Data from: Downregulation of sST2, a decoy receptor for interleukin-33, enhances subcutaneous tumor growth in murine pancreatic cancer cells

Akimoto, Miho 1 ; Koshikawa, Nobuko2; Morinaga, Takao2; Tamamori-Adachi, Mimi1; Takatori, Atsushi2; Takenaga, Keizo2

Published Dec 29, 2025 on Dryad. https://doi.org/10.5061/dryad.g1jwstr2n

Data files

Dec 29, 2025 version files 15.24 KB

Ct_table.csv

2.02 KB
Delta_Ct_table.csv

2.42 KB
Gene_table.csv

7.87 KB
README.md

2.94 KB

Abstract

Despite the recent scientific advancements, pancreatic cancer remains the 7^th leading cause of cancer-related mortality. Pancreatic cancer progression is closely associated with inflammation, and we previously showed that short hairpin RNA–mediated knockdown of sST2 expression, a soluble decoy receptor for the proinflammatory molecule interleukin-33 (IL-33), in mouse Panc02 pancreatic cancer cells reduced malignant growth following pancreatic (orthotopic) implantation. Furthermore, this growth suppression was accompanied by decreased tumor angiogenesis, reduced expression of the neutrophil chemoattractant CXCL3, and a lower number of tumor-associated neutrophils (TANs). In contrast to previous results, in this study, we showed that IL-33-dependent tumor growth and pulmonary metastasis occurred following subcutaneous (ectopic) implantation of sST2-knockdown cells. This was associated with a decrease in the levels of the anti-inflammatory molecule adiponectin and the number of GLUT4-positive cancer-associated adipocytes, as well as an increase in IκBα phosphorylation levels, Cxcl3 expression, and the accumulation of infiltrating CD206⁺ protumor N2 TANs. Taken together, these results suggest that Panc02 cell-derived sST2 differentially affects malignant growth in the tumor microenvironment depending on the implantation site.

https://doi.org/10.5061/dryad.g1jwstr2n

This dataset corresponds to Fig. S4 of the manuscript titled "Downregulation of sST2, a decoy receptor for interleukin-33, enhances subcutaneous tumor growth in murine pancreatic cancer cells." Fig. S4 presents the cytokine and chemokine gene expression profiles obtained using a PCR array in Panc02 tumors with or without sST2 knockdown. The submitted files include:

Gene_table.csv: Primer positions and gene information used in the PCR array, including Unigene and RefSeq IDs, gene symbols, descriptions, and official gene names.

Ct_table.csv: Raw cycle threshold (Ct) values detected for each gene in control (shCont) and sST2-knockdown (sh#5) Panc02 tumors.

Delta_Ct_table.csv: Normalized Ct values using Gapdh as the internal control, and ΔΔCt values representing gene expression changes between shCont and sh#5 groups.

Description of the data and file structure

Gene_table

Position: Location of each primer on the 96-well PCR array plate.
Unigene: Cluster ID from the Unigene database, representing transcripts derived from the same gene. Some wells (H06–H12) contain experimental controls and therefore have no corresponding Unigene ID; these were indicated as N/A.
Refseq: Reference sequence ID provided by NCBI (https://www.ncbi.nlm.nih.gov/).
Symbol: Abbreviated gene symbol.
Description: Brief description of the gene’s function or characteristics.
Gene name: Official full gene name.

Ct_table

Position: Well location on the 96-well PCR array plate.
Symbol: Abbreviated gene symbol.
shCont (Ct): Ct value detected in PCR array using Panc02 mouse pancreatic tumors expressing control shRNA (non-sST2-deficient). Ct values above 35 are considered undetectable and are marked as ND.
sh#5 (Ct): Ct value detected in PCR array using Panc02 mouse pancreatic tumors with sST2 knockdown via shRNA.

Delta_Ct_table

Position: Location on the 96-well PCR array plate.
Symbol: Abbreviated gene symbol.
shCont (Ct(GOI) - Ct(Gapdh)): Normalized Ct value for the gene of interest (GOI) in Panc02 tumors expressing control shRNA (non-sST2-deficient), using Gapdh as the internal control. N/A indicates that the Ct value was undetectable and normalization could not be performed.
sh#5 (Ct(GOI) - Ct(Gapdh)): Normalized Ct value for the GOI in Panc02 tumors with sST2 knockdown.
Delta(Ct): Difference between normalized Ct values in shCont and sh#5 groups. This reflects the change in gene expression in Panc02 tumors due to sST2 deficiency. N/A indicates that the Ct value was undetectable and the calculation could not be performed.