Data from: "A reduced representation libraries approach for nuclear marker development via 454 sequencing applied on Tetramorium (Hymenoptera: Formicidae)" in Genomic Resources Notes Accepted 1 February 2015 to 31 March 2015
Data files
Apr 28, 2015 version files 29.05 MB
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alignments.zip
4.91 MB
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contigs+singletons.fasta.zip
23.13 MB
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distance table.csv
997.35 KB
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scripts+protocol.zip
7.31 KB
Abstract
To clarify species boundaries in the cryptic Tetramorium caespitum/impurum ant species complex, a multi-disciplinary approach is necessary. Nuclear DNA markers constitute one important step in species delimitation. We established a reduced representation library (RRL) based on amplified fragment-length polymorphism (AFLP) PCR products of four species. Amplicons were size-selected by gel-electrophoresis, DNA-fragments recovered from the agarose gels, and 454 libraries constructed by a commercial provider. Pyrosequencing yielded 490,155 reads, from which we designed cross-species amplifying primer pairs for 28 loci. Wetlab procedures, bioinformatic pipeline, alignment statistics, and the developed primers are presented.