CO2 response curves and leaf reflectance data for La Selva Biological Station, Costa Rica
Data files
Jul 07, 2026 version files 1.57 MB
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README.md
2.30 KB
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RodriguezCaton_2024_Aci_data.csv
103.14 KB
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RodriguezCaton_2024_Reflectance.csv
1.46 MB
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RodriguezCaton_2024_SampleDetails.csv
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Abstract
This dataset includes data collected in a broadleaf wet tropical forest located La Selva Biological Station in the Costa Rican Caribbean region (10.432S, -84.0106W). The site is an old-growth forest equipped with an Eddy Covariance Tower. The dataset contains leaf-level gas exchange and hyperspectral reflectance data collected between May 2023 and February 2024 at La Selva.
Dataset DOI: 10.5061/dryad.j6q573npr
Description of the data and file structure
CO2 response curves and leaf reflectance at La Selva Biological Station, Costa Rica
https://doi.org/10.5061/dryad.j6q573npr
Description of the data and file structure
This folder contains THREE files:
FILE #1- "RodriguezCaton_2024_SampleDetails.csv"
This file has 7 columns:
SampleID: The SampleID corresponds to the metadata associated with each measurement: date, replicate, species code, leaf number, light/dark adapted.
For example the code "20230513.1.VISE.1.D" is composed by:
'20230513': Date. Year, month and day in the format YYYYMMDD
'4': Number of replicate of the day or 'hour' (note this is not the actual hour of the day). In this example '4' corresponds to the fourth replicate of the day for this species
'VISE': Species code
'1': Leaf number. For each replicate 3 leaves were sampled.
Date: Year, month, and day in the format YYYYMMDD
Site: corresponds to the site code, in this study, LSVA refers to the site La Selva, Costa Rica
Species_Name: Scientific Name of the species
Species_Code: The code used in this study to identify each species
Light_or_Dark_adapted: Specifies whether the leaf was adapted to Light = 'L' or Dark = 'D'
FILE #2- "RodriguezCaton_2024_Aci_data.csv"
This file contains the A/Ci response curves and has 11 columns:
SampleID: Same as FILE#1
obs: Observation number
A: μmol m-2 s- Assimilation rate
Ci: μmol mol-1 Intercellular CO2
CO2_s: μmol mol-1 Sample cell CO2 concentration
CO2_r: μmol mol-1 Reference cell CO2 concentration
gsw: mol m-2 s-1 Stomatal conductance to water vapor
Pa: kPa Atmospheric pressure
Qin: μmol m-2 s-1 PPFD incident on the leaf
RHcham: % Relative humidity in the chamber
Tleaf: °C Leaf temperature
FILE #3- "RodriguezCaton_2024_Reflectance.csv"
This file contains the leaf reflectance data and has 2152 columns:
Column 1 "SampleID" Same as FILE#1
Column 2 to 2152 reflectance measurements from 350 nm to 2500 nm (1 nm resolution).
Photosynthetic carbon dioxide (CO2) response curves and hyperspectral reflectance were measured in sun-exposed leaves from six tree species, including five overstory species (Pentaclethra macroloba, Virola koschnyi, Sacoglottis trichogyna, Goethalsia meiantha, Virola sebifera) and one understory species (Warszewiczia coccinea). Most leaves were light adapted but the dataset includes some dark adapted leaves. 20-50cm branches were detached from the main tree and recut underwater to avoid xylem cavitation and maintain the water column intact. We selected a healthy and mature leaf from each branch to run the CO2 response curve. CO2 response curves were performed for each leaf using the LI-6800 portable photosynthesis system (LICOR, Lincoln, NE, USA). For each CO2 response curve, CO2 concentration varied from 50 to 1400 μmol/mol with a reference CO2 of 420 μmol/mol. Leaves were inducted with 1000 or 1500 μmol/mol irradiances, 30° C temperature, and 65 % relative humidity. Observations were initiated when carbon assimilation rate (A) was stable, with values no less than 5 μmol/mol and stomatal conductance values greater than or equal to 0.06 mol H2O m-2 s-1, preferably. After performing the CO2 response curves for each leaf, we measured leaf reflectance using a hyperspectral spectroradiometer (SVC, HR-1024i, Spectra Vista Corporation, Poughkeepsie, NY, USA) equipped with a leaf clip LC-RP-PRO. The specifications on this spectroradiometer were a 25° FOV optic fiber with an autointegration time of over 3-5 seconds to maximize signal. We run R packages spectrolab and pavo to splice sensor overlap regions and obtain 1 nanometer (nm) resolution reflectance between 350 and 2500 nm, respectively. A white reference panel attached to the leaf clip was used before each reflectance measurement. We conducted three spectral measurements per leaf and the values reported here correspond to leaf averages. Spectral measurements were performed on intact tissue on the adaxial part of the leaves.