During the fungal exploration from the Himalayan moist temperate forests of Pakistan, different fruiting bodies of the genus Pseudosperma were collected that resulting in one new species, i.e., Pseudosperma khanspuricum, and one new record, Pseudosperma aureocitrinum. Pseudosperma khanspuricum is characterized by its campanulate, fibrillose, smaller (up to 2.2 cm), brownish yellow pileus with acute umbo, larger (up to 5.2 cm) stipe, creamy white to light yellow lamellae, and smaller (8.9 × 5.8 µm) basidiospores. The second species Pseudosperma aureocitrinum has been reported for the first time from Pakistan. Molecular phylogenetic evaluation deduced from the nrDNA internal transcribed spacer region (ITS) and larger subunit region (LSU) validates this as a new species and a new record, with this addition species of Psudosperma count to be 13 from Pakistan.