In silicon samples for testing the SPrOUT pipeline. We selected 30 species from the Kew Garden Tree of Life database (Zuntini et al., 2024). Reads for all 30 species reads were generated via target capture arrays (with PAFTOL or equivalent methods marked on Tree of Life Explorer). The selection aimed to cover major angiosperm clades and maximize lineage coverage. We created insilicon test datasets from the 30 species. We generated 100 samples of artificial mixed reads, each consisting of mixed reads from three, six, or ten species in equal proportion.