Dynamic nuclear polarization (DNP) enables dramatic sensitivity enhancements of solid-state nuclear magnetic resonance (NMR) spectra for biological systems to be realized via polarization transfer from unpaired electrons of a polarizing agent, typically a nitroxide biradical, to nuclear spins of the system of interest. Here, we systematically investigate the prospect of recording DNP enhanced solid-state NMR spectra of hydrated lattice-like protein assemblies within sample formulations that are free of the commonly employed glycerol-based glassy solvent matrix containing exogenous polarizing agent molecules and instead consist of isotope-enriched protein of interest co-assembled with its dinitroxide-tagged structural analog incorporated as a dopant at low concentration. As a model system we use microcrystals of ¹³C,¹⁵N-labeled immunoglobulin-binding domain of protein G (GB1) doped with different amounts in the ∼1-16 % regime of a GB1 variant, 28R1/32R1, containing nitroxide spin labels at residues 28 and 32. For 28R1/32R1 dopant concentration of 4 %, equivalent to ca. 5 mM polarizing agent in the microcrystals, the DNP signal enhancement for ¹³C,¹⁵N-GB1 was found to be comparable to that obtained for model amino acid ¹³C,¹⁵N-proline in the usual glycerol/D₂O/H₂O matrix containing 5 mM 28R1/32R1 as the polarizing agent; further sensitivity increases were observed for 28R1/32R1 concentrations on the order of 10 %. Moreover, the spectral resolution for hydrated ¹³C,¹⁵N-GB1 microcrystals doped with 28R1/32R1 at ∼100 K was similar to that for a microcrystal suspension in glycerol/D₂O/H₂O with AMUPol biradical. This sample formulation approach based on doping with dinitroxide-tagged proteins is expected to be applicable to not only protein microcrystals but also to other lattice-like or filamentous assemblies composed of small to medium-size protein subunits, and further major improvements in sensitivity can be anticipated for this approach with continued development of optimal covalent nitroxide biradical DNP polarizing agent tags. The dataset contains 2D solid-state NMR spectra, EPR spectra and mass spectra.