Dataset DOI: 10.5061/dryad.jsxksn0pr

Description of the data and file structure

This dataset contains the data required to replicate quantification and analyses in Starost et al.:

Receptor tyrosine kinase (RTK) signaling drives cancer and is a validated therapeutic target. Modulators of RTK signaling can reveal mechanisms of oncogenesis and offer new therapeutic targets. Golgi phosphoprotein 3 (GOLPH3) is a Golgi-localized oncoprotein that promotes signaling downstream of mTOR. Here, examination of RTK signaling indicated that GOLPH3 acted at the level of the RTK and increased all downstream signaling. We found that GOLPH3 enhanced the delivery of RTKs to the plasma membrane. This role was shared with its binding partner Myosin 18A (MYO18A) and depended on the interaction of GOLPH3 with MYO18A. The GOLPH3-MYO18A complex at the Golgi apparatus was required and rate-limiting for RTK signaling across the cell types and receptors assessed. Our findings provide insight into the relationship between the function of GOLPH3 at the Golgi and its role as a cancer driver, highlighting its potential as a therapeutic target in cancer.

All data are separated by Figure as outlined in the manuscript. Each Figure is subdivided into folders/directories representing each figure panel. Each contains raw data files and quantification as indicated in the manuscript (and this README), and any extra information needed to re-analyze the data.

Files and variables

Each Figure Folder (e.g., Figure 1) contains sub-folders for each individual panel in the manuscript (A, B, C, etc.). Within each panel folder are sub-folders containing experimental or biological replicates that contain one or more of the following:

• Western blot raw 16-bit TIFFs which can be analyzed by the free FIJI/ImageJ software. These are accompanied by an Excel spreadsheet containing densitometry values and statistical analyses obtained for each treatment in each image. The images include labeling (e.g., date and/or experiment number and antibody used for blotting) corresponding to labeling within the associated Excel spreadsheet. The Excel spreadsheet provides annotation indicating the order of lanes for each set of blots, measured integrated intensity, background intensity values, and calculation of relative intensities with statistical analyses.
• TIFF format immunofluorescence images that can be analyzed by the free FIJI/ImageJ software along with an Excel spreadsheet with measured values (as indicated by labeling within the Excel spreadsheet) and statistical analyses obtained for each treatment.
• Additional Excel spreadsheets with raw output values generated from additional experiments, as described in the spreadsheets.
• All Excel spreadsheets contain labeling in rows, columns, and tabs to enable interpretation of the data and the analyses that are provided. This README (and the published manuscript) provide additional helpful context.

File: Figure_1.zip

Description: Both GOLPH3 and MYO18A are required for EGFR signaling at the level of the receptor, independently of mTOR. (A) A549 cells were transfected with the indicated siRNAs (GOLPH3 - siG3, MYO18A - siM18A), serum starved, pre-treated (as indicated) with wortmannin (100 nM for 10 minutes), stimulated (or not) with EGF (100 ng/mL for 10 minutes), and analyzed by Western blotting (B) Quantification of relative intensity from (A) of indicated phosphoprotein (EGFR or AKT) normalized to total protein (EGFR or AKT, respectively), or phospho-ERK normalized to GAPDH. (C) HeLa cells were transfected with indicated siRNAs, serum starved, stimulated (or not) with EGF (100 ng/mL for 5 minutes), analyzed by Western blotting using the indicated antibodies. (D) Quantification of (C) of the indicated phosphoprotein (EGFR or AKT) normalized to total protein (EGFR or AKT, respectively) or phospho-ERK normalized to GAPDH. (E) HeLa cells were treated with EGF (100 ng/mL for 5 minutes) with or without pre-treatment with wortmannin (wort) (100 nM for 10 minutes) and analyzed by Western blotting using the indicated antibodies. (F) Quantification of (E) for phospho-EGFR normalized to total EGFR and phospho-AKT relative to total AKT. Graphs in excel sheets indicate means and 95% confidence intervals for pooled data from multiple biological replicates, indicated as red dots. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 determined by two-sided t-test with Holm-Bonferroni correction for multiple comparisons. p-values for (D) are calculated using a one sample t-test (compared to the normalized control value, which was set at 1) with Holm-Bonferroni correction for multiple comparisons.

