: 10.5061/dryad.dr7sqvbfb

Description of the data and file structure

A red tide developed along the shores of La Jolla, CA, in October 2024, continuing through May 2025. Twice-weekly net tow collections were obtained from waters off the Scripps Institution of Oceanography Pier and were subsampled for analysis of Pulse Amplitude Modulated (PAM) Fluorescence to study the fast transient signature of the microbial population.

PAM measurements were carried out using a Photosynthetic System Instruments (PSI; Drásov, Czech Republic) FL-6000F. Measurements were obtained using a factory-supplied program for “OJIP Kinetics”, but modified to have a 3-second duration with a continuous actinic irradiation of 2220 µmol photons m^-2 s^-1 daylight white light and a progressive logarithmic sampling routine. A 3 mL subsample was measured into a 1 cm² polystyrene cuvette that was placed in the fluorometer sample chamber. For the Dark samples, all processing and measurements were made in a dark room; for the Exposed samples, room lights were on at all times. Individual txt files for all sample days of this study are presented.

Sample collection occurred 09/16/2024 - 07/10/2025 (mm/dd/yyyy). Raw data is represented asa  *.txt file for each sample date, with metadata regarding instrumentation, settings, etc. in the multi-line header. File naming structure is "Pier" followed by "DCMU" anand/orLight" then by date (YYYYMMDD), followed (if present) by note (i.e., "red tide").

In each file, Header A includes (TTAB-separatedvalues) for each sample: name, date, time, instrument settings. Header B includes (TTAB-separatedvalues) for each sample: machine-specificoperating parameters.

For each file, the data matrix consists of TAB separated values for (1) first column, Time given in seconds, followed by 10 columns each for "Pier #" (20 minute + dark acclimation), "Pier_DCMU #" (if present, DCMU addition to dark acclimated samples), and "Pier_Light #" (if present, 20 minutes exposure to bright light). Fluorescence values are given in mV.

The files provided are:

• Pier_DCMU_20240916.txt 962.4 KB
• Pier_DCMU_20240923.txt 962.8 KB
• Pier_DCMU_20240926.txt 962.16 KB
• Pier_DCMU_20240930.txt 963.32 KB
• Pier_DCMU_20241003.txt 1.15 MB
• Pier_DCMU_20241007.txt 962.85 KB
• Pier_DCMU_20241014_red_tide_begin.txt 963.16 KB
• Pier_DCMU_20241017.txt 963.75 KB
• Pier_DCMU_20241028_red.txt 962.91 KB
• Pier_DCMU_20241104_red.txt 963 KB
• Pier_DCMU_20241107_red.txt 962.58 KB
• Pier_DCMU_20241111_red.txt 1.42 MB
• Pier_DCMU_Light_20241118_red.txt 1.42 MB
• Pier_DCMU_Light_20241121_red.txt 1.42 MB
• Pier_DCMU_Light_20241125_post_red.txt 1.42 MB
• Pier_DCMU_Light_20241127_post.txt 1.47 MB
• Pier_DCMU_Light_20241202_post.txt 1.42 MB
• Pier_DCMU_Light_20241205_post.txt 1.42 MB
• Pier_DCMU_Light_20241209_post.txt 1.42 MB
• Pier_DCMU_Light_20241212_post.txt 1.47 MB
• Pier_DCMU_Light_20241216_post.txt 1.42 MB
• Pier_DCMU_Light_20241219_post.txt 1.42 MB
• Pier_DCMU_Light_20241223_post.txt 1.42 MB
• Pier_DCMU_Light_20241226_red.txt 1.42 MB
• Pier_DCMU_Light_20241230_red.txt 1.42 MB
• Pier_DCMU_Light_20250102_post.txt 1.42 MB
• Pier_DCMU_Light_20250106_post.txt 1.42 MB
• Pier_DCMU_Light_20250109_post.txt 1.42 MB
• Pier_DCMU_Light_20250113_red.txt 1.42 MB
• Pier_DCMU_Light_20250116_red.txt 1.42 MB
• Pier_DCMU_Light_20250121_red.txt 1.42 MB
• Pier_DCMU_Light_20250123_red.txt 1.42 MB
• Pier_DCMU_Light_20250127.txt 1.42 MB
• Pier_DCMU_Light_20250203.txt 1.42 MB
• Pier_DCMU_Light_20250210.txt 1.42 MB
• Pier_DCMU_Light_20250213.txt 1.42 MB
• Pier_DCMU_Light_20250218-diatom.txt 1.42 MB
• Pier_DCMU_Light_20250220-diatom.txt 1.42 MB
• Pier_DCMU_Light_20250224.txt 1.42 MB
• Pier_DCMU_Light_20250227.txt 1.42 MB
• Pier_DCMU_Light_20250306.txt 1.42 MB
• Pier_DCMU_Light_20250310.txt 1.42 MB
• Pier_DCMU_Light_20250313.txt 1.42 MB
• Pier_DCMU_Light_20250317.txt 1.42 MB
• Pier_DCMU_Light_20250320.txt 1.42 MB
• Pier_DCMU_Light_20250324-red_tide.txt 1.42 MB
• Pier_DCMU_Light_20250331-red_tide.txt 1.42 MB
• Pier_DCMU_Light_20250403.txt 1.42 MB
• Pier_DCMU_Light_20250407.txt 1.42 MB
• Pier_DCMU_Light_20250410.txt 1.42 MB
• Pier_DCMU_Light_20250414.txt 1.42 MB
• Pier_DCMU_Light_20250417.txt 1.42 MB
• Pier_DCMU_Light_20250421_Psn.txt 1.42 MB
• Pier_DCMU_Light_20250424_Psn.txt 1.42 MB
• Pier_DCMU_Light_20250428_Psn.txt 1.42 MB
• Pier_DCMU_Light_20250505.txt 1.42 MB
• Pier_DCMU_Light_20250508.txt 1.42 MB
• Pier_DCMU_Light_20250512.txt 1.42 MB
• Pier_DCMU_Light_20250602.txt 1.42 MB
• Pier_DCMU_Light_20250605.txt 1.42 MB
• Pier_DCMU_Light_20250609-Red.txt 1.42 MB
• Pier_DCMU_Light_20250612.txt 1.42 MB
• Pier_DCMU_Light_20250616.txt 1.42 MB
• Pier_DCMU_Light_20250618.txt 1.42 MB
• Pier_DCMU_Light_20250623.txt 1.42 MB
• Pier_DCMU_Light_20250626.txt 1.42 MB
• Pier_DCMU_Light_20250703.txt 1.42 MB
• Pier_DCMU_Light_20250707.txt 1.42 MB
• Pier_DCMU_Light_20250710.txt 1.38 MB

Code/software

A text file reader is required to read the data.