Soil microbial communities regulate soil carbon feedbacks to climate warming through microbial respiration (i.e. metabolic rate). A thorough understanding of the responses of composition, biomass and metabolic rate of soil microbial community to warming is crucial to predict soil carbon stocks in a future warmer climate. Therefore, we conducted a field manipulative experiment in a semi-arid grassland on the Loess Plateau of China to evaluate the responses of the soil microbial community to increased temperature from April 2015 to December 2017. Soil temperature was 2.0 ^oC higher relative to the ambient when open-top chambers (OTCs) were used. Warming did not affect microbial biomass or the composition of microbial functional groups. However, warming significantly decreased microbial respiration, directly resulting from soil pH decrease driven by the co-mediation of aboveground biomass increase, inorganic nitrogen increase and moisture decrease. These findings highlight that the soil microbial community structure of semi-arid grasslands resisted the short-term warming by 2 ^oC, although its metabolic rate declined.

Manipulative Experiment