Data files contain western blot images and Excel spreadsheets with quantification/statistical analysis. File names of Western blot TIFF images indicate the specific antibody used to probe the membrane. Annotation within the images indicate which replicate experiment corresponds to each blot, and allows association with the data in the Excel spreadsheet. p - indicates phosphorylated protein. Tot - indicates Total protein.

File: Figure_2.zip

Description: Multiple growth factor receptors require GOLPH3 and MYO18A for proper signaling. (A) HeLa cells were transfected with the indicated siRNAs, serum starved, pretreated (as indicated) with wortmannin (100 nM for 10 minutes), stimulated (or not) with insulin (10 μg/ml for 5 minutes), and analyzed by Western blotting using the indicated antibodies. (B) Quantification from (A) of phospho-AKT normalized to total AKT or phospho-ERK normalized to GAPDH. (C) HeLa cells were transfected with the indicated siRNAs and plasmids, serum starved, stimulated (or not) with PDGF-BB (50 ng/mL for 5 minutes), and analyzed by Western blotting with the indicated antibodies. (D) Quantification of phosphorylation of indicated proteins in (C) normalized to total protein. (E) HeLa cells were transfected with the indicated siRNAs and plasmids, serum starved, stimulated (or not) with PDGF-BB (50 ng/mL for 5 minutes), and analyzed by Western blotting with the indicated antibodies.  (F) Quantification of (E) for phospho-PDGFRb (Tyr⁷⁵¹) relative to total PDGFRb and phospho-AKT (Ser⁴⁷³) relative to total AKT. Means and 95% confidence intervals are indicated for pooled data, with biological replicates indicated by red dots. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 determined by two-sided t-test with Holm-Bonferroni correction for multiple comparisons. p-values for (B) and (D) are calculated using a one sample t-test (compared to the normalized control value, which was set at 1) with Holm-Bonferroni correction.

These files contain western blot images and excel sheets with quantification/statistical analysis similar to Figure_1. File names indicate the specific antibody used to probe the membrane. p - indicates phosphorylated protein. Tot - indicates Total protein.

File: Figure_3.zip

Description: GOLPH3 and MYO18A are required for growth factor signaling-induced proliferation. (A) HeLa cells were serum starved to induce cell cycle arrest, treated with EGF for 20 hours, and analyzed for relative DNA content. (B) Quantification of HeLa cells in G2/M phase (4N DNA content) after being transfected with indicated siRNAs, serum starved to induce cell cycle arrest, and stimulated with EGF for 16-24 hours, as indicated. (C and D) Quantification of cell proliferation over 5 days (120 hours) for MDA-MB-231 cells treated with siRNA to GOLPH3 or MYO18A (C), with parallel Western blots (D) to validate knockdowns. (E and F) Quantification of cell proliferation over 5 days (120 hours) for immortalized primary human embryonic kidney HEK-TE cells treated with siRNA to GOLPH3 or MYO18A (E), with parallel Western blots (F) to validate knockdowns. Graphs indicate means and 95% confidence intervals from four (for B) or three (for C and E) biological replicates per group. ***p<0.001, ****p<0.0001 compared to control siRNA by two-way ANOVA with Tukey’s post-hoc correction for multiple comparisons.

The spreadsheets/GraphPad contain raw/normalized outputs from experiments.

Figure 3C and Figure 3E nuclei TIFF images are images of cell proliferation assays that were performed on 12-well plates in triplicate using 1 x 10⁴ (MDA-MB-231) or 7 x 10³ (HEK-TE) cells per well. Media was changed 24 hrs after transfection and two days afterward. Cells were fixed in 3.7% paraformaldehyde in PBS at the designated time points (24, 72, and 120 hrs) after siRNA transfection. At the conclusion of the assay, all plates were stained with DAPI, at least 16 (4x4 montage) randomly selected non-overlapping fields were imaged by confocal microscopy and total cell count was determined using ImageJ/FIJI and Cell Profiler Software.

Naming convention for the individual immunofluorescence files includes Cell type (HEKTE or MDA-MB-231), day of growth (Day 1, 3, or 5), siRNA treatment (GOLPH3, MYO18A, or Medium GC negative control), and Replicate#. Other information is extraneous.

