Hydrothermal modification of porous silica microbands improves single-cell assays of ether-lipid metabolism
Data files
Jun 22, 2026 version files 224.13 KB
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Figure_2E.csv
162 B
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Figure_3B.csv
207.47 KB
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Figure_3C.csv
5.14 KB
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Figure_3D.csv
935 B
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Figure_5D.csv
7.25 KB
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Figure_5E.csv
1.46 KB
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README.md
1.72 KB
Abstract
The ability to separate and detect lipid metabolites in single cells has been achieved using a miniaturized thin-layer chromatography (TLC) platform termed picoTLC (pTLC). However, continued innovations in matrix fabrication, lipid reporter development, and single-cell workflow will broaden the applicability of pTLC to single-cell assays. In this study, we report three advances: a simple post-fabrication process [hydrothermal treatment (HT)] to modify the pTLC silica matrix, a novel clickable lipid reporter to assay metabolism of alkylglycerol (an ether lipid), and a fix-click strategy to prepare cells for pTLC assay of the metabolized lipid reporter. These combined advances enabled the identification of metabolites formed from the novel lipid reporters in single leukemic cells and will further broaden the application of pTLC to report lipid metabolism at the single-cell level. Tabulated raw data used in Figure 2E, Figure 3B, Figure 3C, Figure 3D, Figure 5D, and Figure 5E are available on Dryad. These data are in CSV file version and can be opened by can be opened by Microsoft Excel.
Dataset DOI: 10.5061/dryad.jm63xsjr9
Description of the data and file structure
- Figure_2E.csv. Tabulated raw data of the CSV file version. It shows the measured fluorescence intensity of porous silica microbands after conjugation with FITC for pTLC with or without HT treatment. 4 chips per condition. The file can be opened by Microsoft Excel.
- Figure_3B.csv. Tabulated raw data of the CSV file version. It shows the measured fluorescence intensity at green and red channels along the distance of the stationary phase for conventional TLC, pTLC without HT treatment, and pTLC after HT treatment. The file can be opened by Microsoft Excel.
- Figure_3C.csv. Tabulated raw data of the CSV file version. It shows the calculation of resolutions of neighboring peaks (R12, R23, R34, R45) on conventional TLC, pTLC without HT treatment, and pTLC after HT treatment. 4 chips per condition. The file can be opened by Microsoft Excel.
- Figure_3D.csv. Tabulated raw data of the CSV file version. It shows the calculation of retention factors (Rf) on pTLC without HT treatment and pTLC after HT treatment. 4 chips per condition. The file can be opened by Microsoft Excel.
- Figure_5D.csv. Tabulated raw data of the CSV file version. It shows the percentage of lipids (reporter-1, M1, and M2) of 50 single cells analyzed by pTLC. The file can be opened by Microsoft Excel.
- Figure_5E.csv. Tabulated raw data of the CSV file version. It shows the percentage of lipids (reporter-2, M1, and M2) of 50 single cells analyzed by pTLC. The file can be opened by Microsoft Excel.