DEGs affected by erucamide and predicted interaction between erucamide and indicated HrcC
Data files
Feb 10, 2025 version files 635.37 KB
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Dataset_S1-S9.zip
634.04 KB
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README.md
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Abstract
Numerous Gram-negative bacterial pathogens employ the type III secretion system (T3SS), a multi-protein injectisome, to deliver virulence proteins into host cells and cause diseases. We uncover erucamide as a previously unknown phytoalexin of both dicots and monocots that block the T3SS function of multiple bacterial pathogens. Genetically impairing erucamide accumulation or exogenous application in Arabidopsis highlighted erucamide’s role in anti-bacterial immunity. Erucamide binds HrcC, a key T3SS component, to block injectisome assembly. Analyses of erucamide analogues and HrcC mutants indicated that the erucamide-HrcC binding is required for inhibiting T3SS in vitro and anti-bacterial resistance in plants, suggesting an essential role of erucamide-HrcC binding in disease resistance. This work reveals a plant chemical defense that targets major virulence machinery in bacterial pathogens.
https://doi.org/10.5061/dryad.ns1rn8q3g
Description of the data and file structure
These datasets contain:
(1) Dataset S1: the differentially expressed genes following treatment with erucamide or between Col-0 and the mutants (FAAH-OE L1, faah-1, and kcs4/9/17-1 with high or low erucamide accumulation.
(2) Dataset S2-S9: Molecular docking and molecular dynamics simulation of erucamide interactions with indicated HrcC proteins or InvG.
Descriptions:
In Dataset S1
- Gene ID: TAIR accession.
- Average FPKM: Average counts per Fragments per million fragments mapped across three independent replication.
- Q value: Q value threshold of < 0.05 is used to identify significantly differentially expressed genes.
- Descriptions: the detailed information about the function of genes.
In Dataset S2-S9
The channels of the indicated proteins and molecules are referred to by their respective names. Datasets S2-S9 can be visualized using PyMol or online tool RCSB PDB (https://www.rcsb.org/3d-view).
Access information
Other publicly accessible locations of the data:
- n/a
Data was derived from the following sources:
*n/a