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Data from: A field deployable chemical reaction for colourimetric and fluorescence detection of atrazine in soil

Taemaitree, Lapatrada1; Charoensakkhachon, Wananya1

Published Mar 12, 2026 on Dryad. https://doi.org/10.5061/dryad.fbg79cp91

Data files

Mar 12, 2026 version files 234.38 MB

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Abstract

Atrazine is a member of the chloro-s-triazine family of pesticides. It is widely used but also tightly regulated due to its potential adverse effects on human health and the environment. This dataset shows the steps towards and the final optimised conditions of a König chemical reaction specific to chloro-s-triazines in solution and on Whatman paper using barbituric acid. This reaction generates a purple colour and pink fluorescence from a colourless initial state. Solution-based reactions are quantified using UV-vis or fluorescence spectrophotometers. Paper-based reactions are observed by the naked eye, with fluorescence changes also visible by eye using an LED torch. Spectrophotometer data is in CSV format, where the first column is the wavelength in nm, and subsequent columns are the absorbance or fluorescence values in arbitrary units at a specific concentration of atrazine in ug/ml (values specified in the column header first row). Data was recorded using a Shimadzu UV-1800 spectrophotometer for UV-vis spectra and using an Edinburgh Instruments FS5 spectrometer for fluorescence spectra. Photographs were taken of the colour changes and fluorescence under a DARKBEAM UV torch (365–370 nm LED, 20 W power, 480 mW radiant intensity) using a iPhone XS Max. This data represents the raw data that makes figures in a peer reviewed publication. As such the data is grouped by figure folders where all data within the folder represent related data to demonstrate an optimisation process or optimal condition results. Figures that reuse data indicate so using text files.