Ex. KAS HEKTE grow day1 g3-1_2025-10-27_12.09.28_F00 represents HEKTE cell growth on day 1 with GOLPH3 siRNA for replicate #1

Med is Medium GC negative control

G3 is GOLPH3

M1 is MYO18A siRNA# 1

M3 is MYO18A siRNA# 3

File: Figure_4.zip

Description: GOLPH3 and MYO18A are required for Golgi-to-plasma membrane trafficking of RTKs. (A) Representative immunofluorescence confocal microscopy images of serum starved HeLa cells transfected with indicated siRNAs and plasmids and/or treated with the Golgi poisons brefeldin A (BFA) or golgicide A (GCA). Cells were fixed, left unpermeabilized, and stained for the extracellular domain of the PDGFRb receptor. (B) Quantification of (A) indicating detection of PDGFRb receptor at the plasma membrane relative to total cellular GFP (PDGFRb-GFP) expression. Mean and 95% confidence intervals are graphed for data pooled from three independent experiments, with the number of measured cells (n) indicated. ****p<0.0001 determined by two-sided t-test with Holm-Bonferroni correction for multiple comparisons.

Folders contain experimental replicates. Immunofluorescence tiffs are labelled via well. Ex. W1 is Well 1, W2 is Well 2, etc. A separate Excel spreadsheet in each folder is provided to describe the treatments performed in each well. An additional Excel spreadsheet provides quantification of the indicated measurements for each cell along with calculations and statistical analyses, as indicated by labeling within the file.

File: Figure_5.zip

Description: Overexpression of GOLPH3 or MYO18A enhances growth factor signaling. (A) HeLa cells were transfected with expression vectors for GFP, GOLPH3-IRES-GFP (using an internal ribosome entry site to express GOLPH3 and GFP as separate proteins), or GFP-GOLPH3 (with GFP fused to the N-terminus of GOLPH3). Cells were serum starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes) and analyzed by Western blotting using the indicated antibodies. (B) Quantification of (A) for phospho-EGFR (Tyr¹⁰⁶⁸) relative to total EGFR expression. (C) HeLa cells were transfected with expression vectors for GFP or GFP-MYO18A, serum starved, stimulated (or not) with EGF (100 ng/mL for 5 minutes), and analyzed by Western blotting using the indicated antibodies. (D) Quantification of (C) for phospho-EGFR (Tyr¹⁰⁶⁸) relative to total EGFR expression. Means and 95% confidence intervals are indicated for pooled data from multiple experiments, with individual biological replicates indicated by red dots. **p<0.01, ***p<0.001 by two-sided t-test with Holm-Bonferroni correction for multiple comparisons. In (D), GFP-MYO18A/unstimulated is presented to provide context to the other values, is not included in statistical analyses, with n=2 biological replicates as indicated by red dots.

Files contain western blot raw images as well as quantification/statistical analysis of densitometry similar to Figure_1 and Figure_2.

File: Figure_6.zip

Description: Overexpression of GOLPH3 or MYO18A enhances delivery of RTKs to the PM. (A) Representative immunofluorescence confocal microscopy images of HeLa cells co-transfected with expression vectors for either GFP or PDGFRb-GFP and empty vector or GOLPH3. Cells were serum starved, fixed but not permeabilized, and subjected to immunofluorescence analysis with an antibody to the extracellular domain of PDGFRb to detect surface PDGFRb-GFP. Cells were then re-fixed, permeabilized, and subjected to immunofluorescence analysis to detect GOLPH3. Transfected cells were stratified according to normal or elevated levels of GOLPH3. (B) Quantification of (A) for cell surface PDGFRb-GFP relative to overall expression as assessed by GFP fluorescence. (C) Quantification of GOLPH3. (D) Representative immunofluorescence confocal microscopy images of HeLa cells co-transfected with expression vectors for either GFP or PDGFRb-GFP and empty vector or MYO18A-FLAG. Immunofluorescence was performed as in (A). (E) Quantification of (D) for cell surface PDGFRb-GFP relative to overall expression as assessed by GFP fluorescence. (F) Western blotting performed in parallel to assess expression of MYO18A-FLAG and PGFRb-GFP. Means and 95% confidence intervals are indicated for data pooled from three independent experiments, with number of cells (n) as indicated. ***p<0.001 by two-sided t-test.

