Data from: HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest
Data files
Apr 01, 2026 version files 155.69 KB
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Fig1_data.xlsx
12.80 KB
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Fig2_data.xlsx
16.51 KB
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Fig3_data.xlsx
15.56 KB
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Fig4_data.xlsx
9.88 KB
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Fig5_data.xlsx
36.03 KB
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Fig6_and_FIg6_sup_data.xlsx
62.22 KB
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README.md
2.69 KB
Abstract
This dataset supports the following findings: the HIV-1 Virion Infectivity Factor (Vif) promotes viral infection by targeting and degrading cellular APOBEC3 proteins, which are key regulators of intrinsic and innate antiviral immunity. Although Vif is also known to induce cell cycle arrest, its precise effects on the cell cycle have remained unclear. Using high-temporal-resolution single-cell live imaging and super-resolution microscopy, we found that Vif does not disrupt the G2/M boundary as previously proposed. Instead, Vif induces a distinct and robust pseudo-metaphase arrest, unlike the mild prometaphase arrest caused by Vpr. During this arrest, chromosomes initially align at the metaphase plate but later lose alignment, producing polar chromosomes. Notably, Vif markedly reduces Protein Phosphatase 1 (PP1) and Protein Phosphatase 2A (PP2A) levels at kinetochores, unlike Vpr. These findings reveal a novel role for Vif in kinetochore regulation and chromosome organization during mitosis.
https://doi.org/10.5061/dryad.k6djh9wj7
Description of the data and file structure
Raw datasets related to the manuscript (Ghone et al., 2025, HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest. eLife13:RP101136). These source datasets were used to generate the figures presented in Ghone et al (2025) "HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest", eLife (eLife-VOR-RA-2024-101136R1). The original article is fully and freely available to the public. Readers are encouraged to review the article together with these datasets, as the labels, variables, and definitions in the datasets correspond directly to the figures. Each dataset also provides figure numbers for convenient reference to the corresponding results.
- Fig1_data.xlsx: Fig 1B sheet includes mitotic duration (hours) in control, Vif, Vpr, and Vif+Vpr expressing cells. Fig 1C sheet includes frequency (%) of successful division, apoptotic cell death, and mitotic slippage in Vif, Vpr, and Vif+Vpr expressing cells.
- Fig2_data.xlsx: Fig 2C sheet includes metaphase index (%) in A-B columns, and mitotic duration (hours) in D-E columns. Fig 2E sheet includes mitotic duration (hours), Fig 2F sheet includes relative cell numbers Fig 2G sheet includes relative frequency of apoptotic cell death in control and conditional Vif-expressing cells.
- Fig3_data.xlsx: Fig 3B sheet includes duration (hours) of G1, S, and G2 in control and Vif expressing cells. Fig 3E includes mitotic duration (hours) in control and p53 KO cells with or without Vif expression. Fig 3G includes the time to metaphase (hours) after NEBD in control and p53 KO cells with or without Vif expression.
- Fig4_data.xlsx: Fig 4E sheet includes the spindle lengths (µm) in control and Vif-expressing cells.
- Fig5_data.xlsx: Fig 5C and 5F sheets include relative signal intensities of B56 (Fig 5C) and Plk1 (Fig 5F) in control and Vif expressing cells.
- Fig6_and_FIg6_sup_data.xlsx: Fig 6B sheets include relative signal intensities of Astrin and CENP-C. Fig 6C sheet includes the normalized signal intensities of Fig 6B sheet to sister kinetochore signals. Fig. 6E and 6F sheets include relative signal intensities of Hec1 pS55 (Fig 6E) and Hec1 (Fig 6F) signal intensities in control and Vif-expressing cells. Fig 6G sheets include the normalized signal intensities of Fig 6E-F sheets to sister kinetochore signals.