Previous work suggests that larvae from Sydney rock oysters that have been selectively bred for fast growth and disease resistance are more resilient to the impacts of ocean acidification than nonselected, wild-type oysters. In this study, we used proteomics to investigate the molecular differences between oyster populations in adult Sydney rock oysters and to identify whether these form the basis for observations seen in larvae. Adult oysters from a selective breeding line (B2) and nonselected wild types (WT) were exposed for 4 weeks to elevated pCO2 (856 μatm) before their proteomes were compared to those of oysters held under ambient conditions (375 μatm pCO2). Exposure to elevated pCO2 resulted in substantial changes in the proteomes of oysters from both the selectively bred and wild-type populations. When biological functions were assigned, these differential proteins fell into five broad, potentially interrelated categories of subcellular functions, in both oyster populations. These functional categories were energy production, cellular stress responses, the cytoskeleton, protein synthesis and cell signalling. In the wild-type population, proteins were predominantly upregulated. However, unexpectedly, these cellular systems were downregulated in the selectively bred oyster population, indicating cellular dysfunction. We argue that this reflects a trade-off, whereby an adaptive capacity for enhanced mitochondrial energy production in the selectively bred population may help to protect larvae from the effects of elevated CO2, whilst being deleterious to adult oysters.
Raw mass spec files from differentially expressed proteins of Sydney rock oysters - spot 211
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 211.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 213
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 213.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 214
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 214.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 306
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 306.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 308
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 308.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 502
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 502.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1007
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1007.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1404
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1404.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1405
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1405.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1406
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1406.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1407
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1407.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1607
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1607.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1705
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1705.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1706
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1706.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 1807
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 1807.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 2306
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 2306.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 2707
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 2707.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 2804
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 2804.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3110
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3110.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3304
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3304.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3305
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3305.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3405
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3405.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3407
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3407.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3603
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3603.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3707
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3707.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3708
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3708.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3806
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3806.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 3906
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 3906.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4103
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4103.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4303
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4303.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4404
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4404.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4405
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4405.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4508
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4508.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4510
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4510.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4609
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4609.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 4706
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 4706.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 5003
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 5003.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 5004
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 5004.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 5605
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 5605.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 5711
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 5711.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 5812
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 5812.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 5813
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 5813.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6104
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6104.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6105
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6105.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6405
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6405.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6406
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6406.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6505
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6505.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6608
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6608.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6805
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6805.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 6901
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 6901.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 7005
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 7005.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 7206
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 7206.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 7707
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 7707.raw
Raw mass spec files from differentially expressed proteins of Sydney rock oysters_spot 7803
Tryptic peptides were analysed by reversed phase nanoflow liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an QExactive mass spectrometer (Thermo scientific, CA, USA). Samples represent differentially expressed proteins from two populations of Sydney rock oyster at ambient and elevated CO2 exposure.
ET121113_spot 7803.raw
Normalised spot abundances
Normalised spot abundances for all protein spots across all treatments in the study.
Thompson et al normalised spot abundances.xlsx