A bioprinted model of pregnant human uterine myometrium
Data files
Oct 31, 2025 version files 36.97 MB
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Live_Dead_220516LB_3DAnimation.avi
34.56 MB
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README.md
3.72 KB
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TMT_Proteomics_Primary_vs_Parent_Tissue.txt.xlsx
801.33 KB
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TMT_Proteomics_Ring_vs_Parent_Tissue.xlsx
801.16 KB
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TMT_Proteomics_Ring_vs_Primary.txt.xlsx
801.70 KB
Abstract
We have utilized primary human uterine smooth muscle cells to bioprint myometrial tissue rings containing >75% viable cells with elongated, smooth muscle morphology. Immunofluorescence confirmed expression of smooth muscle markers (caldesmon, alpha smooth muscle actin, and smooth muscle myosin), contractile-associated proteins (oxytocin receptor, prostaglandin receptors and connexin-43), and steroid hormone receptors (estrogen and progesterone receptors) characteristic of pregnant human uterine myometrium. Bioprinted tissue ring area contracted in response to physiological agonists oxytocin (p<0.001), prostaglandin F2 alpha (p=0.003), and prostaglandin E2 (p<0.001), and relaxed in response to the nitric oxide donor S-nitrosoglutathione (p=0.0004).
Dataset DOI: 10.5061/dryad.gf1vhhn0m
Description of the data and file structure
Total proteomic profiles (n=3 for each group) of parent myometrial tissue, primary uterine smooth muscle cells grown in 2-dimensional culture, and synthetic myometrial tissues were collected and analyzed via TMT mass tag quantitative proteomics
Files and variables
File: TMT_Proteomics_Primary_vs_Parent_Tissue.txt.xlsx
Description: Tandem Mass Tag Quantitative Proteomic Analysis of Primary Cells vs Parent Tissue
Variables
- Column A (ID)– UNIPROT ID
- Column B (Description)- Protein Description
- Column C (CoveragePerc)- Percentage of protein coverage mapped
- Column D (NumPep)- Number of Peptides Mapped
- Column E (NumPSMSs)- Number of Peptide Scoring Matches Mapped
- Column F (NumUniqPep)- Number of Unique Peptides Mapped
- Column G (imput)- Where Imputed Peptides Used (Yes/NO)
- Column H (numImpute)- Number of Imputed Peptides
- Column I (protein)- UNIPROT ID
- Column J (mean_P)- Mean Expression Level from TMT Analysis of Primary Cells
- Column K (mean_T)- Mean Expression Level from TMT Analysis of Parent Tissue
- Column L (pval)- P-value
- Column M (adjpval)- Adjusted P-value
- Column N (FC_ PvsT)- Fold Change Primary Cells vs Parent Tissue
File: TMT_Proteomics_Ring_vs_Parent_Tissue.xlsx
Description: Tandem Mass Tag Quantitative Proteomic Analysis of Ring vs Parent Tissue
Variables
- Column A (ID)– UNIPROT ID
- Column B (Description)- Protein Description
- Column C (CoveragePerc)- Percentage of protein coverage mapped
- Column D (NumPep)- Number of Peptides Mapped
- Column E (NumPSMSs)- Number of Peptide Scoring Matches Mapped
- Column F (NumUniqPep)- Number of Unique Peptides Mapped
- Column G (imput)- Where Imputed Peptides Used (Yes/NO)
- Column H (numImpute)- Number of Imputed Peptides
- Column I (protein)- UNIPROT ID
- Column J (mean_R)- Mean Expression Level from TMT Analysis of Ring
- Column K (mean_T)- Mean Expression Level from TMT Analysis of Parent Tissue
- Column L (pval)- P-value
- Column M (adjpval)- Adjusted P-value
- Column N (FC_ RvsT)- Fold Change Ring vs Parent Tissue
File: TMT_Proteomics_Ring_vs_Primary.txt.xlsx
Description: Tandem Mass Tag Quantitative Proteomic Analysis of Ring vs Primary Cells
Variables
- Column A (ID)– UNIPROT ID
- Column B (Description)- Protein Description
- Column C (CoveragePerc)- Percentage of protein coverage mapped
- Column D (NumPep)- Number of Peptides Mapped
- Column E (NumPSMSs)- Number of Peptide Scoring Matches Mapped
- Column F (NumUniqPep)- Number of Unique Peptides Mapped
- Column G (imput)- Where Imputed Peptides Used (Yes/NO)
- Column H (numImpute)- Number of Imputed Peptides
- Column I (protein)- UNIPROT ID
- Column J (mean_R)- Mean Expression Level from TMT Analysis of Ring
- Column K (mean_P)- Mean Expression Level from TMT Analysis of Primary Cells
- Column L (pval)- P-value
- Column M (adjpval)- Adjusted P-value
- Column N (FC_ RvsP)- Fold Change Ring vs Primary Cells
File: Live Dead 220516LB 3DAnimation.avi
Description: 3D cross section of human uterine smooth muscle rings stained to identify live cells (Calcein-AM Green) and dead cells (Propidium Iodide Red) with the nuclei labeled in blue (Hoechst).
Variables
Code/software
These are Excel Spreadsheets and .avi movie files that can be opened with any free software that supports this file type.