Data from: Raw qPCR, cAMP ELISA, and proton flux data from marine medaka exposed to CO₂-induced seawater acidification
Data files
May 13, 2026 version files 18.08 KB
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adcy_gill_gene_expression.csv
1.29 KB
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adcy_larva_gene_expression.csv
1.47 KB
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cAMP_contents.csv
1.61 KB
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itr_gill_gene_expression.csv
1.44 KB
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itr_larva_gene_expression.csv
1.50 KB
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MO_gene_expression.csv
1.68 KB
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Proton_secretion.csv
4.64 KB
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README.md
4.45 KB
Abstract
This dataset contains the raw data associated with the manuscript “Isotocin receptor-adenylyl cyclase signaling mediates pH compensation in marine fish exposed to CO₂-induced acidification.” It includes quantitative PCR (qPCR) data for genes involved in acid-base regulation, cAMP measurements determined by ELISA, and proton secretion data obtained from yolk-sac ionocytes. The dataset also contains results from Morpholino (MO)-mediated knockdown experiments targeting ITRb and ADCY5, used to assess their functional roles in epithelial H⁺ secretion under hypercapnic conditions. Data were collected from marine medaka (Oryzias melastigma) exposed to control (pH 8.1, pCO2~~0.03 kPa) and CO2~-acidified seawater (pH 7.6, pCO~2~~0.14 kPa), supporting analyses of hormonal regulation and the molecular mechanisms underlying acid-base regulation during ocean acidification.
Dataset DOI: 10.5061/dryad.h44j0zq00
Description of the data and file structure
This dataset provides raw experimental data, including qPCR, cAMP measurements, proton secretion, and Morpholino (MO) knockdown results, from adult gills and larvae of marine medaka (Oryzias melastigma) exposed to control and CO2-acidified seawater.
Files and variables
File: adcy_gill_gene_expression.csv
Description: Relative gene expression of adenylyl cyclase in adult gill exposed to control and acidified seawater.
Variables
- Gene_name: Name of the gene analyzed, including the two isoforms of adenylyl cyclase (adcy).
- Group: Control (pH 8.1) and CO2-treatment (pH 7.6).
- Transfer_time_hours: Duration (in hours) that adult medaka were exposed to the acidified condition following transfer.
- Gene_expression: Relative gene expression level normalized to the housekeeping gene rpl7.
File: adcy_larva_gene_expression.csv
Description: Relative gene expression of adenylyl cyclase in 5 dpf embryos and newly hatched larvae exposed to control and CO2-acidified seawater.
Variables
- Gene_name: Name of the gene analyzed, including the two isoforms of adenylyl cyclase (adcy).
- Group: Control (pH 8.1) and CO2-treatment (pH 7.6).
- Stage: Developmental stage of medaka, including 5 days post-fertilization (5 dpf) and hatching.
- Gene_expression: Relative gene expression level normalized to the housekeeping gene rpl7.
File: cAMP_contents.csv
Description: cAMP levels measured in 5 dpf embryos following MO-mediated knockdown experiments.
Variables
- Treatment: Control (pH 8.1) and CO2-treatment (pH 7.6).
- Group: Experimental treatment groups, including WT (wild type), ITRb_MO (morpholino knockdown of itrb), ADCY5_MO (morpholino knockdown of adcy5), and ITRb+ADCY5_MO (morpholino double knockdown of itrb and adcy5).
- cAMP contents (pmol/ embryo): Intracellular cyclic AMP concentration, expressed as picomoles (pmol) per embryo.
File: itr_gill_gene_expression.csv
Description: Relative gene expression of the isotocin receptor in adult gills exposed to control and CO2-acidified seawater.
Variables
- Gene_name: Name of the gene analyzed, including the two isoforms of isotocin receptor (itr).
- Group: Control (pH 8.1) and CO2-treatment (pH 7.6).
- Transfer_time_hours: Duration (in hours) that adult medaka were exposed to the acidified condition following transfer.
- Gene_expression: Relative gene expression level normalized to the housekeeping gene rpl7.
File: itr_larva_gene_expression.csv
Description: Relative gene expression of the isotocin receptor in 5 dpf embryos and newly hatched larvae exposed to control and CO2-acidified seawater.
Variables
- Gene_name: Name of the gene analyzed, including the two isoforms of isotocin receptor (itr).
- Group: Control (pH 8.1) and CO2-treatment (pH 7.6).
- Stage: Developmental stage of medaka, including 5 days post-fertilization (5 dpf) and hatching.
- Gene_expression: Relative gene expression level normalized to the housekeeping gene rpl7.
File: Proton_secretion.csv
Description: Proton secretion measurements in 5 dpf embryos following Morpholino (MO)-mediated knockdown, obtained using the Scanning Ion-Selective Electrode Technique (SIET).
Variables
- Treatment: Control (pH 8.1) and CO2-treatment (pH 7.6).
- Group: Experimental treatment groups, including WT (wild type), Control_MO (control morpholino), ITRb_MO (morpholino knockdown of itrb), ADCY5_MO (morpholino knockdown of adcy5), and ITRb+ADCY5_MO (morpholino double knockdown of itrb and adcy5).
- [ H] M: Proton concentration, expressed in micromolar (µM).
File: MO_gene_expression.csv
Description: Relative expression of acid-base-related genes in 5 dpf embryos after MO-mediated knockdown experiments.
Variables
- Gene_name: The gene of acid-base-related
- Treatment: CO2-treatment (pH 7.6).
- Stage: 5 days post-fertilization (5 dpf)
- Group: Experimental treatment groups, including WT (wild type) and ITRb+ADCY5_MO (morpholino double knockdown of itrb and adcy5).
- gene_expression: Relative gene expression level normalized to the housekeeping gene rpl7.