Folders contain experimental replicates similar to Figure_4. Immunofluorescence tiffs are labelled via well. Ex. W1 is Well 1, W2 is Well 2, etc. A separate Excel spreadsheet in each folder is provided to describe the treatments performed in each well. An additional Excel spreadsheet provides quantification of the indicated measurements for each cell along with calculations and statistical analyses, as indicated by labeling within the file.

Figure 6-A-B-C HeLa PDGFER-G OE G3 is a folder for HeLa cells that overexpress GOLPH3.

Figure 6-D-E-F HeLa PDGFER-G OE M18A is a folder for HeLa cells that overexpress MYO18A.

File: Figure_7.zip

Description: GOLPH3-driven RTK trafficking depends on interactions with PI4P and MYO18A. (A) Representative immunofluorescence confocal microscopy images of HeLa cells co-transfected with expression vectors for either GFP or PDGFRb-GFP and empty vector, GOLPH3 (WT), MYC-GOLPH3 (N-terminally-tagged to prevent interaction with MYO18A), or GOLPH3-R90L (which does not interact with PI4P). Cells were serum starved, fixed but not permeabilized, and subjected to immunofluorescence analysis with an antibody to the extracellular domain of PDGFRb to detect surface PDGFRb-GFP. Cells were then re-fixed, permeabilized, and subjected to immunofluorescence analysis to detect GOLPH3. Analysis was restricted to cells over-expressing GOLPH3. (B) Quantification of (A) to measure cell surface PDGFRb-GFP relative to overall expression assessed by GFP levels. (C) Quantification of relative GOLPH3 expression. (D) Cells were transfected with the indicated siRNAs and either expression vector for GOLPH3 (WT) or empty vector. Cells were serum starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes), and analyzed by Western blotting using the indicated antibodies. (E) Quantification of (D) for phospho-AKT normalized to total AKT. Means and 95% confidence intervals are indicated for data pooled from (A) three independent experiments, with number of cells (n) as indicated and (D) individual biological replicates indicated by red dots. **p<0.01, ***p<0.001, ****p<0.0001 determined by two-sided t-test. n.s. indicates not statistically significant, p>0.05. *p<0.05 by one sample t-test (compared to the normalized control value, which was set at 1). p-values were calculated with Holm-Bonferroni correction for multiple comparisons.

Folders contain experimental replicates similar to Figure_4. Immunofluorescence tiffs are labelled via well and all other information is extraneous. Ex. W1 is Well 1, W2 is Well 2, etc. A separate excel spreadsheet in each folder is provided to describe the treatments performed in each well. An additional Excel spreadsheet provides quantification of the indicated measurements for each cell along with calculations and statistical analyses, as indicated by labeling within the file.

Figure 7A-B-C HeLa PDGFR-G OE G3 muts is for HeLa cells that overexpress GOLPH3 WT or mutants.

Figure 7D-E HeLa EGF M18AKD G3OE is for HeLa cells that overexpress MYO18A.

File: Figure_8.zip

Description: MYO18A is overexpressed in human cancers and correlates with RTK signaling.  (A to H) Box (median with 25^th and 75^th percentiles) and whisker (from min to max showing all points) plots are shown for relative MYO18A mRNA expression comparing normal and cancerous tissues from TCGA PanCancer Atlas studies, including pancreatic adenocarcinoma (n= 248 normal and n=181 tumor) (A), stomach adenocarcinoma (n = 294 normal and n = 375 tumor) (B), lung adenocarcinoma (n = 427 normal and n = 583 tumor) (C), acute myeloid leukemia (n = 407 normal and n = 151 tumor) (D), esophageal adenocarcinoma (n = 418 normal and n = 161 tumor) (E), rectal adenocarcinoma (n = 243 normal and n = 166 tumor) (F), hepatocellular carcinoma (n = 225 normal and n = 371 tumor) (G), renal papillary cell adenocarcinoma (n = 77 normal and n = 289 tumor) (H), breast invasive carcinoma (n = 582 normal and n = 1099 tumor) (I). Statistical significance was determined by Mann-Whitney test. **p<0.01, ****p<0.0001. (J) Volcano plot of reverse phase protein array data from 1084 invasive breast carcinoma samples from TCGA PanCancer Atlas indicating relative levels and statistical significance of the difference between proteins from cancer tissues with normal diploid compared to those with an amplified MYO18A gene.

