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EEG Recordings for: Optogenetic stimulation of the dorsal striatum bidirectionally controls seizures

Hyder, Safwan1; Lazarini-Lopes, Willian1; Toib, Jonathan1; Williams, Gabrielle1; Sukharev, Alexander1; Forcelli, Patrick1

Research facility: Georgetown University Medical Center
Published Apr 18, 2025 on Dryad. https://doi.org/10.5061/dryad.f7m0cfz6w

Data files

Apr 18, 2025 version files 19.19 GB

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Abstract

Engagement of the basal ganglia experimental seizures was first observed almost 75 years ago. However, the role of the basal ganglia’s input nucleus, the striatum, in seizure control is relatively understudied. To address this gap, we used an optogenetic approach to activate and inactivate neurons in the dorsal striatum of rats submitted to the gamma-butyrolactone (GBL) model of absence epilepsy, amygdala kindling model of temporal lobe epilepsy, and pilocarpine-induced Status Epilepticus (SE).  Open-loop (continuous light delivery) optogenetic activation of  dorsal striatal neurons robustly suppressed seizures in all models. By contrast, open-loop optogenetic silencing increased absence seizure expression and facilitated SE onset but had no effect on kindled seizures. In the GBL model, we also tested the effects of closed-loop modulation (light delivery in response to seizure detection). Closed-loop activation reduced duration of spike-wave discharges (SWDs), while closed-loop inhibition increased SWD duration. This dataset includes electroencephalographic recordings for each subject and test session in the manuscript are included as european data format (.edf) files.  These results demonstrated previously unrecognized anti-absence effects associated with striatal neuromodulation. These findings demonstrate a robust, bidirectional role of the dorsal striatum in the control of multiple seizure types, suggesting that the striatum is a site that can exert broad-spectrum control of seizures.