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Dryad

A diversity arrays technology developed high-density DArT markers as a tool for efficient and rapid genomic assessment in Ethiopian Mustard (Brassica carinata A. Braun)

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Apr 17, 2025 version files 20.86 MB

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Abstract

High-density genomic markers were developed for Ethiopian mustard (Brassica carinata A. Braun), an allotetraploid species with a BBCC genome, using the Diversity Arrays Technology (DArT) genotype-by-sequencing (GBS) platform. From a redesigned 90 K Illumina Infinium SNP array incorporating additional 30,000 B genome information, 15,515 single nucleotide polymorphisms (SNPs) and 15,515 silicoDArT (dominant) markers were identified. Of these, 3,793 SNPs and 3,795 silicoDArT markers were polymorphic and were distributed across all 17 B. carinata chromosomes (8B, 9C). These markers offer a powerful tool for efficient genomic analysis, enabling rapid genomic screening, and targeted breeding strategies for B. carinata.