Next generation sequence counts and enrichment scores for an E. coli Rop deep mutational scanning library after cell survival selection
Data files
Aug 05, 2026 version files 100.78 KB
-
README.md
3.83 KB
-
Rop_Deep_Mutational_Scanning_Counts_Round_0.xlsx
15.73 KB
-
Rop_Deep_Mutational_Scanning_Counts_Round_3.xlsx
15.68 KB
-
Rop_Deep_Mutational_Scanning_Counts_Round_6.xlsx
15.58 KB
-
Rop_Deep_Mutational_Scanning_Scores_Round_3.xlsx
25.51 KB
-
Rop_Deep_Mutational_Scanning_Scores_Round_6.xlsx
24.44 KB
Abstract
Rop is a 63 amino acid, homo-dimeric, four-helix bundle protein that controls the copy number of ColE1 plasmids in E. coli. We measured the overall biological fitness of every possible single point mutant of Rop using a cell growth selection and Illumina next generation sequencing. The datasets report the sequence counts and calculated enrichment scores for each single point mutant in the deep mutational scanning library. Sequence counts are shown for the naive (round 0) library along with the library after three and six rounds of selections. Enrichment scores normalized to the wildtype sequence abundance changes are shown for each single point mutant after three and six rounds of selection.
Dataset DOI: 10.5061/dryad.ffbg79d9w
Description of the data and file structure
These Excel sheets contain the next generation sequencing counts and scores for all Rop single-point mutants in a deep mutational scanning library. Sequence counts are included for the constructed naive libraries and for the mutant pools after rounds of growing cells under fitness selection conditions.
Files and variables
File: Rop_Deep_Mutational_Scanning_Counts_Round_0.xlsx
Description: This file contains next generation sequencing counts for the Rop mutational scanning library. Data is included for the naive (round 0) plasmid pool. The variable descriptions are below. All values have units of counts.
Variables
- Amino Acid: The amino acid present at the randomized position.
- T2, K3, Q4, E5, etc.: The randomized positions. Letter is the wildtype amino acid. Number is the position of the amino acid in the protein sequence.
File: Rop_Deep_Mutational_Scanning_Counts_Round_3.xlsx
Description: This file contains next generation sequencing counts for the Rop mutational scanning library. Data is included for the round 3 plasmid pool. The variable descriptions are below. All values have units of counts.
Variables
- Amino Acid: The amino acid present at the randomized position.
- T2, K3, Q4, E5, etc.: The randomized positions. Letter is the wildtype amino acid. Number is the position of the amino acid in the protein sequence.
File: Rop_Deep_Mutational_Scanning_Counts_Round_6.xlsx
Description: This file contains next generation sequencing counts for the Rop mutational scanning library. Data is included for the round 6 plasmid pool. The variable descriptions are below. All values have units of counts.
Variables
- Amino Acid: The amino acid present at the randomized position.
- T2, K3, Q4, E5, etc.: The randomized positions. Letter is the wildtype amino acid. Number is the position of the amino acid in the protein sequence.
File: Rop_Deep_Mutational_Scanning_Scores_Round_3.xlsx
Description: This file contains next generation sequencing calculated log2-fold enrichment scores for the Rop mutational scanning library. Data is included for the round 3 plasmid pool. The variable descriptions are below. All values are unitless. Log2-fold enrichment scores were unable to be calculated for any single-point mutant with zero sequence counts in the round 0, 3, and/or 6 plasmid pools. The missing enrichment scores are represented by "NA".
Variables
- Amino Acid: The amino acid present at the randomized position.
- T2, K3, Q4, E5, etc.: The randomized positions. Letter is the wildtype amino acid. Number is the position of the amino acid in the protein sequence.
File: Rop_Deep_Mutational_Scanning_Scores_Round_6.xlsx
Description: This file contains next generation sequencing calculated log2-fold enrichment scores for the Rop mutational scanning library. Data is included for the round 6 plasmid pool. The variable descriptions are below. All values are unitless. Log2-fold enrichment scores were unable to be calculated for any single-point mutant with zero sequence counts in the round 0, 3, and/or 6 plasmid pools. The missing enrichment scores are represented by "NA".
Variables
- Amino Acid: The amino acid present at the randomized position.
- T2, K3, Q4, E5, etc.: The randomized positions. Letter is the wildtype amino acid. Number is the position of the amino acid in the protein sequence.
Code/software
Excel or other tabular data software is needed to view the data.