NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
Data files
Jan 19, 2022 version files 2.27 GB
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Fig1_and_SuppFig3.zip
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Fig2.zip
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Fig3.zip
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README.txt
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SuppFig1.zip
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SuppFig2.zip
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SuppFig4.zip
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SuppFig5.zip
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Abstract
We engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications. The ‘Genome-editing Optimized Locked Design’ (GOLD)-gRNA increases genome editing efficiency up to around 1000-fold (from 0.08% to 80.5%) with a mean increase across different other targets of 7.4-fold. The related NGS data is deposited here.