Data from: Exploring thermal tolerance across time and space in a tropical bivalve, Pinctada margaritifera
Data files
Mar 31, 2025 version files 328.28 KB
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e1-binary.xlsx
276.82 KB
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e1-binomial.xlsx
14.31 KB
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e2.xlsx
11.36 KB
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e3.xlsx
21.97 KB
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README.md
3.82 KB
Abstract
Ectotherm vulnerability to climate change is predicted to increase with temperature variation. Still, translating laboratory observations of organisms’ heat-stress responses to the natural fluctuating environment remains challenging. In this study, we used an integrative framework combining insights from thermal death time (TDT) curves and physiological reaction norms to precisely capture Pinctada margaritifera’s thermal performance and tolerance landscape. We then applied this integrative model to predict individuals’ cumulative heat injury as a function of actual temperature conditions documented at five contrasting islands across French Polynesia. Substantial injury was predicted for spats (ranging from 30.24% to 29.62%) when exposed to eight consecutive extreme low tide events in Nuku Hiva. Overall, this study highlights the potential of this framework to effectively quantify the impact of extreme events, such as marine heatwaves, and to guide resource management initiatives.
Description of the data and file structure
Results from E1 experiment
#e1-binary
Column names indication:
tank : Tank ID
replicate : vial replicate ID
time : time of exposure at the thermal condition (in minutes)
target_temp : targeted temperature condition in the tank (in degree Celsius)
mean_temp : mean measured temperature in the tank over the entire experiment duration (in degree Celsius)
sd_temp : standard deviation of the measured temperaturein the tank over the entire experiment duration (in degree Celsius)
status : status of each larva success, alive(1) / dead (0)
#e1-binomial
success : number of alive larva in the vial at Tfinal (k)
tot : total number of embryo in the vial at Tinitial (n)
dead : number of dead larva in the vial (n-k)
surv : percentage of survival probability (k/n)
Results from E2 experiment
#e2
Specific colnames indications
sp : scientific name of the studied species
phylum : phylum level of classification of the studied species
class : class level of classification of the studied species
ID : life stage identification
date : date of the experiment run
age : age of the individuals (expressed in months)
mean_FW(g) : mean fresh weight of the individuals
sd_FW(g) : standard deviation of the fresh weight of the individuals
lifestage : lifestage feature of the individuals
replicate : ID of the replicated run
target_temp : target temperature of exposure of the experimental tank (expressed in degree Celsius)
mean_temp: mean temperature of exposure recorded in the experimental tank (expressed in degree Celsius)
sd_temp : standard deviation the temperature of exposure recorded in the experimental tank (expressed in degree Celsius)
adj_temp : adjusted temperature of exposure recorded in the experimental tank (round the value to the nearest whole number; expressed in degree Celsius)
LT50_min : time at which mortality in the tank reached 50 % (in minutes)
LOG_LT50 : log10 transformed LT50_min
Results from E3 experiment
#e3
Specific colnames indications:
measure : physiological rate measured (respiration; ingestion, or scope for growth)
date : the date on which the measurement was taken
replicate : ID of the replicated run
tank : ID of the experimental tank
target_temp : target temperature of exposure of the experimental tank (expressed in degree Celsius)
mean_temp: mean temperature of exposure recorded in the experimental tank (expressed in degree Celsius)
sd_temp: standard deviation the temperature of exposure recorded in the experimental tank (expressed in degree Celsius)
adj_temp: adjusted temperature of exposure recorded in the experimental tank (round the value to the nearest whole number; expressed in degree Celsius)
chamber : ID microchamber used for physiological measurements
ind : ID of the oyster
FW_tot : Total fresh weight of the measured oyster (in grams)
FW_flesh : Total fresh weight of the flesh of the measured oyster (in grams)
DW_flesh : Total dry weight of the flesh of the measured oyster (in grams)
raw_rate : Raw measured physiological rate (in mgO2/h/DW - for respiration; cell/h/DW - for ingestion; and J/h/DW - for scope for growth)
Ys : raw-rate corrected with the allometric coefficient
rate_norm : normalized Ys, by the control condition (i.e. 28°C)
Codes
A R code is available to reproduce analyses (3.1. Estimating thermal thresholds) done in the study.
All data analyses and modelling were done using R version 4.3.1 (R Core Team, 2023).
Thresholds of statistical significance (α) were set at 0.05, unless specifically stated.
Three different experiments (E1, E2 and E3, details below) were performed using hatchery-produced individuals issued from two reproduction events. The E1 experiment aimed to assess larval development completion (i.e., performance and upper limits) under a wide range of temperatures and intensities (i.e. time of exposure).In turn, the E2 and E3 allowed investigating the thermal sensitivity of spats experiencing stressful and permissive temperatures, respectively.