Data from: The lifelong effects of anoxia hormesis in solitary bees
Data files
Jul 08, 2025 version files 125.12 KB
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README.md
2.78 KB
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Wilkinson_and_Lopez-Martinez_data.xlsx
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Abstract
The stimulatory and protective response known as hormesis elicits an often over compensatory response resulting in exposure survival and life history trait improvements. There are an array of abiotic and biotic agents that have been shown to trigger hormesis; most commonly studied are chemicals, temperature, and low oxygen. Investigations into low-oxygen exposures that activate the hormetic response date back nearly 50 years. This body of work reveals that insect performance is dramatically improved by single short low-oxygen events while focusing on the transitory nature of the improvements. Few reports examine whether the effect is longer lasting or lifelong. We previously reported that one hour of anoxia was enough to induce this hormetic response in the alfalfa leafcutting bee, Megachile rotundata (Hymenoptera: Megachilidae). Here we investigated whether that response is transitory or long-lasting by looking at starvation resistance, flight, and locomotory activity throughout the life of the bees. Additionally, we studied the effects of anoxia hormesis on reproduction, diapause incidence, and F1 offspring survival, development, and flight. Anoxia hormesis has lifelong positive effects for the flight of males and females. We also recorded higher starvation survival in bees that experienced hormesis. This improvement in performance came at a steep reproductive cost. However, no costs or benefits were passed to the next generation as we recorded no delays in development, no effects on F1 diapause, no hormesis-related deaths, and no changes in flight performance. We hypothesize that using anoxia hormesis in the context of pollination services by this species, should result in bees that are potentially more active in the field; increasing the numbers of visits to flowers throughout their entire life.
This Wilkinson and Lopez-Martinez README.txt file was generated on 2024-11-5 by Giancarlo Lopez-Martinez
GENERAL INFORMATION
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Title of Dataset: The lifelong effects of anoxia hormesis in solitary bees
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Author Information
Corresponding Investigator
Name: Dr Giancarlo Lopez-Martinez
Institution: North Dakota State University
Email: giancarlo.lopez@ndsu.eduCo-investigator 1
Name: Michaelyne Wilkinson
Institution: New Mexico State University -
Date of data collection: 2021-2022
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Geographic location of data collection: Fargo, ND
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Funding sources that supported the collection of the data: National Science Foundation of the USA
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Recommended citation for this dataset:
DATA & FILE OVERVIEW
- Description of dataset
This is the accompanying dataset to a publication titled: The lifelong effects of anoxia hormesis in solitary bees (DOI: 10.1093/ee/nvaf013). The variables in this data set are Nx: normoxia, which refers to a normal atmospheric oxygen content of ~21% and Ax: anoxia, which refers to an oxygen concentration of zero (0%).
File List: File 1 Name: Wilkinson_and_Lopez-Martinez_data.xlsx
File 1 Description: All the raw data used in this manuscript. The first tab includes a summary of the data for each figure, which is also below.
- 1A) Daily male starvation survival after anoxia hormesis (number of bees alive/treatment).
- 1B) Male starvation survival 48 hours after heat treatment (presented as day of death/bee/age/treatment).
- 1C) Daily female starvation survival after anoxia hormesis (number of bees alive/treatment).
- 1D) Female starvation survival 48 hours after heat treatment (presented as day of death/bee/age/treatment).
- 2A) Male flight ability by age (number of flying bees).
- 2B) Male flight by percent that flew first.
- 2C) Female flight ability by age (number of flying bees).
- 2D) Female flight by the percentage that flew first.
- 3A) Bee daily activity following treatment (average activity/minute).
- 3B) Bee morning activity following treatment (average activity/minute).
- 3C) Bee afternoon activity following treatment (average activity/minute).
- 4A) Bee fecundity per treatment (number of completed brood cells/treatment).
- 4B) Bee fertility per treatment (number of eggs that hatched/treatment).
- Table 1: number of straws, number of cells, empty nest space (amount of unoccupied space in the nes), nest cap size (size of the cap or covering of the nes), diapause incidence (percentage of organisms that entered diapause), and mortality during pre-diapause, diapause and post-diapause: number of cells (organisms) that died before, during and after diapause, Siblingcide (percentage of organisms that were killed by their siblings)