Files can be opened with GraphPad Prism. For each gene, data for normal tissue and tumor are taken from previously published cancer cohorts are provided in the sheets for comparison.

File: Suppl_Figure_S1.zip

Description: EGFR signaling requires GOLPH3 and MYO18A in HeLa and other cells. (A) HeLa cells were transfected with the indicated siRNAs (including three independent sequences targeting GOLPH3), serum starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes), and subjected to Western blotting using the indicated antibodies. (B) Quantification of (A) for phosphorylated EGFR relative to total EGFR. (C) U87 glioma cells were transfected with the indicated siRNAs, serum starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes), and subjected to Western blotting using the indicated antibodies. Representative of 2 biological replicates per group. (D) HUVEC cells were transfected with the indicated siRNAs, serum starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes), and subjected to Western blotting using the indicated antibodies. Representative of 7 (control and GOLPH3 siRNAs) or 5 (MYO18A siRNA) biological replicates per group. (E) HeLa cells were transfected with the indicated siRNAs (including three independent sequences targeting MYO18A), serum starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes), and subjected to Western blotting using the indicated antibodies. (F) Quantification of (E) for phosphorylated EGFR relative to total EGFR. (G) HeLa cells were transfected with the indicated siRNAs, serum starved, stimulated (or not) with EGF (100 ng/ml for 2 minutes), and subjected to Western blotting using the indicated antibodies. (H) Quantification of (G) for total EGFR, AKT, or ERK relative to GAPDH for serum starved cells. Means and 95% confidence intervals are indicated for pooled data from multiple biological replicates, indicated as red dots. * p<0.05 by two-sided t-test with Holm-Bonferroni correction for multiple comparisons. n.s. indicates not statistically significant.

Folders are separated by letter and contain western blot images and Excel sheets with quantification/statistical analysis similar to Figure_1. File names of Western blot TIFF images indicate the specific antibody used to probe the membrane. Annotation within the images indicate which replicate experiment corresponds to each blot, and allows association with the data in the Excel spreadsheet. p - indicates phosphorylated protein. Tot - indicates Total protein.

File: Suppl_Figure_S2.zip

Description:  Validation of assay for detection of RTK trafficking and detection of EGFR recycling after knockdown. (A) Representative immunofluorescence confocal microscopy images of HeLa cells transfected with the indicated siRNAs and serum starved before fixation, permeabilization, and immunofluorescence to detect EGFR and DAPI staining to mark nuclei. siControl (n=125 cells), siGOLPH3 (n=116 cells), siMYO18A (n=91 cells). (B) Representative immunofluorescence confocal microscopy images of serum-starved HeLa cells transfected with GFP or PDGFRb-GFP. Cells were fixed and immunofluorescence analysis was performed with an antibody to the extracellular domain of PDGFRb and to GOLPH3. Differing conditions are shown in which cells are not permeabilized at all (NO PERM, n=128 cells), permeabilized from the start (PERM, n=200 cells), or not permeabilized until after using an antibody to the extracellular domain of PDGFRb and fixing that antibody in place (NP->P, n=289 cells for PDGFR-GFP and n = 190 cells for GFP). (C and D) Western blotting performed in parallel with the experiment shown in Figure 4A-B to validate knockdown of GOLPH3 or MYO18A. GAPDH was used as the loading control. n=3 biological replicates for each specific siRNA. (E) HeLa cells were transfected with indicated siRNA, starved for 16 hours before time 0, labelled with an antibody to the extracellular domain of the EGFR, and stimulated with 25 ng/mL of EGF to allow internalization. Excess antibody was removed by acid stripping and recycling of receptor back to the plasma membrane was evaluated at different time points after warming to 37ºC. (F) Quantification of surface EGFR on initial staining and after acid stripping in (E). (G) Quantification of recycled EGFR detected over time from (E). Means and 95% confidence intervals are graphed for data pooled from two independent experiments, with number of measured cells (n) indicated. Scale bars represent 10 mm. LUT indicates look up table changes. n.s. indicates not statistically significant.

