https://doi.org/10.5061/dryad.fbg79cp62

Description of the data and file structure

Chloroplast genomic DNA was extracted from collected silica-dried leaves (Narzary et al. 2015) using the Ezup Spin Column Super Plant Genomic DNA Extraction Kit (Sangon Biotech, Shanghai). Four chloroplast DNA regions (matK, rbcL, trnL-F and psbA-trnH) were subjected to PCR amplification. The PCR reaction system (25 µl) consisted of 15 µl of 2 Taq Master Mix buffer, 10 µl of ddH2O, 3 µl of the primer mixture (1.5 µl each of the positive and reverse primer), and 2 µl of the DNA template. The parameters for PCR procedure was 95 for 3 min, followed by 40 cycles of 95 for 30 s, appropriate annealing temperature for 30 s, 72 for 1 min, and 10 min for final extension at 72 , the annealing temperatures was 54 for matK and rbcL genes, 49 for trnL-F and psbA-trnH intergenic spacers. 5 µl of the products were tested using electrophoresis in 1% agarose gel at 150 V, 110 mA for 10 to 20 min. San Prep Column DNA Gel Extraction Kit (Sangon Biotech, Shanghai) was used for recovery and purification, then the purified products were sequenced by Sangon Biotech (Shanghai, China – https://www.sangon.com/).

Files and variables

File: Rawdata.fasta

Description: Total 23 sequences of 5 DNA regeions (matK, rbcL, trnLF and psbAtrnH) from 5 different species of genus Artabotrys (Artabotrys fragrans, Artabotrys hainanensis, Artabotrys pachypetalus, Artabotrys pilosus, and Artabotrys rubriflorus).