Cortical VIP neurons as a critical node for dopamine actions
Data files
Nov 27, 2024 version files 3.47 GB
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1_VIP_Training.mat
127.20 MB
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1_VIP_Well_trained.mat
365.24 MB
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2_GRAB_Training.mat
3.16 MB
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2_GRAB_Well_trained_15s.mat
4.86 MB
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2_GRAB_Well_trained.mat
5.17 MB
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3_DTS_PYR_15s.mat
1.15 GB
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3_DTS_PYR_4s.mat
1.46 GB
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3_DTS_VIP_15s.mat
123.11 MB
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3_DTS_VIP_4s.mat
232.81 MB
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README.md
2.11 KB
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time_15s.mat
2.22 KB
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time.mat
1.50 KB
Abstract
Although dopamine modulates diverse cognitive processes of the prefrontal cortex including working memory, its underlying mechanisms are unclear. We investigated the roles of prefrontal vasoactive intestinal polypeptide (VIP)-expressing neurons, which are interneurons rich in D1 receptors (D1Rs), in working memory in mice engaged in a delayed match-to-sample task. VIP neurons conveyed robust working-memory signals and their inactivation profoundly impaired behavioral performance. Also, selective D1R knockdown in VIP neurons but not in pyramidal neurons impaired behavioral performance, indicating that VIP neurons are the primary mediators of dopamine effects on working memory. Additionally, we found that delay-period dopamine release dynamics vary depending on target location and that dopaminergic terminal stimulation increases prefrontal neuronal target selectivity in a laterality-dependent manner. These results indicate dopamine enhances working memory based on target laterality. These findings shed light on dopamine-modulated prefrontal neural processes underlying higher-order cognitive functions.
These datasets include raw calcium traces of VIP neurons and pyramidal neurons and raw GRAB-DA sensor traces of VIP-Cre mice in the mPFC during the delayed match-to-sample task.
Description of the data and file structure
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VIP recording data
1_VIP_Training, 208 cells
1_VIP_Well-trained, 684 cells
First column: Each cell calcium raw trace. Trials x time (refer time.mat file)
Second column: choice information (1, right correct; -1, left correct; 0.5, right error; -0.5, left error)
time, time 0 from delay onset (s), 4-s delay, delayed match-to-sample task.
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GRAB-DA data
2_GRAB_Training, 7 mice
2_GRAB_Well-trained, 10 mice
2_GRAB_Well-trained (15 s), 7 mice
First column: Each mouse GRAB raw trace. Trials x time (refer time.mat file)
Second column: choice information (1, right correct; -1, left correct; 0.5, right error; -0.5, left error)
time, time 0 from delay onset (s), 4-s delay, delayed match-to-sample task.
time_15s, time 0 from delay onset (s), 15-s delay, delayed match-to-sample task.
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DTS data
3_DTS_VIP_4s, 427 cells
3_DTS_PYR_4s, 3105 cells
3_DTS_VIP_15s, 174 cells
3_DTS_PYR_15s, 1579 cells
First column: Each cell calcium raw trace. Trials x time (refer time.mat file)
Second column: choice information (1, right correct; -1, left correct; 0.5, right error; -0.5, left error)
Third column: Light stimulation (1,n ON-trial; 0, OFF-trial)
4-s delay session, entire 4-s delay was stimulated; 15-s delay session, second-half delay 7.5 s was stimulated.time, time 0 from delay onset (s), 4-s delay, delayed match-to-sample task.
time_15s, time 0 from delay onset (s), 15-s delay, delayed match-to-sample task.
Sharing/Access information
Links to other publicly accessible locations of the data:
We implanted a GRIN lens with prism targeting layer 2/3 (VIP neuronal calcium imaging and dopamine-sensor imaging) or layer 5 (pyramidal neuronal calcium imaging) in the mPFC (2.0 mm ventral to brain surface). And recorded the Calcium signals and GRAB sensor signals in the mPFC during the delayed match-to-sample task.