Folders contain experimental replicates similar to WB images in Figure_1 and Immunofluorescence images in Figure_4.

Immunofluorescence tiffs are labelled via treatment group with the order as follows:

siRNA, time point, permeabilization protocol

Ex. MAX_KAS2kd g3 0strip nptp_2026-01-23_14.45.05.ims - KAS2kd g3 0strip nptp_2026-01-23_14.45.05.ims Resolution Level 1 is representative for cells that were treated with GOLPH3 siRNA, time 0 after stripping with acid, and underwent staining with the No Permeabilize the Permeabilize protocol.

med is Medium GC negative control siRNA, g3 is GOLPH3 siRNA

pre is for cells that were fixed before stripping

0strip is time 0 after stripping with acid, 5min is 5 minutes of recycling after stripping, 10 min is 10 minutes of recycling after stripping, etc.

No indicates no permeabilization performed.

NPTP indicates initial No Permeabilization (for exofacial staining), followed by Permeabilization.

Perm indicates permeabilization immediately after initial fixation.

File: Suppl_Figure_S3.zip

Description: Overexpression of GOLPH3 enhances growth factor signaling in MDA-MB-231 cells in a manner that depends on the ability of GOLPH3 to bind to PI4P. MDA-MB-231 cells were transfected with expression vectors for IRES-GFP, GOLPH3(WT)-IRES-GFP, or GOLPH3(R90L)-IRES-GFP, serum-starved, stimulated (or not) with EGF (100 ng/ml for 5 minutes), and analyzed by Western blotting using the indicated antibodies. The R90L mutation within the PI4P binding pocket strongly impairs the ability of GOLPH3 to bind to PI4P and localize to the Golgi. Representative of 8, 10, or 4 biological replicates per group, for control, GOLPH3, or GOLPH3(R90L), respectively.

Folders are separated by letter and contain western blot images and excel sheets with quantification/statistical analysis similar to Figure_1.

File: Suppl_Figure_S4.zip

Description: Overexpression of GOLPH3 does not affect EGFR recycling. (A) HeLa cells were transfected with expression vector for GOLPH3 (WT) or empty vector. 16 hours before time 0, cells were serum starved, labelled with an antibody to the extracellular domain of EGFR, and stimulated with 25 ng/mL of EGF to allow internalization. Excess antibody was removed by acid stripping and recycling of receptor back to the plasma membrane was evaluated at different time points after warming to 37ºC.  (B) Quantification of surface EGFR on initial staining and after acid stripping in (A). (C) Quantification of GOLPH3 expression in cells from (A). (D) Quantification of recycled EGFR detected over time from (A). Means and 95% confidence intervals are graphed for data pooled from two independent experiments, with number of measured cells (n) indicated. n.s. indicates not statistically significant. EV = Empty Vector. LUT indicates look up table changes.

Folder contain immunofluorescence images with naming convention similar to Suppl_Figure_S2

Ex. MAX_KAS3 g3 0strip perm_2026-01-22_16.57.42.ims - KAS3 g3 0strip perm_2026-01-22_16.57.42.ims Resolution Level 1 is representative for cells that overexpress GOLPH3 at the time point of 0min after stripping with acid and using the permeabilization protocol.

pc is empty vector pcDNA3.1 overexpression, g3 is GOLPH3 overexpression

pre is for cells that were fixed before stripping

0strip is time 0 after stripping with acid, 5min is 5 minutes of recycling after stripping, 10 min is 10 minutes of recycling after stripping, etc.

No indicates No permeabilization performed.

NPTP indicates initial No Permeabilization (for exofacial staining), followed by Permeabilization.

Perm indicates permeabilization immediately after initial fixation.

 

Code/software

All images can be opened in imageJ/FIJI. Some quantification files can be opened with GraphPad Prism 10 or newer.

Access information

Other publicly accessible locations of the data that the data was derived from:

• https://www.gtexportal.org/home/
• https://www.cancer.gov/ccg/research/genome-sequencing/tcga
• https://www.cancer.gov/ccg/research/genome-sequencing/target
• https://tnmplot.com/
• https://xenabrowser.net/datapages/
• https://www.cbioportal.org/