Dataset DOI: 10.5061/dryad.f1vhhmhcb

Description of the data and file structure

Fecal DNA Metabarcoding Read Data.

Files:

• JVB3405-23S-read-data.csv
• JVB3405-UniCOI-read-data_(1).csv

The columns on the left contain taxonomic information collected from submitting the sequences as bioinformatic queries to the to NCBI GenBank database. The columns that follow are the numbers of reads that match that particular sequence query in each row for the animal represented by the column. The data presented in each column represents an individual animal. Some of the data set cells for the taxonomical information are blank. This is due to the fact that some amplicons contain sequences that cannot identify the source of DNA down to the species level, and thus lower taxonomic information is left blank.

JVB3405-UniCOI-read-data_(1).csv does not have column headers. The data is organized as follows.

Column A contains the Test ID, or the probe used to detect the reads. UniCOI was used for invertebrates for the JBV3405-UniCOI-read-data file, and JBV3405-23S-read-data was used to detect plant  DNA.

Columns B contains the read ID, and column C contains the read DNA sequence detected. 

Columns D-J contain bioinformatics data concerning the origin of the fecal DNA detected, and identify the Kingdom, Phylum, Class, Order, Family, Genus and Species, respectively.

Column K indicates the % sequence match to that found in the NCBI GenBank database.

The data in column K identifies common names for the tax identified.

Column M indicates the number of species in the lowest identified taxonomic level for a specific read.

Columns N-DB contain the number of times that each specific read was detected in the amplicon for the fecal sample from each animal tested.

Code/software

MS Excel

Access information

Other publicly accessible locations of the data:

• N/A

Data was derived from the following sources:

• N/A