Data from: Evidence of centromeric histone 3 chaperone involved in DNA damage repair pathway in budding yeast
Data files
Oct 13, 2025 version files 21.36 GB
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Input_MMS_treated-1.fastq.gz
2.90 GB
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Input_MMS_treated-2.fastq.gz
2.94 GB
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Input_MMS_untreated-1.fastq.gz
3.03 GB
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Input_MMS_untreated-2.fastq.gz
3.13 GB
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MMS_treated-1.fastq.gz
1.73 GB
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MMS_treated-2.fastq.gz
1.76 GB
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MMS_untreated-1.fastq.gz
2.89 GB
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MMS_untreated-2.fastq.gz
2.99 GB
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README.md
1.91 KB
Abstract
The centromeric protein-A (CENP-A) is an evolutionary conserved histone H3 variant that marks the identity of the centromeres. Several mechanisms regulate the centromeric deposition of CENP-A as its mislocalization causes erroneous chromosome segregation, leading to aneuploidy-based diseases, including cancers. The most crucial deposition factor is a CENP-A specific chaperone, HJURP (Scm3 in budding yeast), which specifically binds to CENP-A. However, the discovery of HJURP as a DDR (DNA damage repair) protein and evidence of its binding to Holliday junctions in vitro indicate a CENP-A-deposition-independent role of these chaperones. In this study, using budding yeast, we demonstrate that Scm3 is crucial for the DDR pathway as scm3 cells are sensitive to DNA damage. We further observe that the scm3 mutant genetically interacts with the rad52 DDR mutant and is compromised in activating DDR-mediated arrest. We demonstrate that Scm3 associates with the DNA damage sites and undergoes posttranslational modifications upon DNA damage. Overall, from this report and earlier studies on HJURP, we conclude that DDR functions of CENP-A chaperones are conserved across eukaryotes. Thus, the revelation that these chaperones confer genome stability in more than one pathway has clinical significance.
https://doi.org/10.5061/dryad.gb5mkkx1t
Description of the data and file structure
The genome-wide binding of Scm3 was evaluated from MMS-treated or untreated cells using ChIP-seq assay.
Files and variables
File: Input_MMS_treated-1.fastq.gz
Description: Input fasta files from MMS treated sample
File: Input_MMS_treated-2.fastq.gz
Description: Input fasta files from MMS treated sample
File: Input_MMS_untreated-1.fastq.gz
Description: Input fasta files from untreated sample
File: Input_MMS_untreated-2.fastq.gz
Description: Input fasta files from untreated sample
File: MMS_treated-1.fastq.gz
Description: IP fasta files from MMS treated sample
File: MMS_treated-2.fastq.gz
Description: IP fasta files from MMS treated sample
File: MMS_untreated-1.fastq.gz
Description: IP fasta files from untreated sample
File: MMS_untreated-2.fastq.gz
Description: IP fasta files from untreated sample
Code/software
The raw reads from ChIP-seq data were processed using FastQC (Andrews S., 2010) for quality assessment and pre-processing, which includes removing the adapter sequences and low quality bases (<q30) using TrimGalore (Krueger F., 2015). The pre-processed high quality read data was aligned to reference Saccharomyces cerevisiae genome database using Bowtie, an ultrafast, memory-efficient short read aligner geared towards quickly aligning large sets of short DNA sequences (reads) to large genomes. The aligned files (BAM files) were used for peak calling using MACS2 software with an input sample as a control. The bedgraph files generated from MACS2 software were used to plot the graphs using the IGV browser (Robinson et al., 2011).