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Spermatozoa RRBS data: Post-Bismark aligned files extracted for the methylation call for every single C in the CpG context of sperm cells of Murrah buffalo bulls under heat stress

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Oct 01, 2024 version files 3.45 GB

Abstract

The DNA was isolated from purified spermatozoa samples obtained during hot summer season from the Murrah buffalo bulls (n=10) and were sequenced using RRBS. The animals belonged to two groups, seasonally affected (SA=5) and seasonally not affected (SNA=5), based on the semen quality parameters (sperm viability, hypo-osmotic swelling test, and acrosomal integrity), which significantly varied across the four seasons between the two groups. The study is the first of its kind to generate the methylome of heat stress affected Murrah buffalo bulls from the sperm cells. This data potentially paves the path to understanding the epigenetic regulation of heat stress on altered sperm function and semen quality.