Seasonal expansion of vector mosquito communities beyond village boundaries along an agropastoral transect and implications for disease transmission in Southwestern Mali
Data files
Oct 16, 2026 version files 119.34 KB
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BGS_Transect_241220_Update.xlsx
108.94 KB
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README.md
10.39 KB
Abstract
Biogents Sentinel Traps with Heat (BGSH) were deployed along a 2.5-km transect extending from the village of Sogolombougou, Mali, into surrounding agropastoral habitats. Twenty-four traps positioned at six distances (0-2500 m) were operated for four consecutive nights during four seasonal phases (dry, dry-to-rainy, rainy, and rainy-to-dry) over two consecutive years. Captured mosquitoes were identified morphologically and members of the Anopheles gambiae complex were characterised by PCR. Female anophelines were screened for Plasmodium falciparum sporozoites using CSP-ELISA.
Dataset DOI: 10.5061/dryad.j0zpc86zh
Description of the data and file structure
Dataset description
This dataset contains entomological surveillance data collected along an agropastoral transect in southwestern Mali to investigate seasonal variation in mosquito abundance, community composition, and spatial distribution at increasing distances from human settlements.
The dataset includes sampling dates, seasonal periods, sampling locations, trap identifiers, distances from human habitations, mosquito species counts, sex-specific abundance, and molecular identification or pathogen-detection results.
Each row represents a sampling record associated with a particular trap, location, and collection period.
2. Data format
The data are provided in Microsoft Excel format (.xlsx).
Files can be opened using Microsoft Excel or LibreOffice Calc. Statistical analyses were conducted using JMP version 16 (SAS Institute Inc.).
| Abbreviation | Definition |
|---|---|
| R | Range: sampling position along the transect |
| BG | Biogents Sentinel trap |
| An. | Anopheles |
| Ae. | Aedes |
| Cx. | Culex |
| An. gambiae s.l. | Anopheles gambiae sensu lato |
| s.l. | Sensu lato; species complex in the broad taxonomic sense |
| s.s. | Sensu stricto; species in the strict taxonomic sense |
| Uf | Unfed female mosquito |
| NA | Not applicable |
| PCR | Polymerase chain reaction |
| CSP | Circumsporozoite protein, where applicable |
| spp. | Multiple species within a genus |
Files and variables
File: BGS_Transect_241220_Update.xlsx
Description:
The data are provided in Microsoft Excel format (.xlsx).
Files can be opened using Microsoft Excel or LibreOffice Calc. Statistical analyses were conducted using JMP version 16 (SAS Institute Inc.).
Sampling and environmental variables
| Column name | Description | Unit or coding |
|---|---|---|
| Date | Date of mosquito collection | DD.MM.YYYY |
| Period | Seasonal collection period | Categorical: dry, rainy, dry-to-rainy, or rainy-to-dry transition, as recorded |
| Period by year | Seasonal period associated with the sampling year | Categorical |
| Jour | Sampling day within the collection period | Jour 1 = Day 1, etc. |
| Position | Sampling range or position along the transect | Range1, Range2, etc. |
| Trap.Code | Unique identification code assigned to each trap | R1BG1 = Range 1, Biogents trap 1 |
| Distance des habitations humaines (m) | Distance between the sampling location and human habitations | Metres (m) |
| Heatpatch & lure usage | Record of heat source and attractant use with the trap | Recorded status; NA = not applicable |
| Start.time | Start of the mosquito collection interval | Local time, 24-hour clock |
| End.time | End of the mosquito collection interval | Local time, 24-hour clock |
Example: R3BG2 identifies Biogents Sentinel trap number 2 positioned at sampling Range 3.
5. Mosquito abundance and sex-specific variables
All mosquito abundance variables represent counts of individual mosquitoes, unless otherwise specified.
| Column name or variable group | Description |
|---|---|
| Total An.gambiae s.l. | Total number of Anopheles gambiae sensu lato collected |
| Total An.gambiae s.l. Female | Number of female An. gambiae s.l. collected |
| Total An.gambiae s.l. Male | Number of male An. gambiae s.l. collected |
| An.gsl.uf | Number of unfed female An. gambiae s.l. |
| An.gsl.bf | Female An. gambiae s.l. classified as blood-fed |
| An.gsl.gra | Female An. gambiae s.l. classified as gravid |
| An.gsl.halfgra | Female An. gambiae s.l. classified as half-gravid |
| Total An.coustani | Total number of Anopheles coustani collected |
| An.coustani Female | Number of female An. coustani collected |
| An.coustani Male | Number of male An. coustani collected |
| Total An. pharoensis | Total number of Anopheles pharoensis collected |
| An.pharoensis Female | Number of female An. pharoensis collected |
| An.pharoensis Male | Number of male An. pharoensis collected |
| Total Ae.aegypti | Total number of Aedes aegypti collected |
| Ae.aegypti Female | Number of female Ae. aegypti collected |
| Ae.aegypti Male | Number of male Ae. aegypti collected |
| Total Cx.quinquefasciatus | Total number of Culex quinquefasciatus collected |
| Cx.quinquefasciatus Female | Number of female Cx. quinquefasciatus collected |
| Cx.quinquefasciatus Male | Number of male Cx. quinquefasciatus collected |
| Man.SPP | Count recorded under the original Man.SPP taxonomic category; exact taxonomic designation to be confirmed |
| Toxorhynchite | Count of mosquitoes assigned to the Toxorhynchites category |
6. Molecular identification and pathogen-detection variables
| Column name | Description |
|---|---|
| PCR.An.col | PCR identification results for Anopheles coluzzii |
| PCR.An.gam | PCR identification results for Anopheles gambiae sensu stricto |
| PCR.An.ara | PCR identification results for Anopheles arabiensis |
| PCR.H.col.gam | PCR results recorded for the coluzzii/gambiae hybrid category |
| PCR.H.ara.col | PCR results recorded for the arabiensis/coluzzii hybrid category |
| PCR.H.ara.gam | PCR results recorded for the arabiensis/gambiae hybrid category |
| PCR.An.spp | PCR results assigned to an unresolved Anopheles species category |
| P. Falciparum | Recorded Plasmodium falciparum detection results |
These columns appear to contain numerical counts. Their exact interpretation should be verified against the laboratory records, particularly whether zero represents a negative test, an absence of identified specimens, or no specimens tested.
Code/software
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Numerical mosquito abundance values represent counts of individuals.
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Zero (0) in an abundance column indicates that no mosquitoes were recorded in that category for the corresponding sampling record.
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NA indicates not applicable, rather than a numerical zero.
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Distances are expressed in metres.
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Sampling times are expressed using the 24-hour clock.
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Taxonomic abbreviations follow standard entomological nomenclature.
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Data analysis and reproducibility
The dataset was analysed using JMP version 16 (SAS Institute Inc.). The Excel files contain the tabulated data used for the entomological analyses presented in the associated manuscript.
No analytical scripts are included with this submission.